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- W2118679804 abstract "Myzus persicae, more commonly known as green peach aphid, is an importantpest in agriculture. Each year it causes great losses to agricultural and horticultural plants.It feeds on sap in the phloem, the main transportation route for plant nutrients. It alsotransmits more than 100 plant viruses, and as there are still no agrichemicals specificallyagainst viruses, farmers often spray large amounts of pesticides to protect their crops.A group of widely-distributed plant proteins, termed lectins, have been recentlystudied because of their insecticidal properties against aphids. Snowdrop lectin, Galanthusnivalis agglutinin (GNA) was the first isolated monocot mannose-binding lectin,conferring properties of resistance to sap-sucking or homopteran pests.The aim of the project described in this thesis was to identify and express a novellectin in Arabidopsis thaliana and to assess the performance of the green peach aphid,Myzus persicae, on both transgenic and wild type plants. A gene encoding a novelmannose-binding lectin was cloned from Allium triquetrum (wild garlic). The full-lengthcDNA of Allium triquetrum agglutinin (ATA) was 719 bp and contained a 522 bp openreading frame encoding a 173 amino acid polypepetide. Homology analysis showed thatATA has high similarity with other mannose-binding lectins and includes three putativemannose-binding subdomains, which suggests that ATA may also confer resistance againstaphids. The pGreen 0029 vector and the 35S CaMV cassette were ligated to produce theexpression construct.An expression vector was engineered using the pGreen vector together withCaMV 35S promoter and the novel ATA gene was inserted. Transgenic Arabidopsisthaliana plants were subsequently generated using the Agrobacterium-mediated floral dipmethod. Six homozygous ATA-transformed lines and one empty vector-transformedcontrol line were obtained using kanamycin selection. Several key growth parameters ofthe transgenic plants were assessed to demonstrate that expressing ATA causes fewIIIphenotypic changes to the host plant. The transgenic plants were subsequently used foraphid bioassays, including choice and non-choice behaviour tests, Mean Relative GrowthRate test, fecundity test, and survival test. The aphid bioassays revealed that (1) ATA doesnot change the behaviour of aphids to choose a host; (2) Adults are more sensitive to ATAthan nymphs; (3) ATA has significant detrimental effects on aphid fecundity.The results described in the thesis show that the production of Allium triquetrumagglutinin in Arabidopsis confers partial resistance to the aphids, Myzus persicae. It islikely that this lectin in related Brassicaceae plants could be used, perhaps in conjunctionwith other pest-control measures in agriculture.Key words: Allium triquetrum agglutinin, aphid bioassay, Arabidopsis thaliana, choicetest, fecundity test, lectin, non-choice test, mean relative growth rate, Myzus persicae,pleiotropic effects, resistance against aphids, survival test." @default.
- W2118679804 created "2016-06-24" @default.
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- W2118679804 date "2009-01-01" @default.
- W2118679804 modified "2023-09-27" @default.
- W2118679804 title "Isolation and characterization of a novel lectin gene, Allium triquetrum agglutin, conferring insecticidal properties against Myzus persicae" @default.
- W2118679804 hasPublicationYear "2009" @default.
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