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• W2119735670 abstract "MicroRNAs (miRNAs) are coordinators of cellular differentiation, including granulopoiesis. Although differential expression of many miRNAs is associated with the maturation of granulocytes, analysis of differentially expressed miRNAs and their cellular localization across all stages of granulopoiesis, starting from hemopoietic stems cells, is not well characterized.We analyzed whole cell miRNA and mRNA expression during granulopoiesis using Taqman low-density and Affymetrix arrays respectively. We also performed nuclear and cytoplasmic fractionation followed by Taqman low-density array and/or quantitative PCR to identify nuclear-enriched miRNAs in hemopoietic stem/progenitor cells, promyelocytes, myelocytes, granulocytes and several hemopoietic cell lines. Anti-correlation between the expression of miRNA and target pairs was used to determine putative miRNA targets.Analyses of our array data revealed distinct clusters of differentially expressed miRNAs that are specific to promyelocytes and granulocytes. While the roles of many of these miRNAs in granulopoiesis are not currently known, anti-correlation of the expression of miRNA/mRNA target pairs identified a suite of novel target genes. Clusters of miRNAs (including members of the let-7 and miR-17-92 families) are downregulated in hemopoietic stem/progenitor cells, potentially allowing the expression of target genes known to facilitate stem cell proliferation and homeostasis. Additionally, four miRNAs (miR-709, miR-706, miR-690 and miR-467a*) were found to be enriched in the nucleus of myeloid cells and multiple hemopoietic cell lines compared to other miRNAs, which are predominantly cytoplasmic-enriched. Both miR-709 and miR-706 are nuclear-enriched throughout granulopoiesis and have putative binding sites of extensive complementarity downstream of pri-miRNAs. Nuclear enrichment of miR-467a* is specific to hemopoietic stem/progenitors and promyelocytes. These miRNAs are also nuclear-enriched in other hemopoietic cell lines, where nuclear sequestering may fine-tune the expression of cytoplasmic mRNA targets.Overall, we have demonstrated differentially expressed miRNAs that have not previously been associated with hemopoietic differentiation and provided further evidence of regulated nuclear-enrichment of miRNAs. Further studies into miRNA function in granulocyte development may shed light on fundamental aspects of regulatory RNA biology and the role of nuclear miRNAs." @default.
• W2119735670 created "2016-06-24" @default.
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• W2119735670 date "2014-05-15" @default.
• W2119735670 modified "2023-10-17" @default.
• W2119735670 title "Identification of nuclear-enriched miRNAs during mouse granulopoiesis" @default.
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• W2119735670 cites W1964118535 @default.
• W2119735670 cites W1964403665 @default.
• W2119735670 cites W1967818875 @default.
• W2119735670 cites W1969003435 @default.
• W2119735670 cites W1969294374 @default.
• W2119735670 cites W1970829776 @default.
• W2119735670 cites W1972868741 @default.
• W2119735670 cites W1978077623 @default.
• W2119735670 cites W1980860344 @default.
• W2119735670 cites W1982205229 @default.
• W2119735670 cites W1982562602 @default.
• W2119735670 cites W1985098845 @default.
• W2119735670 cites W1985563073 @default.
• W2119735670 cites W1986903527 @default.
• W2119735670 cites W1989234423 @default.
• W2119735670 cites W1990848127 @default.
• W2119735670 cites W1992941735 @default.
• W2119735670 cites W1999341037 @default.
• W2119735670 cites W2002767435 @default.
• W2119735670 cites W2004280138 @default.
• W2119735670 cites W2005102860 @default.
• W2119735670 cites W2007633189 @default.
• W2119735670 cites W2012041376 @default.
• W2119735670 cites W2014974499 @default.
• W2119735670 cites W2017971576 @default.
• W2119735670 cites W2022724408 @default.
• W2119735670 cites W2028994878 @default.
• W2119735670 cites W2029071521 @default.
• W2119735670 cites W2029126296 @default.
• W2119735670 cites W2034325228 @default.
• W2119735670 cites W2036534625 @default.
• W2119735670 cites W2045496844 @default.
• W2119735670 cites W2048999577 @default.
• W2119735670 cites W2050855482 @default.
• W2119735670 cites W2057213628 @default.
• W2119735670 cites W2057549495 @default.
• W2119735670 cites W2058997632 @default.
• W2119735670 cites W2061638986 @default.
• W2119735670 cites W2062290101 @default.
• W2119735670 cites W2062465863 @default.
• W2119735670 cites W2063080163 @default.
• W2119735670 cites W2064698668 @default.
• W2119735670 cites W2069009920 @default.
• W2119735670 cites W2069691262 @default.
• W2119735670 cites W2075775205 @default.
• W2119735670 cites W2079625864 @default.
• W2119735670 cites W2080958451 @default.
• W2119735670 cites W2084400594 @default.
• W2119735670 cites W2091402406 @default.
• W2119735670 cites W2092018614 @default.
• W2119735670 cites W2094253263 @default.
• W2119735670 cites W2094915354 @default.
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• W2119735670 cites W2106637741 @default.
• W2119735670 cites W2106685590 @default.
• W2119735670 cites W2107507136 @default.
• W2119735670 cites W2116402236 @default.
• W2119735670 cites W2121412124 @default.
• W2119735670 cites W2125987139 @default.
• W2119735670 cites W2127632559 @default.
• W2119735670 cites W2130000937 @default.
• W2119735670 cites W2138425796 @default.
• W2119735670 cites W2149784145 @default.
• W2119735670 cites W2150892663 @default.
• W2119735670 cites W2151922790 @default.
• W2119735670 cites W2152333235 @default.
• W2119735670 cites W2162674813 @default.
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• W2119735670 cites W2165936126 @default.
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• W2119735670 doi "https://doi.org/10.1186/1756-8722-7-42" @default.
• W2119735670 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4046156" @default.
• W2119735670 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24886830" @default.
• W2119735670 hasPublicationYear "2014" @default.
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