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• W2118097144 abstract "Intra- and interspecies variations of the translation elongation factor 1-α ( Tef-1α ) gene were evaluated as a new identification marker in a wide range of dermatophytes, which included 167 strains of 30 species. An optimized pan-dermatophyte primer pair was designed, and the target was sequenced. Consensus sequences were used for multiple alignment and phylogenetic tree analysis and the levels of intra- and interspecific nucleotide polymorphism were assessed. Between species, the analyzed part of the Tef-1α gene varied in length from 709 to 769 nucleotides. Significant numbers of species including Trichophyton rubrum, T. tonsurans , T. schoenleinii , T. concentricum , T. violaceum , Epidermophyton floccosum , Microsporum ferrugineum, M. canis , M. audouinii , T. equinum , T. eriotrephon , and T. erinacei were invariant in Tef-1α and had sufficient barcoding distance with neighboring species. Although overall consistency was found between ITS phylogeny as the current molecular marker of dermatophytes and Tef-1α , a higher discriminatory power of Tef-1α appeared particularly useful in some clades of closely related species such as the A. vanbreuseghemii , T. rubrum , A. benhamiae , and A. otae complexes. Nevertheless, we stress that a single gene can not specify species borderlines among dermatophytes and multiple lines of evidence based on a multilocus inquiry may ascertain an incontrovertible evaluation of kinship." @default.
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• W2118097144 date "2014-12-30" @default.
• W2118097144 modified "2023-10-18" @default.
• W2118097144 title "Translation elongation factor 1-α gene as a potential taxonomic and identification marker in dermatophytes" @default.
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• W2118097144 doi "https://doi.org/10.1093/mmy/myu088" @default.
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