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- W2119161370 endingPage "596" @default.
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- W2119161370 abstract "Recent cloning of a rat brain phosphatidylinositol 3,4,5-trisphosphate binding protein, centaurin α, identified a novel gene family based on homology to an amino-terminal zinc-binding domain. In Saccharomyces cerevisiae, the protein with the highest homology to centaurin α is Gcs1p, the product of theGCS1 gene. GCS1 was originally identified as a gene conditionally required for the reentry of cells into the cell cycle after stationary phase growth. Gcs1p was previously characterized as a guanosine triphosphatase-activating protein for the small guanosine triphosphatase Arf1, and gcs1 mutants displayed vesicle-trafficking defects. Here, we have shown that similar to centaurin α, recombinant Gcs1p bound phosphoinositide-based affinity resins with high affinity and specificity. A novelGCS1 disruption strain (gcs1Δ) exhibited morphological defects, as well as mislocalization of cortical actin patches. gcs1Δ was hypersensitive to the actin monomer-sequestering drug, latrunculin-B. Synthetic lethality was observed between null alleles of GCS1 andSLA2, the gene encoding a protein involved in stabilization of the actin cytoskeleton. In addition, synthetic growth defects were observed between null alleles of GCS1 andSAC6, the gene encoding the yeast fimbrin homologue. Recombinant Gcs1p bound to actin filaments, stimulated actin polymerization, and inhibited actin depolymerization in vitro. These data provide in vivo and in vitro evidence that Gcs1p interacts directly with the actin cytoskeleton in S. cerevisiae." @default.
- W2119161370 created "2016-06-24" @default.
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- W2119161370 date "1999-03-01" @default.
- W2119161370 modified "2023-09-27" @default.
- W2119161370 title "<i>GCS1</i>, an Arf Guanosine Triphosphatase-activating Protein in<i>Saccharomyces cerevisiae</i>, Is Required for Normal Actin Cytoskeletal Organization In Vivo and Stimulates Actin Polymerization In Vitro" @default.
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- W2119161370 doi "https://doi.org/10.1091/mbc.10.3.581" @default.
- W2119161370 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/25189" @default.
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- W2119161370 hasPublicationYear "1999" @default.
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