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• W2118812014 abstract "A combination of plasmid curing and DNA-DNA hybridization data facilitated the identification of proteinase plasmids of 75 (pCI301) and 35 kilobases (pCI203) in the multi-plasmid-containing strains Lactococcus lactis subsp. lactis UC317 and L. lactis subsp. cremoris UC205, respectively. Both plasmids were transferred by conjugation to a plasmid-free background only after introduction of the conjugative streptococcal plasmid, pAMβ1. All Prt + transconjugants from matings involving either donor contained enlarged recombinant Prt plasmids. UC317-derived transconjugants were separable into different classes based on the presence of differently sized cointegrate plasmids and on segregation of the pCI301-derived Lac and Prt markers. All UC205-derived transconjugants harbored a single enlarged plasmid that was a cointegrate between pCI203 and pAMβ1. The identification of prt genes on pCI301 and pCI203 derivatives was achieved by a combination of restriction enzyme and hybridization analyses." @default.
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• W2118812014 date "1990-01-01" @default.
• W2118812014 modified "2023-09-23" @default.
• W2118812014 title "pAMβ1-Associated Mobilization of Proteinase Plasmids from <i>Lactococcus lactis</i> subsp. <i>lactis</i> UC317 and <i>L. lactis</i> subsp. <i>cremoris</i> UC205" @default.
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• W2118812014 doi "https://doi.org/10.1128/aem.56.1.195-201.1990" @default.
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