FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2118994852> ?p ?o ?g. }

• W2118994852 endingPage "R2" @default.
• W2118994852 startingPage "R2" @default.
• W2118994852 abstract "Sequence specific RNA binding proteins are important regulators of gene expression. Several related crosslinking-based, high-throughput sequencing methods, including PAR-CLIP, have recently been developed to determine direct binding sites of global protein-RNA interactions. However, no studies have quantitatively addressed the contribution of background binding to datasets produced by these methods.We measured non-specific RNA background in PAR-CLIP data, demonstrating that covalently crosslinked background binding is common, reproducible and apparently universal among laboratories. We show that quantitative determination of background is essential for identifying targets of most RNA-binding proteins and can substantially improve motif analysis. We also demonstrate that by applying background correction to an RNA binding protein of unknown binding specificity, Caprin1, we can identify a previously unrecognized RNA recognition element not otherwise apparent in a PAR-CLIP study.Empirical background measurements of global RNA-protein crosslinking are a necessary addendum to other experimental controls, such as performing replicates, because covalently crosslinked background signals are reproducible and otherwise unavoidable. Recognizing and quantifying the contribution of background extends the utility of PAR-CLIP and can improve mechanistic understanding of protein-RNA specificity, protein-RNA affinity and protein-RNA association dynamics." @default.
• W2118994852 created "2016-06-24" @default.
• W2118994852 creator A5051989396 @default.
• W2118994852 creator A5084806844 @default.
• W2118994852 date "2014-01-01" @default.
• W2118994852 modified "2023-09-26" @default.
• W2118994852 title "Advancing the functional utility of PAR-CLIP by quantifying background binding to mRNAs and lncRNAs" @default.
• W2118994852 cites W1965544640 @default.
• W2118994852 cites W1966985603 @default.
• W2118994852 cites W1968569455 @default.
• W2118994852 cites W1975849543 @default.
• W2118994852 cites W1986620732 @default.
• W2118994852 cites W1986911150 @default.
• W2118994852 cites W1987102836 @default.
• W2118994852 cites W1999388654 @default.
• W2118994852 cites W2001320847 @default.
• W2118994852 cites W2010081999 @default.
• W2118994852 cites W2010762066 @default.
• W2118994852 cites W2014222299 @default.
• W2118994852 cites W2022231987 @default.
• W2118994852 cites W2025061468 @default.
• W2118994852 cites W2034073307 @default.
• W2118994852 cites W2034201993 @default.
• W2118994852 cites W2039876257 @default.
• W2118994852 cites W2041440237 @default.
• W2118994852 cites W2047333953 @default.
• W2118994852 cites W2048085228 @default.
• W2118994852 cites W2049402769 @default.
• W2118994852 cites W2050108172 @default.
• W2118994852 cites W2050433648 @default.
• W2118994852 cites W2052326665 @default.
• W2118994852 cites W2053003053 @default.
• W2118994852 cites W2054630268 @default.
• W2118994852 cites W2059646461 @default.
• W2118994852 cites W2063614307 @default.
• W2118994852 cites W2069015522 @default.
• W2118994852 cites W2073424121 @default.
• W2118994852 cites W2073427046 @default.
• W2118994852 cites W2089652064 @default.
• W2118994852 cites W2089927164 @default.
• W2118994852 cites W2091095457 @default.
• W2118994852 cites W2098870145 @default.
• W2118994852 cites W2102619694 @default.
• W2118994852 cites W2103273849 @default.
• W2118994852 cites W2106488393 @default.
• W2118994852 cites W2109821031 @default.
• W2118994852 cites W2114532236 @default.
• W2118994852 cites W2117092553 @default.
• W2118994852 cites W2124985265 @default.
• W2118994852 cites W2129216514 @default.
• W2118994852 cites W2132803382 @default.
• W2118994852 cites W2136312358 @default.
• W2118994852 cites W2136949432 @default.
• W2118994852 cites W2139044529 @default.
• W2118994852 cites W2139440265 @default.
• W2118994852 cites W2149573463 @default.
• W2118994852 cites W2159249646 @default.
• W2118994852 cites W2167866392 @default.
• W2118994852 cites W2168507741 @default.
• W2118994852 cites W2168759699 @default.
• W2118994852 cites W2169636286 @default.
• W2118994852 cites W3106930452 @default.
• W2118994852 doi "https://doi.org/10.1186/gb-2014-15-1-r2" @default.
• W2118994852 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4053780" @default.
• W2118994852 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24393468" @default.
• W2118994852 hasPublicationYear "2014" @default.
• W2118994852 type Work @default.
• W2118994852 sameAs 2118994852 @default.
• W2118994852 citedByCount "110" @default.
• W2118994852 countsByYear W21189948522014 @default.
• W2118994852 countsByYear W21189948522015 @default.
• W2118994852 countsByYear W21189948522016 @default.
• W2118994852 countsByYear W21189948522017 @default.
• W2118994852 countsByYear W21189948522018 @default.
• W2118994852 countsByYear W21189948522019 @default.
• W2118994852 countsByYear W21189948522020 @default.
• W2118994852 countsByYear W21189948522021 @default.
• W2118994852 countsByYear W21189948522022 @default.
• W2118994852 countsByYear W21189948522023 @default.
• W2118994852 crossrefType "journal-article" @default.
• W2118994852 hasAuthorship W2118994852A5051989396 @default.
• W2118994852 hasAuthorship W2118994852A5084806844 @default.
• W2118994852 hasBestOaLocation W21189948521 @default.
• W2118994852 hasConcept C104317684 @default.
• W2118994852 hasConcept C105580179 @default.
• W2118994852 hasConcept C107824862 @default.
• W2118994852 hasConcept C150194340 @default.
• W2118994852 hasConcept C153911025 @default.
• W2118994852 hasConcept C41282012 @default.
• W2118994852 hasConcept C54355233 @default.
• W2118994852 hasConcept C67705224 @default.
• W2118994852 hasConcept C70721500 @default.
• W2118994852 hasConcept C86803240 @default.
• W2118994852 hasConcept C96739231 @default.
• W2118994852 hasConceptScore W2118994852C104317684 @default.
• W2118994852 hasConceptScore W2118994852C105580179 @default.
• W2118994852 hasConceptScore W2118994852C107824862 @default.
• W2118994852 hasConceptScore W2118994852C150194340 @default.

• next