FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2118375252> ?p ?o ?g. }

• W2118375252 endingPage "857" @default.
• W2118375252 startingPage "847" @default.
• W2118375252 abstract "We recently have demonstrated that EGF receptor (EGFR)-induced cell motility requires receptor kinase activity and autophosphorylation (P. Chen, K. Gupta, and A. Wells. 1994. J. Cell Biol. 124:547-555). This suggests that the immediate downstream effector molecule contains a src homology-2 domain. Phospholipase C gamma (PLC gamma) is among the candidate transducers of this signal because of its potential roles in modulating cytoskeletal dynamics. We utilized signaling-restricted EGFR mutants expressed in receptor devoid NR6 cells to determine if PLC activation is necessary for EGFR-mediated cell movement. Exposure to EGF (25 nM) augmented PLC activity in all five EGFR mutant cell lines which also responded by increased cell movement. Basal phosphoinositide turnover was not affected by EGF in the lines which do not present the enhanced motility response. The correlation between EGFR-mediated cell motility and PLC activity suggested, but did not prove, a causal link. A specific inhibitor of PLC, U73122 (1 microM) diminished both the EGF-induced motility and PLC responses, while its inactive analogue U73343 had no effect on these responses. Both the PLC and motility responses were decreased by expression of a dominant-negative PLC gamma-1 fragment in EGF-responsive infectant lines. Lastly, anti-sense oligonucleotides (20 microM) to PLC gamma-1 reduced both responses in NR6 cells expressing wild-type EGFR. These findings strongly support PLC gamma as the immediate post receptor effector in this motogenic pathway. We have demonstrated previously that EGFR-mediated cell motility and mitogenic signaling pathways are separable. The point of divergence is undefined. All kinase-active EGFR mutants induced the mitogenic response while only those which are autophosphorylated induced PLC activity. U73122 did not affect EGF-induced thymidine incorporation in these motility-responsive infectant cell lines. In addition, the dominant-negative PLC gamma-1 fragment did not diminish EGF-induced thymidine incorporation. All kinase active EGFR stimulated mitogen-activated protein (MAP) kinase activity, regardless of whether the receptors induced cell movement; this EGF-induced MAP kinase activity was not affected by U73122 at concentrations that depressed the motility response. Thus, the signaling pathways which lead to motility and cell proliferation diverge at the immediate post-receptor stage, and we suggest that this is accomplished by differential activation of effector molecules." @default.
• W2118375252 created "2016-06-24" @default.
• W2118375252 creator A5024476813 @default.
• W2118375252 creator A5028330659 @default.
• W2118375252 creator A5069693365 @default.
• W2118375252 creator A5076666408 @default.
• W2118375252 creator A5087720227 @default.
• W2118375252 date "1994-11-01" @default.
• W2118375252 modified "2023-10-01" @default.
• W2118375252 title "Epidermal growth factor receptor-mediated cell motility: phospholipase C activity is required, but mitogen-activated protein kinase activity is not sufficient for induced cell movement." @default.
• W2118375252 cites W1483470782 @default.
• W2118375252 cites W1487679352 @default.
• W2118375252 cites W1510677782 @default.
• W2118375252 cites W1536645610 @default.
• W2118375252 cites W1537076792 @default.
• W2118375252 cites W1539287293 @default.
• W2118375252 cites W1550035003 @default.
• W2118375252 cites W1552281474 @default.
• W2118375252 cites W1557403719 @default.
• W2118375252 cites W1560887909 @default.
• W2118375252 cites W1568858778 @default.
• W2118375252 cites W1573821584 @default.
• W2118375252 cites W1582821569 @default.
• W2118375252 cites W1599426550 @default.
• W2118375252 cites W1657721555 @default.
• W2118375252 cites W1720535299 @default.
• W2118375252 cites W1793635963 @default.
• W2118375252 cites W1854798937 @default.
• W2118375252 cites W1876543191 @default.
• W2118375252 cites W1966018982 @default.
• W2118375252 cites W1967882942 @default.
• W2118375252 cites W1972960760 @default.
• W2118375252 cites W1980166477 @default.
• W2118375252 cites W1997459814 @default.
• W2118375252 cites W2005395047 @default.
• W2118375252 cites W2007660746 @default.
• W2118375252 cites W2009556803 @default.
• W2118375252 cites W2021938232 @default.
• W2118375252 cites W2022129165 @default.
• W2118375252 cites W2024524102 @default.
• W2118375252 cites W2030463167 @default.
• W2118375252 cites W2031933070 @default.
• W2118375252 cites W2037434894 @default.
• W2118375252 cites W2053799953 @default.
• W2118375252 cites W2056681493 @default.
• W2118375252 cites W2061671663 @default.
• W2118375252 cites W2074096050 @default.
• W2118375252 cites W2074558153 @default.
• W2118375252 cites W2076606225 @default.
• W2118375252 cites W2083800765 @default.
• W2118375252 cites W2085495372 @default.
• W2118375252 cites W2091659591 @default.
• W2118375252 cites W2118678876 @default.
• W2118375252 cites W2126818181 @default.
• W2118375252 cites W2150069346 @default.
• W2118375252 cites W2150228169 @default.
• W2118375252 cites W2155958456 @default.
• W2118375252 cites W2157516468 @default.
• W2118375252 cites W2161155632 @default.
• W2118375252 cites W2164682544 @default.
• W2118375252 cites W2210799749 @default.
• W2118375252 cites W2252785765 @default.
• W2118375252 cites W2266038146 @default.
• W2118375252 doi "https://doi.org/10.1083/jcb.127.3.847" @default.
• W2118375252 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2120228" @default.
• W2118375252 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7962064" @default.
• W2118375252 hasPublicationYear "1994" @default.
• W2118375252 type Work @default.
• W2118375252 sameAs 2118375252 @default.
• W2118375252 citedByCount "319" @default.
• W2118375252 countsByYear W21183752522012 @default.
• W2118375252 countsByYear W21183752522013 @default.
• W2118375252 countsByYear W21183752522014 @default.
• W2118375252 countsByYear W21183752522015 @default.
• W2118375252 countsByYear W21183752522016 @default.
• W2118375252 countsByYear W21183752522017 @default.
• W2118375252 countsByYear W21183752522018 @default.
• W2118375252 countsByYear W21183752522019 @default.
• W2118375252 countsByYear W21183752522020 @default.
• W2118375252 countsByYear W21183752522021 @default.
• W2118375252 countsByYear W21183752522022 @default.
• W2118375252 countsByYear W21183752522023 @default.
• W2118375252 crossrefType "journal-article" @default.
• W2118375252 hasAuthorship W2118375252A5024476813 @default.
• W2118375252 hasAuthorship W2118375252A5028330659 @default.
• W2118375252 hasAuthorship W2118375252A5069693365 @default.
• W2118375252 hasAuthorship W2118375252A5076666408 @default.
• W2118375252 hasAuthorship W2118375252A5087720227 @default.
• W2118375252 hasBestOaLocation W21183752521 @default.
• W2118375252 hasConcept C150109051 @default.
• W2118375252 hasConcept C170493617 @default.
• W2118375252 hasConcept C184235292 @default.
• W2118375252 hasConcept C2776362946 @default.
• W2118375252 hasConcept C2778597717 @default.
• W2118375252 hasConcept C55493867 @default.
• W2118375252 hasConcept C58207958 @default.
• W2118375252 hasConcept C62478195 @default.
• W2118375252 hasConcept C86803240 @default.

• next