Matches in SemOpenAlex for { <https://semopenalex.org/work/W2118865529> ?p ?o ?g. }
- W2118865529 endingPage "625" @default.
- W2118865529 startingPage "611" @default.
- W2118865529 abstract "Budding yeast telomeres and cryptic mating-type loci are enriched at the nuclear envelope, forming foci that sequester silent information regulators (SIR factors), much as heterochromatic chromocenters in higher eukaryotes sequester HP1. Here we examine the impact of such subcompartments for regulating transcription genome-wide. We show that the efficiency of subtelomeric reporter gene repression depends not only on the strength of SIR factor recruitment by cis -acting elements, but also on the accumulation of SIRs in such perinuclear foci. To monitor the effects of disrupting this subnuclear compartment, we performed microarray analyses under conditions that eliminate telomere anchoring, while preserving SIR complex integrity. We found 60 genes reproducibly misregulated. Among those with increased expression, 22% were within 20 kb of a telomere, confirming that the nuclear envelope (NE) association of telomeres helps repress natural subtelomeric genes. In contrast, loci that were down-regulated were distributed over all chromosomes. Half of this ectopic repression was SIR complex dependent. We conclude that released SIR factors can promiscuously repress transcription at nontelomeric genes despite the presence of “anti-silencing” mechanisms. Bioinformatic analysis revealed that promoters bearing the PAC (RNA P olymerase A and C promoters) or Abf1 binding consenses are consistently down-regulated by mislocalization of SIR factors. Thus, the normal telomeric sequestration of SIRs both favors subtelomeric repression and prevents promiscuous effects at a distinct subset of promoters. This demonstrates that patterns of gene expression can be regulated by changing the spatial distribution of repetitive DNA sequences that bind repressive factors." @default.
- W2118865529 created "2016-06-24" @default.
- W2118865529 creator A5017960972 @default.
- W2118865529 creator A5040845783 @default.
- W2118865529 creator A5082924650 @default.
- W2118865529 creator A5083743794 @default.
- W2118865529 creator A5085000737 @default.
- W2118865529 creator A5089498360 @default.
- W2118865529 date "2009-01-29" @default.
- W2118865529 modified "2023-10-09" @default.
- W2118865529 title "The functional importance of telomere clustering: Global changes in gene expression result from SIR factor dispersion" @default.
- W2118865529 cites W1528082486 @default.
- W2118865529 cites W1530113860 @default.
- W2118865529 cites W1539988911 @default.
- W2118865529 cites W1595134057 @default.
- W2118865529 cites W1658331573 @default.
- W2118865529 cites W1895267024 @default.
- W2118865529 cites W1964866269 @default.
- W2118865529 cites W1965573381 @default.
- W2118865529 cites W1966072209 @default.
- W2118865529 cites W1971561688 @default.
- W2118865529 cites W1972789688 @default.
- W2118865529 cites W1973311604 @default.
- W2118865529 cites W1974057553 @default.
- W2118865529 cites W1975290221 @default.
- W2118865529 cites W1982504879 @default.
- W2118865529 cites W1985544638 @default.
- W2118865529 cites W1987362169 @default.
- W2118865529 cites W1989854142 @default.
- W2118865529 cites W1990186602 @default.
- W2118865529 cites W1993570130 @default.
- W2118865529 cites W1994900084 @default.
- W2118865529 cites W1996923044 @default.
- W2118865529 cites W1997530113 @default.
- W2118865529 cites W2000737623 @default.
- W2118865529 cites W2003084652 @default.
- W2118865529 cites W2004010498 @default.
- W2118865529 cites W2006873092 @default.
- W2118865529 cites W2006901859 @default.
- W2118865529 cites W2013078522 @default.
- W2118865529 cites W2013947447 @default.
- W2118865529 cites W2021432006 @default.
- W2118865529 cites W2030776068 @default.
- W2118865529 cites W2031098287 @default.
- W2118865529 cites W2034564804 @default.
- W2118865529 cites W2036347938 @default.
- W2118865529 cites W2037164528 @default.
- W2118865529 cites W2038486012 @default.
- W2118865529 cites W2038774969 @default.
- W2118865529 cites W2039940539 @default.
- W2118865529 cites W2043009382 @default.
- W2118865529 cites W2044347050 @default.
- W2118865529 cites W2049364400 @default.
- W2118865529 cites W2051351724 @default.
- W2118865529 cites W2054084132 @default.
- W2118865529 cites W2058346978 @default.
- W2118865529 cites W2058402081 @default.
- W2118865529 cites W2060178292 @default.
- W2118865529 cites W2069156698 @default.
- W2118865529 cites W2077719564 @default.
- W2118865529 cites W2085110456 @default.
- W2118865529 cites W2087430129 @default.
- W2118865529 cites W2093228543 @default.
- W2118865529 cites W2093531054 @default.
- W2118865529 cites W2094051937 @default.
- W2118865529 cites W2095018331 @default.
- W2118865529 cites W2097982583 @default.
- W2118865529 cites W2098549100 @default.
- W2118865529 cites W2098665858 @default.
- W2118865529 cites W2101814873 @default.
- W2118865529 cites W2105192189 @default.
- W2118865529 cites W2105487944 @default.
- W2118865529 cites W2105714668 @default.
- W2118865529 cites W2106795964 @default.
- W2118865529 cites W2109203574 @default.
- W2118865529 cites W2110037042 @default.
- W2118865529 cites W2111195542 @default.
- W2118865529 cites W2112124900 @default.
- W2118865529 cites W2113324803 @default.
- W2118865529 cites W2113478190 @default.
- W2118865529 cites W2113555317 @default.
- W2118865529 cites W2114803986 @default.
- W2118865529 cites W2115219137 @default.
- W2118865529 cites W2116202869 @default.
- W2118865529 cites W2116887390 @default.
- W2118865529 cites W2118083369 @default.
- W2118865529 cites W2118785964 @default.
- W2118865529 cites W2120752254 @default.
- W2118865529 cites W2126217681 @default.
- W2118865529 cites W2128314618 @default.
- W2118865529 cites W2130085763 @default.
- W2118865529 cites W2133885656 @default.
- W2118865529 cites W2135394026 @default.
- W2118865529 cites W2141925464 @default.
- W2118865529 cites W2145047610 @default.
- W2118865529 cites W2146645063 @default.
- W2118865529 cites W2149438959 @default.
- W2118865529 cites W2149703257 @default.