Matches in SemOpenAlex for { <https://semopenalex.org/work/W2118839382> ?p ?o ?g. }
- W2118839382 endingPage "1665" @default.
- W2118839382 startingPage "1662" @default.
- W2118839382 abstract "The anomalous signal of cerium(III) ions present in a derivative of hen egg-white lysozyme (HEWL) crystals obtained by the addition of 0.025 M cerium chloride to the crystallization medium was used for phasing. X-ray intensity data were collected to 2 Å resolution using an in-house Cu K α radiation data-collection facility. Phasing of a single-wavelength data set purely based on its f ′′ led to a clearly interpretable electron-density map. Automated substructure solution by AutoSol in PHENIX resulted in four highest peaks corresponding to cerium(III) ions with data limited to 3 Å resolution, and about 90% of the residues were built automatically by AutoBuild in PHENIX . Cerium(III) ions bound on the surface of the enzyme are found to interact mainly with the main-chain and side-chain carbonyl groups of Asn, Glu, Tyr and Asp and with water molecules. Ce 3+ ions were used as potential anomalous scatterers for the in-house single-wavelength anomalous scattering technique, and this is proposed as a tool for macromolecular phasing and for the study of the interactions of trivalent metal ions with proteins and other macromolecules." @default.
- W2118839382 created "2016-06-24" @default.
- W2118839382 creator A5018304470 @default.
- W2118839382 creator A5040532393 @default.
- W2118839382 date "2011-11-25" @default.
- W2118839382 modified "2023-09-26" @default.
- W2118839382 title "In-house SAD phasing with surface-bound cerium ions" @default.
- W2118839382 cites W1987131278 @default.
- W2118839382 cites W2019219235 @default.
- W2118839382 cites W2021229113 @default.
- W2118839382 cites W2021582600 @default.
- W2118839382 cites W2025600392 @default.
- W2118839382 cites W2029069595 @default.
- W2118839382 cites W2029314552 @default.
- W2118839382 cites W2039616336 @default.
- W2118839382 cites W2057519548 @default.
- W2118839382 cites W2059222613 @default.
- W2118839382 cites W2079204867 @default.
- W2118839382 cites W2098515439 @default.
- W2118839382 cites W2101591373 @default.
- W2118839382 cites W2103714813 @default.
- W2118839382 cites W2105699258 @default.
- W2118839382 cites W2144081223 @default.
- W2118839382 cites W2145250443 @default.
- W2118839382 cites W2146563675 @default.
- W2118839382 cites W2151415053 @default.
- W2118839382 cites W2158113006 @default.
- W2118839382 cites W2159944520 @default.
- W2118839382 cites W2160324156 @default.
- W2118839382 cites W2169844674 @default.
- W2118839382 cites W2180229411 @default.
- W2118839382 cites W4255637261 @default.
- W2118839382 doi "https://doi.org/10.1107/s1744309111035718" @default.
- W2118839382 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3232165" @default.
- W2118839382 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22139192" @default.
- W2118839382 hasPublicationYear "2011" @default.
- W2118839382 type Work @default.
- W2118839382 sameAs 2118839382 @default.
- W2118839382 citedByCount "8" @default.
- W2118839382 countsByYear W21188393822012 @default.
- W2118839382 countsByYear W21188393822013 @default.
- W2118839382 countsByYear W21188393822015 @default.
- W2118839382 countsByYear W21188393822017 @default.
- W2118839382 crossrefType "journal-article" @default.
- W2118839382 hasAuthorship W2118839382A5018304470 @default.
- W2118839382 hasAuthorship W2118839382A5040532393 @default.
- W2118839382 hasBestOaLocation W21188393822 @default.
- W2118839382 hasConcept C120665830 @default.
- W2118839382 hasConcept C121332964 @default.
- W2118839382 hasConcept C127413603 @default.
- W2118839382 hasConcept C135393689 @default.
- W2118839382 hasConcept C138268822 @default.
- W2118839382 hasConcept C145148216 @default.
- W2118839382 hasConcept C154945302 @default.
- W2118839382 hasConcept C178790620 @default.
- W2118839382 hasConcept C179104552 @default.
- W2118839382 hasConcept C185592680 @default.
- W2118839382 hasConcept C191486275 @default.
- W2118839382 hasConcept C192562407 @default.
- W2118839382 hasConcept C199164860 @default.
- W2118839382 hasConcept C203036418 @default.
- W2118839382 hasConcept C204389451 @default.
- W2118839382 hasConcept C2777769593 @default.
- W2118839382 hasConcept C32909587 @default.
- W2118839382 hasConcept C41008148 @default.
- W2118839382 hasConcept C523597863 @default.
- W2118839382 hasConcept C55493867 @default.
- W2118839382 hasConcept C66938386 @default.
- W2118839382 hasConcept C8010536 @default.
- W2118839382 hasConcept C99679407 @default.
- W2118839382 hasConceptScore W2118839382C120665830 @default.
- W2118839382 hasConceptScore W2118839382C121332964 @default.
- W2118839382 hasConceptScore W2118839382C127413603 @default.
- W2118839382 hasConceptScore W2118839382C135393689 @default.
- W2118839382 hasConceptScore W2118839382C138268822 @default.
- W2118839382 hasConceptScore W2118839382C145148216 @default.
- W2118839382 hasConceptScore W2118839382C154945302 @default.
- W2118839382 hasConceptScore W2118839382C178790620 @default.
- W2118839382 hasConceptScore W2118839382C179104552 @default.
- W2118839382 hasConceptScore W2118839382C185592680 @default.
- W2118839382 hasConceptScore W2118839382C191486275 @default.
- W2118839382 hasConceptScore W2118839382C192562407 @default.
- W2118839382 hasConceptScore W2118839382C199164860 @default.
- W2118839382 hasConceptScore W2118839382C203036418 @default.
- W2118839382 hasConceptScore W2118839382C204389451 @default.
- W2118839382 hasConceptScore W2118839382C2777769593 @default.
- W2118839382 hasConceptScore W2118839382C32909587 @default.
- W2118839382 hasConceptScore W2118839382C41008148 @default.
- W2118839382 hasConceptScore W2118839382C523597863 @default.
- W2118839382 hasConceptScore W2118839382C55493867 @default.
- W2118839382 hasConceptScore W2118839382C66938386 @default.
- W2118839382 hasConceptScore W2118839382C8010536 @default.
- W2118839382 hasConceptScore W2118839382C99679407 @default.
- W2118839382 hasIssue "12" @default.
- W2118839382 hasLocation W21188393821 @default.
- W2118839382 hasLocation W21188393822 @default.
- W2118839382 hasLocation W21188393823 @default.
- W2118839382 hasLocation W21188393824 @default.