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- W2119080212 abstract "We present a method for obtaining long haplotypes, of over 3 kb in length, using a short-read sequencer, Barcode-directed Assembly for Extra-long Sequences (BAsE-Seq). BAsE-Seq relies on transposing a template-specific barcode onto random segments of the template molecule and assembling the barcoded short reads into complete haplotypes. We applied BAsE-Seq on mixed clones of hepatitis B virus and accurately identified haplotypes occurring at frequencies greater than or equal to 0.4%, with >99.9% specificity. Applying BAsE-Seq to a clinical sample, we obtained over 9,000 viral haplotypes, which provided an unprecedented view of hepatitis B virus population structure during chronic infection. BAsE-Seq is readily applicable for monitoring quasispecies evolution in viral diseases." @default.
- W2119080212 created "2016-06-24" @default.
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- W2119080212 date "2014-11-01" @default.
- W2119080212 modified "2023-10-14" @default.
- W2119080212 title "BAsE-Seq: a method for obtaining long viral haplotypes from short sequence reads" @default.
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- W2119080212 doi "https://doi.org/10.1186/s13059-014-0517-9" @default.
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