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• W2118766963 abstract "A 95-kDa protein was isolated from Staphylococcus saprophyticus 7108 grown on dialysis membranes placed on the surface of brain heart infusion agar. Strain CCM883 did not produce this protein. Ultrathin sections revealed the presence of very thin, tuftlike, 50- to 75-nm-long structures on the surface of strain 7108, whereas strain CCM883 was comparably smooth. The surface material could be removed by digestion with proteinase K, suggesting that the surface structures contain protein. High-resolution scanning electron microscopy showed a thick layer of surface material on strain 7108, whereas strain CCM883 appeared smooth. The 95-kDa protein was purified by Sephacryl S-300 chromatography, and an antiserum was raised in rabbits. This antiserum was used in immunogold labeling experiments, which showed that the protein is associated with the surface structures. Our experiments thus demonstrate the presence of a fibrillar protein on the surface of S. saprophyticus (Ssp for S. saprophyticus surface-associated protein)." @default.
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• W2118766963 date "1992-03-01" @default.
• W2118766963 modified "2023-09-27" @default.
• W2118766963 title "Identification and characterization of a surface-associated protein (Ssp) of Staphylococcus saprophyticus" @default.
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• W2118766963 doi "https://doi.org/10.1128/iai.60.3.1055-1060.1992" @default.
• W2118766963 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/257593" @default.
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