FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2119015809> ?p ?o ?g. }

• W2119015809 endingPage "215" @default.
• W2119015809 startingPage "207" @default.
• W2119015809 abstract "The overwhelming majority of antibodies useful for formalin fixed, paraffin embedded (FFPE) tissues require antigen retrieval to reverse the effect of formalin fixation and re-establish immunoreactivity. How this reversal happens is poorly understood. We developed a new experimental model for studying the mechanism of formalin fixation and antigen retrieval. Epitope mapping studies on nine antibodies useful for FFPE tissues revealed that each consisted of a contiguous stretch of amino acids in the native protein (linear epitope). Small peptides representing the epitopes of antibodies to human epidermal growth factor receptor type (HER2), estrogen, and progesterone receptors were attached covalently to glass microscope slides in a peptide array. Most peptides retained immunoreactivity after formalin fixation. Immunoreactivity was completely abrogated for all peptides, however, if an irrelevant large protein was present during formalin-induced cross-linking. We hypothesize that cross-linking the irrelevant protein to the peptide epitopes sterically blocked antibodies from binding. Antigen retrieval dissociates irrelevant proteins and restores immunoreactivity. Because the epitopes for clinical antibodies require only primary protein structure, the fact that antigen retrieval probably denatures the secondary and tertiary structure of the protein is irrelevant. The same mechanism may occur in tissue samples subjected to formalin fixation and antigen retrieval." @default.
• W2119015809 created "2016-06-24" @default.
• W2119015809 creator A5002129390 @default.
• W2119015809 creator A5037863475 @default.
• W2119015809 creator A5044962058 @default.
• W2119015809 date "2009-01-01" @default.
• W2119015809 modified "2023-10-16" @default.
• W2119015809 title "Molecular mechanisms of antigen retrieval: antigen retrieval reverses steric interference caused by formalin-induced cross-links" @default.
• W2119015809 cites W101137784 @default.
• W2119015809 cites W12347272 @default.
• W2119015809 cites W1946994047 @default.
• W2119015809 cites W1971755928 @default.
• W2119015809 cites W1989220340 @default.
• W2119015809 cites W2005568246 @default.
• W2119015809 cites W2010547484 @default.
• W2119015809 cites W2060370518 @default.
• W2119015809 cites W2060937867 @default.
• W2119015809 cites W2080687440 @default.
• W2119015809 cites W2101030360 @default.
• W2119015809 cites W2102956462 @default.
• W2119015809 cites W2104872563 @default.
• W2119015809 cites W2107413762 @default.
• W2119015809 cites W2109753124 @default.
• W2119015809 cites W2131617728 @default.
• W2119015809 cites W2145799655 @default.
• W2119015809 cites W2163089952 @default.
• W2119015809 cites W2288763477 @default.
• W2119015809 cites W2406757460 @default.
• W2119015809 cites W2411022054 @default.
• W2119015809 cites W4230409310 @default.
• W2119015809 cites W4240756037 @default.
• W2119015809 cites W4244096700 @default.
• W2119015809 cites W4249604293 @default.
• W2119015809 doi "https://doi.org/10.3109/10520290903039078" @default.
• W2119015809 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4676902" @default.
• W2119015809 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19886757" @default.
• W2119015809 hasPublicationYear "2009" @default.
• W2119015809 type Work @default.
• W2119015809 sameAs 2119015809 @default.
• W2119015809 citedByCount "46" @default.
• W2119015809 countsByYear W21190158092013 @default.
• W2119015809 countsByYear W21190158092014 @default.
• W2119015809 countsByYear W21190158092015 @default.
• W2119015809 countsByYear W21190158092016 @default.
• W2119015809 countsByYear W21190158092017 @default.
• W2119015809 countsByYear W21190158092019 @default.
• W2119015809 countsByYear W21190158092020 @default.
• W2119015809 countsByYear W21190158092021 @default.
• W2119015809 countsByYear W21190158092022 @default.
• W2119015809 countsByYear W21190158092023 @default.
• W2119015809 crossrefType "journal-article" @default.
• W2119015809 hasAuthorship W2119015809A5002129390 @default.
• W2119015809 hasAuthorship W2119015809A5037863475 @default.
• W2119015809 hasAuthorship W2119015809A5044962058 @default.
• W2119015809 hasBestOaLocation W21190158092 @default.
• W2119015809 hasConcept C147483822 @default.
• W2119015809 hasConcept C153911025 @default.
• W2119015809 hasConcept C159654299 @default.
• W2119015809 hasConcept C185592680 @default.
• W2119015809 hasConcept C195616568 @default.
• W2119015809 hasConcept C203014093 @default.
• W2119015809 hasConcept C204232928 @default.
• W2119015809 hasConcept C2779281246 @default.
• W2119015809 hasConcept C2779484032 @default.
• W2119015809 hasConcept C55493867 @default.
• W2119015809 hasConcept C86803240 @default.
• W2119015809 hasConceptScore W2119015809C147483822 @default.
• W2119015809 hasConceptScore W2119015809C153911025 @default.
• W2119015809 hasConceptScore W2119015809C159654299 @default.
• W2119015809 hasConceptScore W2119015809C185592680 @default.
• W2119015809 hasConceptScore W2119015809C195616568 @default.
• W2119015809 hasConceptScore W2119015809C203014093 @default.
• W2119015809 hasConceptScore W2119015809C204232928 @default.
• W2119015809 hasConceptScore W2119015809C2779281246 @default.
• W2119015809 hasConceptScore W2119015809C2779484032 @default.
• W2119015809 hasConceptScore W2119015809C55493867 @default.
• W2119015809 hasConceptScore W2119015809C86803240 @default.
• W2119015809 hasIssue "5" @default.
• W2119015809 hasLocation W21190158091 @default.
• W2119015809 hasLocation W21190158092 @default.
• W2119015809 hasLocation W21190158093 @default.
• W2119015809 hasLocation W21190158094 @default.
• W2119015809 hasOpenAccess W2119015809 @default.
• W2119015809 hasPrimaryLocation W21190158091 @default.
• W2119015809 hasRelatedWork W136042991 @default.
• W2119015809 hasRelatedWork W1930847001 @default.
• W2119015809 hasRelatedWork W2001522283 @default.
• W2119015809 hasRelatedWork W2149585862 @default.
• W2119015809 hasRelatedWork W2351779799 @default.
• W2119015809 hasRelatedWork W2584227427 @default.
• W2119015809 hasRelatedWork W2730150905 @default.
• W2119015809 hasRelatedWork W3036945308 @default.
• W2119015809 hasRelatedWork W4313680703 @default.
• W2119015809 hasRelatedWork W2026877143 @default.
• W2119015809 hasVolume "84" @default.
• W2119015809 isParatext "false" @default.
• W2119015809 isRetracted "false" @default.
• W2119015809 magId "2119015809" @default.

• next