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- W2118062010 abstract "Three isomers of mono‐caffeoylquinic acid, specifically, 3‐ O ‐caffeoylquinic acid, 4‐ O ‐caffeoylquinic acid and 5‐ O ‐caffeoylquinic acid, were successfully isolated from a crude extract of tobacco ( Nicotiana tobaccum L.) wastes using continuous resin‐based pre‐separation and preparative high‐performance liquid chromatography ( HPLC ). The extract of tobacco wastes was continuously pre‐separated by resin‐based columns packed with D 101 and XAD ‐4, yielding total mono‐caffeoylquinic acids with a purity of 67.71% and a recovery rate of 90.06%. Variables affecting resolution and productivity of three mono‐caffeoylquinic acid isomers in preparative HPLC (i.e. mobile‐phase composition, pH, flow rate and loading amount) were studied. The optimum chromatographic conditions were determined to be a mobile phase consisting of 15% (v/v) methanol and aqueous acetic acid with a p H of 4.5, a flow rate of 4.0 mL/min, a loading amount of 4 mL and a detection wavelength of 360 nm. From 300 mg of loading sample, 56.3 mg of 3‐ O ‐caffeoylquinic acid, 92.8 mg of 5‐ O ‐caffeoylquinic acid and 73.1 mg of 4‐ O ‐caffeoylquinic acid were obtained in a single run, each with a purity of over 98% by HPLC . The structures of the isolated compounds were elucidated by ESI ‐ MS , 1 H-NMR and 13 C-NMR spectral data." @default.
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- W2118062010 date "2012-06-01" @default.
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- W2118062010 title "Isolation of mono-caffeoylquinic acids from tobacco waste using continuous resin-based pre-separation and preparative HPLC" @default.
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- W2118062010 doi "https://doi.org/10.1002/jssc.201101055" @default.
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