FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2118972497> ?p ?o ?g. }

• W2118972497 endingPage "6838" @default.
• W2118972497 startingPage "6827" @default.
• W2118972497 abstract "The transsulfuration enzyme cystathionine gamma-synthase (CGS) catalyses the pyridoxal 5'-phosphate (PLP)-dependent gamma-replacement of O-succinyl-L-homoserine and L-cysteine, yielding L-cystathionine. The crystal structure of the Escherichia coli enzyme has been solved by molecular replacement with the known structure of cystathionine beta-lyase (CBL), and refined at 1.5 A resolution to a crystallographic R-factor of 20.0%. The enzyme crystallizes as an alpha4 tetramer with the subunits related by non-crystallographic 222 symmetry. The spatial fold of the subunits, with three functionally distinct domains and their quaternary arrangement, is similar to that of CBL. Previously proposed reaction mechanisms for CGS can be checked against the structural model, allowing interpretation of the catalytic and substrate-binding functions of individual active site residues. Enzyme-substrate models pinpoint specific residues responsible for the substrate specificity, in agreement with structural comparisons with CBL. Both steric and electrostatic designs of the active site seem to achieve proper substrate selection and productive orientation. Amino acid sequence and structural alignments of CGS and CBL suggest that differences in the substrate-binding characteristics are responsible for the different reaction chemistries. Because CGS catalyses the only known PLP-dependent replacement reaction at Cgamma of certain amino acids, the results will help in our understanding of the chemical versatility of PLP." @default.
• W2118972497 created "2016-06-24" @default.
• W2118972497 creator A5002986510 @default.
• W2118972497 creator A5034279174 @default.
• W2118972497 creator A5056520004 @default.
• W2118972497 creator A5063495689 @default.
• W2118972497 creator A5090049599 @default.
• W2118972497 date "1998-12-01" @default.
• W2118972497 modified "2023-10-01" @default.
• W2118972497 title "Crystal structure of Escherichia coli cystathionine γ-synthase at 1.5 Å resolution" @default.
• W2118972497 cites W1484895105 @default.
• W2118972497 cites W1501346314 @default.
• W2118972497 cites W1546896179 @default.
• W2118972497 cites W1575236332 @default.
• W2118972497 cites W1591215983 @default.
• W2118972497 cites W1932515760 @default.
• W2118972497 cites W1967999247 @default.
• W2118972497 cites W1973592190 @default.
• W2118972497 cites W1987814777 @default.
• W2118972497 cites W1992635853 @default.
• W2118972497 cites W2003300391 @default.
• W2118972497 cites W2008708467 @default.
• W2118972497 cites W2013083986 @default.
• W2118972497 cites W2015797544 @default.
• W2118972497 cites W2021724636 @default.
• W2118972497 cites W2025905719 @default.
• W2118972497 cites W2028231353 @default.
• W2118972497 cites W2035943254 @default.
• W2118972497 cites W2041654633 @default.
• W2118972497 cites W2042505702 @default.
• W2118972497 cites W2042987185 @default.
• W2118972497 cites W2049521571 @default.
• W2118972497 cites W2050237110 @default.
• W2118972497 cites W2051212851 @default.
• W2118972497 cites W2051579324 @default.
• W2118972497 cites W2052760626 @default.
• W2118972497 cites W2068381123 @default.
• W2118972497 cites W2068399399 @default.
• W2118972497 cites W2078248419 @default.
• W2118972497 cites W2080476827 @default.
• W2118972497 cites W2083821641 @default.
• W2118972497 cites W2084575458 @default.
• W2118972497 cites W2093046597 @default.
• W2118972497 cites W2106315897 @default.
• W2118972497 cites W2113403117 @default.
• W2118972497 cites W2116275717 @default.
• W2118972497 cites W2128956788 @default.
• W2118972497 cites W2158630348 @default.
• W2118972497 cites W2404462991 @default.
• W2118972497 cites W2417579485 @default.
• W2118972497 cites W4231270328 @default.
• W2118972497 cites W4241771146 @default.
• W2118972497 cites W87506139 @default.
• W2118972497 cites W87823410 @default.
• W2118972497 doi "https://doi.org/10.1093/emboj/17.23.6827" @default.
• W2118972497 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1171030" @default.
• W2118972497 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9843488" @default.
• W2118972497 hasPublicationYear "1998" @default.
• W2118972497 type Work @default.
• W2118972497 sameAs 2118972497 @default.
• W2118972497 citedByCount "85" @default.
• W2118972497 countsByYear W21189724972012 @default.
• W2118972497 countsByYear W21189724972013 @default.
• W2118972497 countsByYear W21189724972014 @default.
• W2118972497 countsByYear W21189724972015 @default.
• W2118972497 countsByYear W21189724972016 @default.
• W2118972497 countsByYear W21189724972017 @default.
• W2118972497 countsByYear W21189724972018 @default.
• W2118972497 countsByYear W21189724972019 @default.
• W2118972497 countsByYear W21189724972020 @default.
• W2118972497 countsByYear W21189724972021 @default.
• W2118972497 countsByYear W21189724972022 @default.
• W2118972497 countsByYear W21189724972023 @default.
• W2118972497 crossrefType "journal-article" @default.
• W2118972497 hasAuthorship W2118972497A5002986510 @default.
• W2118972497 hasAuthorship W2118972497A5034279174 @default.
• W2118972497 hasAuthorship W2118972497A5056520004 @default.
• W2118972497 hasAuthorship W2118972497A5063495689 @default.
• W2118972497 hasAuthorship W2118972497A5090049599 @default.
• W2118972497 hasBestOaLocation W21189724972 @default.
• W2118972497 hasConcept C104292427 @default.
• W2118972497 hasConcept C104317684 @default.
• W2118972497 hasConcept C107824862 @default.
• W2118972497 hasConcept C16525657 @default.
• W2118972497 hasConcept C181199279 @default.
• W2118972497 hasConcept C185592680 @default.
• W2118972497 hasConcept C18903297 @default.
• W2118972497 hasConcept C197957613 @default.
• W2118972497 hasConcept C2776376580 @default.
• W2118972497 hasConcept C2776834122 @default.
• W2118972497 hasConcept C2777166331 @default.
• W2118972497 hasConcept C2777269803 @default.
• W2118972497 hasConcept C2777289219 @default.
• W2118972497 hasConcept C2778886173 @default.
• W2118972497 hasConcept C2779201268 @default.
• W2118972497 hasConcept C2780859938 @default.
• W2118972497 hasConcept C41183919 @default.
• W2118972497 hasConcept C47701112 @default.

• next