FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2119785014> ?p ?o ?g. }

• W2119785014 abstract "The A673T mutation in the amyloid precursor protein (APP) protects against Alzheimer's disease by reducing β-amyloid protein (Aβ) production. This mutation reduced the release of the soluble APP fragment (sAPPβ), which is processed by β-secretase, suggesting a concomitant decrease in the β-carboxyl fragment of APP (C99), which is a direct substrate of γ-secretase for Aβ production. However, it remains controversial whether the level of C99 is significantly reduced in cells expressing APP that carry A673T as the cause of reduced Aβ production. Here, we investigated the effect of the A673T mutation in C99 on γ-cleavage in cells.We found that the level of C99 in cells expressing APP A673T was indistinctive of that observed in cells expressing wild-type APP, although the release of sAPPβ was significantly reduced in the APP A673T cells. In addition, our reconstituted β-secretase assay demonstrated no significant difference in β-cleavage on an APP fragment carrying the A673T mutation compared with the wild-type fragment. Importantly, cells expressing C99 containing the A673T mutation (C99 A2T; in accordance with the Aβ numbering) produced roughly half the level of Aβ compared with the wild-type C99, suggesting that the C99 A2T is an insufficient substrate of γ-secretase in cells. A cell-free γ-secretase assay revealed that Aβ production from the microsomal fraction of cells expressing C99 A2T was diminished. A sucrose gradient centrifugation analysis indicated that the levels of the C99 A2T that was codistributed with γ-secretase components in the raft fractions were reduced significantly.Our data indicate that the A673T mutation in APP alters the release of sAPPβ, but not the C99 level, and that the C99 A2T is an inefficient substrate for γ-secretase in cell-based assay." @default.
• W2119785014 created "2016-06-24" @default.
• W2119785014 creator A5016024085 @default.
• W2119785014 creator A5019216827 @default.
• W2119785014 creator A5024573284 @default.
• W2119785014 creator A5052200795 @default.
• W2119785014 creator A5052948447 @default.
• W2119785014 creator A5074965642 @default.
• W2119785014 creator A5088700318 @default.
• W2119785014 date "2015-11-04" @default.
• W2119785014 modified "2023-10-05" @default.
• W2119785014 title "The A673T mutation in the amyloid precursor protein reduces the production of β-amyloid protein from its β-carboxyl terminal fragment in cells" @default.
• W2119785014 cites W1534001036 @default.
• W2119785014 cites W1577773742 @default.
• W2119785014 cites W1655404681 @default.
• W2119785014 cites W1779570864 @default.
• W2119785014 cites W1963904512 @default.
• W2119785014 cites W1964273058 @default.
• W2119785014 cites W1968995509 @default.
• W2119785014 cites W1969589036 @default.
• W2119785014 cites W1977141816 @default.
• W2119785014 cites W1991435932 @default.
• W2119785014 cites W1993722622 @default.
• W2119785014 cites W2003890494 @default.
• W2119785014 cites W2011424449 @default.
• W2119785014 cites W2024668488 @default.
• W2119785014 cites W2025628875 @default.
• W2119785014 cites W2038771985 @default.
• W2119785014 cites W2040137618 @default.
• W2119785014 cites W2047945039 @default.
• W2119785014 cites W2049451196 @default.
• W2119785014 cites W2055717995 @default.
• W2119785014 cites W2057709960 @default.
• W2119785014 cites W2067772115 @default.
• W2119785014 cites W2076309706 @default.
• W2119785014 cites W2082429191 @default.
• W2119785014 cites W2096410114 @default.
• W2119785014 cites W2097750046 @default.
• W2119785014 cites W2103440561 @default.
• W2119785014 cites W2118330889 @default.
• W2119785014 cites W2119124921 @default.
• W2119785014 cites W2119655548 @default.
• W2119785014 cites W2133019124 @default.
• W2119785014 cites W2144973331 @default.
• W2119785014 cites W2154755614 @default.
• W2119785014 cites W2167354875 @default.
• W2119785014 cites W2169152986 @default.
• W2119785014 doi "https://doi.org/10.1186/s40478-015-0247-6" @default.
• W2119785014 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4632685" @default.
• W2119785014 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26531305" @default.
• W2119785014 hasPublicationYear "2015" @default.
• W2119785014 type Work @default.
• W2119785014 sameAs 2119785014 @default.
• W2119785014 citedByCount "19" @default.
• W2119785014 countsByYear W21197850142016 @default.
• W2119785014 countsByYear W21197850142017 @default.
• W2119785014 countsByYear W21197850142018 @default.
• W2119785014 countsByYear W21197850142019 @default.
• W2119785014 countsByYear W21197850142020 @default.
• W2119785014 countsByYear W21197850142021 @default.
• W2119785014 countsByYear W21197850142022 @default.
• W2119785014 countsByYear W21197850142023 @default.
• W2119785014 crossrefType "journal-article" @default.
• W2119785014 hasAuthorship W2119785014A5016024085 @default.
• W2119785014 hasAuthorship W2119785014A5019216827 @default.
• W2119785014 hasAuthorship W2119785014A5024573284 @default.
• W2119785014 hasAuthorship W2119785014A5052200795 @default.
• W2119785014 hasAuthorship W2119785014A5052948447 @default.
• W2119785014 hasAuthorship W2119785014A5074965642 @default.
• W2119785014 hasAuthorship W2119785014A5088700318 @default.
• W2119785014 hasBestOaLocation W21197850141 @default.
• W2119785014 hasConcept C104317684 @default.
• W2119785014 hasConcept C126322002 @default.
• W2119785014 hasConcept C143065580 @default.
• W2119785014 hasConcept C153911025 @default.
• W2119785014 hasConcept C179104552 @default.
• W2119785014 hasConcept C185592680 @default.
• W2119785014 hasConcept C207583985 @default.
• W2119785014 hasConcept C2777633098 @default.
• W2119785014 hasConcept C2779134260 @default.
• W2119785014 hasConcept C31705614 @default.
• W2119785014 hasConcept C501734568 @default.
• W2119785014 hasConcept C502032728 @default.
• W2119785014 hasConcept C55493867 @default.
• W2119785014 hasConcept C71924100 @default.
• W2119785014 hasConcept C86803240 @default.
• W2119785014 hasConcept C95444343 @default.
• W2119785014 hasConceptScore W2119785014C104317684 @default.
• W2119785014 hasConceptScore W2119785014C126322002 @default.
• W2119785014 hasConceptScore W2119785014C143065580 @default.
• W2119785014 hasConceptScore W2119785014C153911025 @default.
• W2119785014 hasConceptScore W2119785014C179104552 @default.
• W2119785014 hasConceptScore W2119785014C185592680 @default.
• W2119785014 hasConceptScore W2119785014C207583985 @default.
• W2119785014 hasConceptScore W2119785014C2777633098 @default.
• W2119785014 hasConceptScore W2119785014C2779134260 @default.
• W2119785014 hasConceptScore W2119785014C31705614 @default.
• W2119785014 hasConceptScore W2119785014C501734568 @default.
• W2119785014 hasConceptScore W2119785014C502032728 @default.
• W2119785014 hasConceptScore W2119785014C55493867 @default.

• next