Matches in SemOpenAlex for { <https://semopenalex.org/work/W2118857462> ?p ?o ?g. }
- W2118857462 abstract "Recombinant retroviruses are effective vectors able to integrate transgenes into the target cell's genome to achieve longer-term expression. This study investigates the effect of cell lysis products, a common cell culture by-product, on the transduction of suspension cells by gammaretroviral vectors. Cell lysates derived from human and murine suspension cell lines significantly increased the transduction of human TF-1 and K-562 cell lines by gibbon ape leukemia virus-pseudotyped retroviral vectors without altering tropism. The transduction efficiency of TF-1 cells increased as a function of lysate concentration and decreased with increasing target cell concentrations. This was adequately predicted using a saturation equation based on the lysed-to-target cell concentration ratio, R, where: Fold increase = 1+Fold_(Max) (R/(K_(L)+R)). Lysate completely masked the effects of fibronectin when the two were added in combination. With protamine sulfate, the transduction efficiency was increased by lysate to 58% from 20% for protamine sulfate alone. Overall, the presence of cell lysate significantly influenced the outcome of the transduction process, either alone or in the presence of protamine sulfate or fibronectin." @default.
- W2118857462 created "2016-06-24" @default.
- W2118857462 creator A5025086319 @default.
- W2118857462 creator A5040936504 @default.
- W2118857462 creator A5050715885 @default.
- W2118857462 creator A5085736100 @default.
- W2118857462 date "2010-01-01" @default.
- W2118857462 modified "2023-09-27" @default.
- W2118857462 title "Effect of cell lysates on retroviral transduction efficiency of cells in suspension culture" @default.
- W2118857462 cites W1524529935 @default.
- W2118857462 cites W1750539994 @default.
- W2118857462 cites W1788853185 @default.
- W2118857462 cites W1908249423 @default.
- W2118857462 cites W1964178121 @default.
- W2118857462 cites W1968900557 @default.
- W2118857462 cites W1974333522 @default.
- W2118857462 cites W1974441013 @default.
- W2118857462 cites W1976246831 @default.
- W2118857462 cites W1982029014 @default.
- W2118857462 cites W1990555479 @default.
- W2118857462 cites W1997582694 @default.
- W2118857462 cites W2020110316 @default.
- W2118857462 cites W2023717115 @default.
- W2118857462 cites W2025032382 @default.
- W2118857462 cites W2028305959 @default.
- W2118857462 cites W2031228320 @default.
- W2118857462 cites W2035695825 @default.
- W2118857462 cites W2044288057 @default.
- W2118857462 cites W2045626020 @default.
- W2118857462 cites W2052575262 @default.
- W2118857462 cites W2058416269 @default.
- W2118857462 cites W2064216963 @default.
- W2118857462 cites W2067229421 @default.
- W2118857462 cites W2067381881 @default.
- W2118857462 cites W2076565991 @default.
- W2118857462 cites W2078975769 @default.
- W2118857462 cites W2082795668 @default.
- W2118857462 cites W2086664962 @default.
- W2118857462 cites W2088240772 @default.
- W2118857462 cites W2089894429 @default.
- W2118857462 cites W2102500159 @default.
- W2118857462 cites W2105007068 @default.
- W2118857462 cites W2118944608 @default.
- W2118857462 cites W2136381210 @default.
- W2118857462 cites W2138900159 @default.
- W2118857462 cites W2138977668 @default.
- W2118857462 cites W2145324624 @default.
- W2118857462 cites W2149547604 @default.
- W2118857462 cites W2150619432 @default.
- W2118857462 cites W2151024261 @default.
- W2118857462 cites W2155776362 @default.
- W2118857462 cites W2171120638 @default.
- W2118857462 cites W2328438661 @default.
- W2118857462 cites W2399105930 @default.
- W2118857462 cites W2409770357 @default.
- W2118857462 cites W3023211482 @default.
- W2118857462 cites W4229992754 @default.
- W2118857462 doi "https://doi.org/10.1002/bit.22634" @default.
- W2118857462 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20014140" @default.
- W2118857462 hasPublicationYear "2010" @default.
- W2118857462 type Work @default.
- W2118857462 sameAs 2118857462 @default.
- W2118857462 citedByCount "0" @default.
- W2118857462 crossrefType "journal-article" @default.
- W2118857462 hasAuthorship W2118857462A5025086319 @default.
- W2118857462 hasAuthorship W2118857462A5040936504 @default.
- W2118857462 hasAuthorship W2118857462A5050715885 @default.
- W2118857462 hasAuthorship W2118857462A5085736100 @default.
- W2118857462 hasConcept C1491633281 @default.
- W2118857462 hasConcept C15152581 @default.
- W2118857462 hasConcept C153911025 @default.
- W2118857462 hasConcept C159047783 @default.
- W2118857462 hasConcept C2522874641 @default.
- W2118857462 hasConcept C2777557582 @default.
- W2118857462 hasConcept C2779354726 @default.
- W2118857462 hasConcept C2780835560 @default.
- W2118857462 hasConcept C35693153 @default.
- W2118857462 hasConcept C54355233 @default.
- W2118857462 hasConcept C55493867 @default.
- W2118857462 hasConcept C57409179 @default.
- W2118857462 hasConcept C62112901 @default.
- W2118857462 hasConcept C81885089 @default.
- W2118857462 hasConcept C86803240 @default.
- W2118857462 hasConcept C95444343 @default.
- W2118857462 hasConceptScore W2118857462C1491633281 @default.
- W2118857462 hasConceptScore W2118857462C15152581 @default.
- W2118857462 hasConceptScore W2118857462C153911025 @default.
- W2118857462 hasConceptScore W2118857462C159047783 @default.
- W2118857462 hasConceptScore W2118857462C2522874641 @default.
- W2118857462 hasConceptScore W2118857462C2777557582 @default.
- W2118857462 hasConceptScore W2118857462C2779354726 @default.
- W2118857462 hasConceptScore W2118857462C2780835560 @default.
- W2118857462 hasConceptScore W2118857462C35693153 @default.
- W2118857462 hasConceptScore W2118857462C54355233 @default.
- W2118857462 hasConceptScore W2118857462C55493867 @default.
- W2118857462 hasConceptScore W2118857462C57409179 @default.
- W2118857462 hasConceptScore W2118857462C62112901 @default.
- W2118857462 hasConceptScore W2118857462C81885089 @default.
- W2118857462 hasConceptScore W2118857462C86803240 @default.
- W2118857462 hasConceptScore W2118857462C95444343 @default.