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• W2118575301 abstract "Plants naturally produce secondary metabolites that can be used as antimicrobials. The aim of this study was to assess the effects of <i>Psidium cattleianum</i> leaf extract on <i>Streptococcus mutans</i>. The extract (100%) was obtained by decoction of 100 g of leaves in 600 ml of deionized water. To assess killing, <i>S. mutans </i>biofilms were treated with water (negative control) or various extract dilutions [100, 50, 25% (v/v) in water] for 5 or 60 min. To evaluate the effect on protein expression, biofilms were exposed to water or 1.6% (v/v) extract for 120 min, proteins were extracted and submitted to 2-dimensional difference gel electrophoresis. Differentially expressed proteins were identified by mass spectrometry. The effect of 1.6% (v/v) extract on acid production was determined by pH measurements and compared to a water control. Viability was similar after 5 min of treatment with the 100% extract or 60 min with the 50% extract (about 0.03% survival). There were no differences in viability between the biofilms exposed to the 25 or 50% extract after 60 min of treatment (about 0.02% survival). Treatment with the 1.6% extract significantly changed protein expression. The abundance of 24 spots was decreased compared to water (p < 0.05). The extract significantly inhibited acid production (p < 0.05). It is concluded that <i>P. cattleianum</i> leaf extract kills <i>S. mutans</i> grown in biofilms when applied at high concentrations. At low concentrations it inhibits <i>S. mutans</i> acid production and reduces the expression of proteins involved in general metabolism, glycolysis and lactic acid production." @default.
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• W2118575301 date "2008-01-01" @default.
• W2118575301 modified "2023-09-30" @default.
• W2118575301 title "Effect of <i>Psidium cattleianum</i> Leaf Extract on <i>Streptococcus mutans</i> Viability, Protein Expression and Acid Production" @default.
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• W2118575301 doi "https://doi.org/10.1159/000121439" @default.
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