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- W2118827920 abstract "A 3.6-kilobase PstI fragment was isolated from a Vibrio cholerae chromosomal DNA library and shown to encode RecA-like activity in complementation studies with Escherichia coli recA mutants. Although DNA hybridization experiments failed to detect any homology between the E. coli and V. cholerae recA genes, hyperimmune antiserum produced against purified E. coli RecA protein recognized epitopes shared by the V. cholerae protein. The V. cholerae chromosomal fragments, when cloned and transferred to E. coli, provided the missing recA functions, including resistance to the alkylating agent methyl methanesulfonate, resistance to UV irradiation, and promotion of homologous recombination in Hfr mating experiments." @default.
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- W2118827920 date "1986-07-01" @default.
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- W2118827920 title "Isolation and characterization of the Vibrio cholerae recA gene" @default.
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- W2118827920 doi "https://doi.org/10.1128/jb.167.1.375-378.1986" @default.
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