Matches in SemOpenAlex for { <https://semopenalex.org/work/W2118734291> ?p ?o ?g. }
- W2118734291 abstract "Fungi are important pathogens but challenging to enumerate using next-generation sequencing because of low absolute abundance in many samples and high levels of fungal DNA from contaminating sources.Here, we analyze fungal lineages present in the human airway using an improved method for contamination filtering. We use DNA quantification data, which are routinely acquired during DNA library preparation, to annotate output sequence data, and improve the identification and filtering of contaminants. We compare fungal communities and bacterial communities from healthy subjects, HIV+ subjects, and lung transplant recipients, providing a gradient of increasing lung impairment for comparison. We use deep sequencing to characterize ribosomal rRNA gene segments from fungi and bacteria in DNA extracted from bronchiolar lavage samples and oropharyngeal wash. Comparison to clinical culture data documents improved detection after applying the filtering procedure.We find increased representation of medically relevant organisms, including Candida, Cryptococcus, and Aspergillus, in subjects with increasingly severe pulmonary and immunologic deficits. We analyze covariation of fungal and bacterial taxa, and find that oropharyngeal communities rich in Candida are also rich in mitis group Streptococci,a community pattern associated with pathogenic polymicrobial biofilms. Thus, using this approach, it is possible to characterize fungal communities in the human respiratory tract more accurately and explore their interactions with bacterial communities in health and disease." @default.
- W2118734291 created "2016-06-24" @default.
- W2118734291 creator A5004580502 @default.
- W2118734291 creator A5016535188 @default.
- W2118734291 creator A5026370860 @default.
- W2118734291 creator A5032759284 @default.
- W2118734291 creator A5041936168 @default.
- W2118734291 creator A5042741758 @default.
- W2118734291 creator A5044285287 @default.
- W2118734291 creator A5053640758 @default.
- W2118734291 creator A5054786275 @default.
- W2118734291 creator A5056228937 @default.
- W2118734291 creator A5058503714 @default.
- W2118734291 creator A5066967353 @default.
- W2118734291 creator A5067519373 @default.
- W2118734291 creator A5074582058 @default.
- W2118734291 creator A5091021702 @default.
- W2118734291 date "2014-10-01" @default.
- W2118734291 modified "2023-10-18" @default.
- W2118734291 title "Improved characterization of medically relevant fungi in the human respiratory tract using next-generation sequencing" @default.
- W2118734291 cites W1491668332 @default.
- W2118734291 cites W1607979174 @default.
- W2118734291 cites W1964948648 @default.
- W2118734291 cites W1965509718 @default.
- W2118734291 cites W1966974215 @default.
- W2118734291 cites W1968206284 @default.
- W2118734291 cites W1980476578 @default.
- W2118734291 cites W2000542878 @default.
- W2118734291 cites W2004397336 @default.
- W2118734291 cites W2006509711 @default.
- W2118734291 cites W2010841805 @default.
- W2118734291 cites W2025060030 @default.
- W2118734291 cites W2031611770 @default.
- W2118734291 cites W2045073446 @default.
- W2118734291 cites W2050745388 @default.
- W2118734291 cites W2064812408 @default.
- W2118734291 cites W2072970694 @default.
- W2118734291 cites W2080720319 @default.
- W2118734291 cites W2085068503 @default.
- W2118734291 cites W2085987970 @default.
- W2118734291 cites W2088369864 @default.
- W2118734291 cites W2092681530 @default.
- W2118734291 cites W2104318549 @default.
- W2118734291 cites W2110345840 @default.
- W2118734291 cites W2114338344 @default.
- W2118734291 cites W2124351063 @default.
- W2118734291 cites W2127758025 @default.
- W2118734291 cites W2133211453 @default.
- W2118734291 cites W2134564677 @default.
- W2118734291 cites W2136527023 @default.
- W2118734291 cites W2138609440 @default.
- W2118734291 cites W2141980700 @default.
- W2118734291 cites W2143285077 @default.
- W2118734291 cites W2150180798 @default.
- W2118734291 cites W2153927784 @default.
- W2118734291 cites W2154026962 @default.
- W2118734291 cites W2155092137 @default.
- W2118734291 cites W2156800033 @default.
- W2118734291 cites W2159542223 @default.
- W2118734291 cites W2166559909 @default.
- W2118734291 cites W2167783345 @default.
- W2118734291 cites W2170196186 @default.
- W2118734291 cites W2770131614 @default.
- W2118734291 doi "https://doi.org/10.1186/s13059-014-0487-y" @default.
- W2118734291 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4232682" @default.
- W2118734291 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25344286" @default.
- W2118734291 hasPublicationYear "2014" @default.
- W2118734291 type Work @default.
- W2118734291 sameAs 2118734291 @default.
- W2118734291 citedByCount "107" @default.
- W2118734291 countsByYear W21187342912015 @default.
- W2118734291 countsByYear W21187342912016 @default.
- W2118734291 countsByYear W21187342912017 @default.
- W2118734291 countsByYear W21187342912018 @default.
- W2118734291 countsByYear W21187342912019 @default.
- W2118734291 countsByYear W21187342912020 @default.
- W2118734291 countsByYear W21187342912021 @default.
- W2118734291 countsByYear W21187342912022 @default.
- W2118734291 countsByYear W21187342912023 @default.
- W2118734291 crossrefType "journal-article" @default.
- W2118734291 hasAuthorship W2118734291A5004580502 @default.
- W2118734291 hasAuthorship W2118734291A5016535188 @default.
- W2118734291 hasAuthorship W2118734291A5026370860 @default.
- W2118734291 hasAuthorship W2118734291A5032759284 @default.
- W2118734291 hasAuthorship W2118734291A5041936168 @default.
- W2118734291 hasAuthorship W2118734291A5042741758 @default.
- W2118734291 hasAuthorship W2118734291A5044285287 @default.
- W2118734291 hasAuthorship W2118734291A5053640758 @default.
- W2118734291 hasAuthorship W2118734291A5054786275 @default.
- W2118734291 hasAuthorship W2118734291A5056228937 @default.
- W2118734291 hasAuthorship W2118734291A5058503714 @default.
- W2118734291 hasAuthorship W2118734291A5066967353 @default.
- W2118734291 hasAuthorship W2118734291A5067519373 @default.
- W2118734291 hasAuthorship W2118734291A5074582058 @default.
- W2118734291 hasAuthorship W2118734291A5091021702 @default.
- W2118734291 hasBestOaLocation W21187342911 @default.
- W2118734291 hasConcept C104317684 @default.
- W2118734291 hasConcept C105702510 @default.
- W2118734291 hasConcept C15151743 @default.
- W2118734291 hasConcept C207812311 @default.