FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2118816331> ?p ?o ?g. }

• W2118816331 endingPage "897" @default.
• W2118816331 startingPage "893" @default.
• W2118816331 abstract "A DNA transfection protocol has been developed that makes use of the cyclic cationic amphipathic peptide gramicidin S and dioleoyl phosphatidylethanolamine. The DNA complex is formed by mixing gramicidin S with DNA at a 1:1 charge ratio and then adding phosphatidylethanolamine at a lipid/peptide molar ratio of 5:1. The complex mediates rapid association of DNA with cells and leads to transient expression levels of beta-galactosidase ranging from 1 to 30% of the transfected cells, with long-term expression being about an order of magnitude lower. The respective roles of peptide and phospholipid are not yet resolved but optimal transfection requires both the cyclic peptide and the hexagonal phase-competent phospholipid PtdEtn. Transfection in CV-1 cells is not affected by lysomotrophic agents, which suggests that DNA entry into the cell is via the plasma membrane. This technique that is simple, economical, and reproducible mediates transfection levels up to 20-fold higher than cationic liposomes in adherent mammalian cells." @default.
• W2118816331 created "2016-06-24" @default.
• W2118816331 creator A5004768213 @default.
• W2118816331 creator A5029647516 @default.
• W2118816331 date "1993-02-01" @default.
• W2118816331 modified "2023-10-14" @default.
• W2118816331 title "Cyclic amphipathic peptide-DNA complexes mediate high-efficiency transfection of adherent mammalian cells." @default.
• W2118816331 cites W123775848 @default.
• W2118816331 cites W1597819472 @default.
• W2118816331 cites W1893651774 @default.
• W2118816331 cites W1970812755 @default.
• W2118816331 cites W1971290197 @default.
• W2118816331 cites W1977152358 @default.
• W2118816331 cites W1980401440 @default.
• W2118816331 cites W1986815977 @default.
• W2118816331 cites W1997403350 @default.
• W2118816331 cites W2008842724 @default.
• W2118816331 cites W2014495927 @default.
• W2118816331 cites W2017391234 @default.
• W2118816331 cites W2067311836 @default.
• W2118816331 cites W2068583619 @default.
• W2118816331 cites W2071507204 @default.
• W2118816331 cites W2075656245 @default.
• W2118816331 cites W2077441973 @default.
• W2118816331 cites W2114918609 @default.
• W2118816331 cites W2118929543 @default.
• W2118816331 cites W2160327135 @default.
• W2118816331 cites W266995794 @default.
• W2118816331 doi "https://doi.org/10.1073/pnas.90.3.893" @default.
• W2118816331 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/45776" @default.
• W2118816331 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7679217" @default.
• W2118816331 hasPublicationYear "1993" @default.
• W2118816331 type Work @default.
• W2118816331 sameAs 2118816331 @default.
• W2118816331 citedByCount "89" @default.
• W2118816331 countsByYear W21188163312012 @default.
• W2118816331 countsByYear W21188163312014 @default.
• W2118816331 countsByYear W21188163312015 @default.
• W2118816331 countsByYear W21188163312017 @default.
• W2118816331 countsByYear W21188163312018 @default.
• W2118816331 countsByYear W21188163312019 @default.
• W2118816331 countsByYear W21188163312021 @default.
• W2118816331 crossrefType "journal-article" @default.
• W2118816331 hasAuthorship W2118816331A5004768213 @default.
• W2118816331 hasAuthorship W2118816331A5029647516 @default.
• W2118816331 hasBestOaLocation W21188163311 @default.
• W2118816331 hasConcept C104317684 @default.
• W2118816331 hasConcept C12554922 @default.
• W2118816331 hasConcept C153911025 @default.
• W2118816331 hasConcept C155887181 @default.
• W2118816331 hasConcept C174510640 @default.
• W2118816331 hasConcept C185154212 @default.
• W2118816331 hasConcept C185592680 @default.
• W2118816331 hasConcept C2776330855 @default.
• W2118816331 hasConcept C2778918659 @default.
• W2118816331 hasConcept C2779281246 @default.
• W2118816331 hasConcept C2779650302 @default.
• W2118816331 hasConcept C2781170229 @default.
• W2118816331 hasConcept C40767141 @default.
• W2118816331 hasConcept C41625074 @default.
• W2118816331 hasConcept C49923114 @default.
• W2118816331 hasConcept C54009773 @default.
• W2118816331 hasConcept C552990157 @default.
• W2118816331 hasConcept C55493867 @default.
• W2118816331 hasConcept C86803240 @default.
• W2118816331 hasConcept C92087593 @default.
• W2118816331 hasConcept C95444343 @default.
• W2118816331 hasConceptScore W2118816331C104317684 @default.
• W2118816331 hasConceptScore W2118816331C12554922 @default.
• W2118816331 hasConceptScore W2118816331C153911025 @default.
• W2118816331 hasConceptScore W2118816331C155887181 @default.
• W2118816331 hasConceptScore W2118816331C174510640 @default.
• W2118816331 hasConceptScore W2118816331C185154212 @default.
• W2118816331 hasConceptScore W2118816331C185592680 @default.
• W2118816331 hasConceptScore W2118816331C2776330855 @default.
• W2118816331 hasConceptScore W2118816331C2778918659 @default.
• W2118816331 hasConceptScore W2118816331C2779281246 @default.
• W2118816331 hasConceptScore W2118816331C2779650302 @default.
• W2118816331 hasConceptScore W2118816331C2781170229 @default.
• W2118816331 hasConceptScore W2118816331C40767141 @default.
• W2118816331 hasConceptScore W2118816331C41625074 @default.
• W2118816331 hasConceptScore W2118816331C49923114 @default.
• W2118816331 hasConceptScore W2118816331C54009773 @default.
• W2118816331 hasConceptScore W2118816331C552990157 @default.
• W2118816331 hasConceptScore W2118816331C55493867 @default.
• W2118816331 hasConceptScore W2118816331C86803240 @default.
• W2118816331 hasConceptScore W2118816331C92087593 @default.
• W2118816331 hasConceptScore W2118816331C95444343 @default.
• W2118816331 hasIssue "3" @default.
• W2118816331 hasLocation W21188163311 @default.
• W2118816331 hasLocation W21188163312 @default.
• W2118816331 hasLocation W21188163313 @default.
• W2118816331 hasLocation W21188163314 @default.
• W2118816331 hasOpenAccess W2118816331 @default.
• W2118816331 hasPrimaryLocation W21188163311 @default.
• W2118816331 hasRelatedWork W1977791762 @default.
• W2118816331 hasRelatedWork W2013373454 @default.
• W2118816331 hasRelatedWork W2063595779 @default.

• next