Matches in SemOpenAlex for { <https://semopenalex.org/work/W2118599710> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2118599710 endingPage "5944" @default.
- W2118599710 startingPage "5939" @default.
- W2118599710 abstract "Colchicine is a natural compound, which belongs to the botanical family Colchicaceae and prevents growth of cancer cells via antimitotic activity by interacting with microtubules. Although numerous studies have demonstrated that the effect of colchicine on cell apoptosis is mediated by the activation of caspase‑3, the signaling pathways involved in the process remain unknown. In the current study, evidence is presented regarding the missing information using HT‑29 human colon cancer cells. The effect of colchicine on apoptosis in HT‑29 cells and the apoptosis‑associated signaling pathways were determined using various methods, including cell viability assay, Annexin V/propidium idodide (PI) binding, PI staining, Hoechst 33342 staining, mitochondrial membrane potential (Δψm) assay, reactive oxygen species (ROS) assay and western blot analysis. Colchicine was observed to induce a dose‑dependent reduction in cell viability in HT‑29 cells and early apoptosis occurred when the cells were treated with 1 µg/ml colchicine. Furthermore, colchicine treatment induced a loss of Δψm, increased ROS production, activated caspase‑3, upregulated BAX expression and downregulated Bcl‑2 expression, which evidenced the colchicine activity on apoptosis, potentially by acting via the intrinsic apoptotic signaling pathway. Colchicine increased phosphorylation of p38, although not phosphorylation of extracellular signal-regulated kinase and c-Jun N-terminal kinase, which indicates that colchicine activates the p38 signaling pathway in order to induce cell apoptosis. Therefore, colchicine exhibited significant growth inhibition of the HT‑29 colon cancer cell line and induced apoptosis in the cells via the mitochondrial pathway, which is regulated by p38 signaling pathways." @default.
- W2118599710 created "2016-06-24" @default.
- W2118599710 creator A5033990407 @default.
- W2118599710 creator A5042553526 @default.
- W2118599710 creator A5054131406 @default.
- W2118599710 date "2015-08-12" @default.
- W2118599710 modified "2023-10-11" @default.
- W2118599710 title "Colchicine induces apoptosis in HT-29 human colon cancer cells via the AKT and c-Jun N-terminal kinase signaling pathways" @default.
- W2118599710 cites W1540916081 @default.
- W2118599710 cites W1971579914 @default.
- W2118599710 cites W1973424434 @default.
- W2118599710 cites W1975290731 @default.
- W2118599710 cites W1998721033 @default.
- W2118599710 cites W1999445090 @default.
- W2118599710 cites W2002550232 @default.
- W2118599710 cites W2009543520 @default.
- W2118599710 cites W2023205368 @default.
- W2118599710 cites W2024255080 @default.
- W2118599710 cites W2062406653 @default.
- W2118599710 cites W2064571598 @default.
- W2118599710 cites W2071959428 @default.
- W2118599710 cites W2103474120 @default.
- W2118599710 cites W2104293284 @default.
- W2118599710 cites W2107417780 @default.
- W2118599710 cites W2123868590 @default.
- W2118599710 cites W2128362969 @default.
- W2118599710 cites W2138137742 @default.
- W2118599710 cites W2144099695 @default.
- W2118599710 cites W2160082774 @default.
- W2118599710 doi "https://doi.org/10.3892/mmr.2015.4222" @default.
- W2118599710 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26299305" @default.
- W2118599710 hasPublicationYear "2015" @default.
- W2118599710 type Work @default.
- W2118599710 sameAs 2118599710 @default.
- W2118599710 citedByCount "36" @default.
- W2118599710 countsByYear W21185997102016 @default.
- W2118599710 countsByYear W21185997102017 @default.
- W2118599710 countsByYear W21185997102018 @default.
- W2118599710 countsByYear W21185997102019 @default.
- W2118599710 countsByYear W21185997102020 @default.
- W2118599710 countsByYear W21185997102021 @default.
- W2118599710 countsByYear W21185997102022 @default.
- W2118599710 countsByYear W21185997102023 @default.
- W2118599710 crossrefType "journal-article" @default.
- W2118599710 hasAuthorship W2118599710A5033990407 @default.
- W2118599710 hasAuthorship W2118599710A5042553526 @default.
- W2118599710 hasAuthorship W2118599710A5054131406 @default.
- W2118599710 hasBestOaLocation W21185997101 @default.
- W2118599710 hasConcept C153911025 @default.
- W2118599710 hasConcept C190283241 @default.
- W2118599710 hasConcept C502942594 @default.
- W2118599710 hasConcept C51551487 @default.
- W2118599710 hasConcept C53227056 @default.
- W2118599710 hasConcept C55493867 @default.
- W2118599710 hasConcept C57074206 @default.
- W2118599710 hasConcept C62478195 @default.
- W2118599710 hasConcept C75217442 @default.
- W2118599710 hasConcept C86803240 @default.
- W2118599710 hasConcept C95444343 @default.
- W2118599710 hasConceptScore W2118599710C153911025 @default.
- W2118599710 hasConceptScore W2118599710C190283241 @default.
- W2118599710 hasConceptScore W2118599710C502942594 @default.
- W2118599710 hasConceptScore W2118599710C51551487 @default.
- W2118599710 hasConceptScore W2118599710C53227056 @default.
- W2118599710 hasConceptScore W2118599710C55493867 @default.
- W2118599710 hasConceptScore W2118599710C57074206 @default.
- W2118599710 hasConceptScore W2118599710C62478195 @default.
- W2118599710 hasConceptScore W2118599710C75217442 @default.
- W2118599710 hasConceptScore W2118599710C86803240 @default.
- W2118599710 hasConceptScore W2118599710C95444343 @default.
- W2118599710 hasIssue "4" @default.
- W2118599710 hasLocation W21185997101 @default.
- W2118599710 hasLocation W21185997102 @default.
- W2118599710 hasOpenAccess W2118599710 @default.
- W2118599710 hasPrimaryLocation W21185997101 @default.
- W2118599710 hasRelatedWork W2025144955 @default.
- W2118599710 hasRelatedWork W2027244603 @default.
- W2118599710 hasRelatedWork W2060716574 @default.
- W2118599710 hasRelatedWork W2063262008 @default.
- W2118599710 hasRelatedWork W2088276717 @default.
- W2118599710 hasRelatedWork W2121295484 @default.
- W2118599710 hasRelatedWork W2365352466 @default.
- W2118599710 hasRelatedWork W2586593047 @default.
- W2118599710 hasRelatedWork W3024396134 @default.
- W2118599710 hasRelatedWork W3035328115 @default.
- W2118599710 hasVolume "12" @default.
- W2118599710 isParatext "false" @default.
- W2118599710 isRetracted "false" @default.
- W2118599710 magId "2118599710" @default.
- W2118599710 workType "article" @default.