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- W2118589035 abstract "Plasmid propagation in populations of unstable, binary fission recombinant organisms has been studied using a segregated, population balance mathematical model. Segregated models have the advantage of direct incorporation of basic information on mechanisms and kinetics of plasmid replication and segregation at the single-cell level. The distribution of cellular plasmid content and specific rates of plasmid gene expression have been obtained for several single-cell models of plasmid replication, partition, and gene expression. Plasmid replication kinetics during cell growth significantly influence the plasmid content distribution. In the case of transient growth of plasmid-containing and plasmid-free cells in partially selective medium, the degree of selection required for stable maintenance of plasmid-containing cells has been determined. Guidelines are presented for applicability of simpler, nonsegregated models and for evaluation of the parameters in these models based on single-cell mechanisms and associated parameters." @default.
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- W2118589035 date "1985-02-01" @default.
- W2118589035 modified "2023-10-07" @default.
- W2118589035 title "A segregated model for plasmid content and product synthesis in unstable binary fission recombinant organisms" @default.
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- W2118589035 doi "https://doi.org/10.1002/bit.260270209" @default.
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