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• W2119535180 endingPage "5039" @default.
• W2119535180 startingPage "5030" @default.
• W2119535180 abstract "Epstein-Barr virus (EBV) utilizes a completely different mode of DNA replication during the lytic cycle than that employed during latency. The latency origin of replication, ori-P, which functions in the replication of the latent episomal form of the EBV genome, requires only a single virally encoded protein, EBNA-1, for its activity. During the lytic cycle, a separate origin, ori-Lyt, is utilized. Relatively little is known about the trans-acting proteins involved in ori-Lyt replication. We established a cotransfection-replication assay to identify EBV genes whose products are required for replication of ori-Lyt. In this assay, a BamHI-H plasmid containing ori-Lyt was replicated in Vero cells cotransfected with the BamHI-H target, the three EBV lytic-cycle transactivators Zta, Rta, and Mta, and the EBV genome provided in the form of a set of six overlapping cosmid clones. By removing individual cosmids from the cotransfection mixture, we found that only three of the six cosmids were necessary for ori-Lyt replication. Subcloning of the essential cosmids led to the identification of six EBV genes that encode replication proteins. These genes and their functions (either known or predicted on the basis of sequence comparison with herpes simplex virus) are BALF5, the DNA polymerase; BALF2, the single-stranded DNA-binding protein homolog; BMRF1, the DNA polymerase processivity factor; BSLF1 and BBLF4, the primase and helicase homologs; and BBLF2/3, a potential homolog of the third component of the helicase-primase complex. In addition, ori-Lyt replication in this cotransfection assay was also dependent on one or more genes provided by the EBV SalI-F fragment and on the three lytic-cycle transactivators Zta, Rta, and Mta." @default.
• W2119535180 created "2016-06-24" @default.
• W2119535180 creator A5001513626 @default.
• W2119535180 creator A5033758256 @default.
• W2119535180 creator A5048052831 @default.
• W2119535180 date "1992-08-01" @default.
• W2119535180 modified "2023-10-18" @default.
• W2119535180 title "trans-acting requirements for replication of Epstein-Barr virus ori-Lyt" @default.
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• W2119535180 cites W1545230875 @default.
• W2119535180 cites W1547854436 @default.
• W2119535180 cites W1558071029 @default.
• W2119535180 cites W1566554479 @default.
• W2119535180 cites W1573217573 @default.
• W2119535180 cites W1590365839 @default.
• W2119535180 cites W1594478849 @default.
• W2119535180 cites W1595354886 @default.
• W2119535180 cites W1600356659 @default.
• W2119535180 cites W1608297032 @default.
• W2119535180 cites W1634217411 @default.
• W2119535180 cites W1652668664 @default.
• W2119535180 cites W1731704781 @default.
• W2119535180 cites W1755069884 @default.
• W2119535180 cites W1755591780 @default.
• W2119535180 cites W1777619326 @default.
• W2119535180 cites W1786573368 @default.
• W2119535180 cites W1827511896 @default.
• W2119535180 cites W1850517634 @default.
• W2119535180 cites W1883731485 @default.
• W2119535180 cites W1893025248 @default.
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• W2119535180 cites W1909649638 @default.
• W2119535180 cites W1910426815 @default.
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• W2119535180 cites W1931710801 @default.
• W2119535180 cites W1939536163 @default.
• W2119535180 cites W1948890517 @default.
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• W2119535180 cites W1976648018 @default.
• W2119535180 cites W1980464074 @default.
• W2119535180 cites W1980966767 @default.
• W2119535180 cites W1981922425 @default.
• W2119535180 cites W1985971484 @default.
• W2119535180 cites W2008163029 @default.
• W2119535180 cites W2009230618 @default.
• W2119535180 cites W2014669590 @default.
• W2119535180 cites W2015221134 @default.
• W2119535180 cites W2017883410 @default.
• W2119535180 cites W2021172417 @default.
• W2119535180 cites W2023445947 @default.
• W2119535180 cites W2029524323 @default.
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• W2119535180 cites W2042648413 @default.
• W2119535180 cites W2047197428 @default.
• W2119535180 cites W2056113253 @default.
• W2119535180 cites W2063450941 @default.
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• W2119535180 doi "https://doi.org/10.1128/jvi.66.8.5030-5039.1992" @default.
• W2119535180 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/241360" @default.
• W2119535180 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1321285" @default.
• W2119535180 hasPublicationYear "1992" @default.
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