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- W2119128969 abstract "This work presents a sol-gel based biosensor for atrazine determination which has been obtained by introducing the enzyme polyphenol oxidase from apple tissue in a sol-gel matrix. Apple tissue acts as a molecular recognition element. Atrazine is an inactive compound electrochemically; redox coupling of dopamine was used for studying atrazine behavior. Atrazine was determined by monitoring the inhibition power of polyphenol oxidase activity. The measurements were performed in 0.1 M KH2PO4-NaOH buffer (pH 7.5). The effect of various experimental parameters such as pH, concentration of buffer, concentration of dopamine, incubation time and matrix composition has been investigated for optimum analytical performance. The biosensor consisted of 10.3% (w/w) of apple tissue. The bioelectrode exhibits a linear response for dopamine and atrazine concentrations in the range of 5.66 × 10−6−2.27 × 10−3M and 1 × 10−5 −1 × 10−4 M with a detection limit of 4.2 × 10−6 and 5.5 × 10−6 M, respectively. A correlation coefficient of 0.9945 and a relative standard deviation (R.S.D.) of 3.29% for dopamine, 0.9944 and 3.69% for a trazine were achieved." @default.
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- W2119128969 date "2008-06-01" @default.
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- W2119128969 title "Sol-Gel-Derived Biosensor Based on Plant Tissue: The Inhibitory Effect of Atrazine on Polyphenol Oxidase Activity for Determination of Atrazine" @default.
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- W2119128969 doi "https://doi.org/10.1002/jccs.200800077" @default.
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