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• W2118938877 endingPage "7277" @default.
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• W2118938877 abstract "In brain neurons, P- and Q-type Ca²⁺ channels both appear to include a class A α1 subunit. In spite of this similarity, these channels differ pharmacologically and biophysically, particularly in inactivation kinetics. The molecular basis for this difference is unclear. In heterologous systems, alternative splicing and ancillary β subunits have been shown to alter biophysical properties of channels containing a class A α1 subunit. To test the hypothesis that similar mechanisms are at work in native systems, P- and Q-type currents were characterized in acutely isolated rat neostriatal, medium spiny neurons and cortical pyramidal neurons using whole-cell voltage-clamp techniques. Cells were subsequently aspirated and subjected to single-cell RT-PCR (scRT-PCR) analysis of calcium channel α₁ and β (β_1–4) subunit expression. In both cortical and neostriatal neurons, P- and Q-type currents were found in cells expressing class A α₁subunit mRNA. Although P-type currents in cortical and neostriatal neurons were similar, Q-type currents differed significantly in inactivation kinetics. Notably, Q-type currents in neostriatal neurons were similar to P-type currents in inactivation rate. The variation in Q-type channel biophysics was correlated with β subunit expression. Neostriatal neurons expressed significantly higher levels of β_2a mRNA and lower levels of β_1b mRNA than cortical neurons. These findings are consistent with the association of β_2a and β_1b subunits with slow and fast inactivation, respectively. Analysis of α_1Asplice variants in the linker between domains I and II failed to provide an alternative explanation for the differences in inactivation rates. These findings are consistent with the hypothesis that the biophysical properties of Q-type channels are governed by β subunit isoforms and are separable from toxin sensitivity." @default.
• W2118938877 created "2016-06-24" @default.
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• W2118938877 date "1999-09-01" @default.
• W2118938877 modified "2023-10-11" @default.
• W2118938877 title "Properties of Q-Type Calcium Channels in Neostriatal and Cortical Neurons are Correlated with β Subunit Expression" @default.
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• W2118938877 doi "https://doi.org/10.1523/jneurosci.19-17-07268.1999" @default.
• W2118938877 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6782517" @default.
• W2118938877 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10460233" @default.
• W2118938877 hasPublicationYear "1999" @default.
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