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- W2119683187 abstract "Osteoblast proliferation is sensitive to the topography of material surfaces. In this study, the proliferation of MC3T3 E1-S14 osteoblast cells on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) films with different surface characteristics was investigated. The solvent cast films were prepared using three different solvents/solvent mixtures; chloroform, DCM and a mixture of chloroform and acetone which produced PHBV films with both a rough (at the air interface) and smooth (at the glass interface) surface. Investigation of the surface characteristics by scanning electron and scanning probe microscopies revealed different surface topographies and degrees of surface roughness ranging from 20 to 200 nm. Mapping of the surface crystallinity index by micro-attenuated total reflectance Fourier transform infrared (ATR-FTIR) showed distinct variations in surface crystallinity between the different film surfaces. Water contact angles were significantly higher on the rough surface compared the smooth surface for a particular substrate, however, all surfaces were hydrophobic in nature (θA was in the range 69 - 80 degrees). MC3T3 E1-S14 osteoblast cells were cultured on the six different surfaces and proliferation was determined. After 2 days cell proliferation on all surfaces was significantly less than on the control substrate, however, after 4 days cell proliferation was optimal on the three surfaces that displayed the highest contact angle and the smallest crystallinity heterogeneity. In addition, the surface roughness and more specifically the surface topography influenced the proliferation of osteoblast cells on the PHBV film surface." @default.
- W2119683187 created "2016-06-24" @default.
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- W2119683187 date "2007-09-01" @default.
- W2119683187 modified "2023-09-23" @default.
- W2119683187 title "Surface Roughness and Topography of Poly(3-hydroxybutyrate-co-3- hydroxyvalerate) Influences Osteoblast Cell Growth" @default.
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