FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2119801446> ?p ?o ?g. }

• W2119801446 endingPage "1166" @default.
• W2119801446 startingPage "1159" @default.
• W2119801446 abstract "Genetic code reprogramming allows proteins to sample new chemistry through the defined and targeted introduction of non-natural amino acids (nAAs). Many useful nAAs are derivatives of the natural aromatic amino acid tyrosine, with the para OH group replaced with useful but often bulkier substituents. Extending residue sampling by directed evolution identified positions in Green Fluorescent Protein tolerant to aromatic nAAs, including identification of novel sites that modulate fluorescence. Replacement of the buried L44 residue by photosensitive p-azidophenylalanine (azF) conferred environmentally sensitive photoswitching. In silico modelling of the L44azF dark state provided an insight into the mechanism of action through modulation of the hydrogen bonding network surrounding the chromophore. Targeted mutagenesis of T203 with aromatic nAAs to introduce π-stacking with the chromophore successfully generated red shifted versions of GFP. Incorporation of azF at residue 203 conferred high photosensitivity on sfGFP with even ambient light mediating a functional switch. Thus, engineering proteins with non-natural aromatic amino acids by surveying a wide residue set can introduce new and beneficial properties into a protein through the sampling of non-intuitive mutations. Coupled with retrospective in silico modelling, this will facilitate both our understanding of the impact of nAAs on protein structure and function, and future design endeavours." @default.
• W2119801446 created "2016-06-24" @default.
• W2119801446 creator A5051078643 @default.
• W2119801446 creator A5054423193 @default.
• W2119801446 creator A5058021422 @default.
• W2119801446 creator A5065365203 @default.
• W2119801446 creator A5068655226 @default.
• W2119801446 creator A5068904637 @default.
• W2119801446 date "2015-01-01" @default.
• W2119801446 modified "2023-10-03" @default.
• W2119801446 title "Directed evolution of GFP with non-natural amino acids identifies residues for augmenting and photoswitching fluorescence" @default.
• W2119801446 cites W1789943808 @default.
• W2119801446 cites W1966013120 @default.
• W2119801446 cites W1988326309 @default.
• W2119801446 cites W1998961698 @default.
• W2119801446 cites W1999321313 @default.
• W2119801446 cites W2007893737 @default.
• W2119801446 cites W2008178751 @default.
• W2119801446 cites W2009643752 @default.
• W2119801446 cites W2013745116 @default.
• W2119801446 cites W2026348560 @default.
• W2119801446 cites W2027732943 @default.
• W2119801446 cites W2059602365 @default.
• W2119801446 cites W2062602541 @default.
• W2119801446 cites W2081080113 @default.
• W2119801446 cites W2090212447 @default.
• W2119801446 cites W2093040063 @default.
• W2119801446 cites W2094654556 @default.
• W2119801446 cites W2097985831 @default.
• W2119801446 cites W2099348299 @default.
• W2119801446 cites W2108407770 @default.
• W2119801446 cites W2115202121 @default.
• W2119801446 cites W2115734610 @default.
• W2119801446 cites W2127068865 @default.
• W2119801446 cites W2139469303 @default.
• W2119801446 cites W2145251996 @default.
• W2119801446 cites W2147371334 @default.
• W2119801446 cites W2148313902 @default.
• W2119801446 cites W2150371887 @default.
• W2119801446 cites W2151228364 @default.
• W2119801446 cites W2159723812 @default.
• W2119801446 cites W2161996750 @default.
• W2119801446 cites W2163266294 @default.
• W2119801446 cites W2164788577 @default.
• W2119801446 cites W2169515696 @default.
• W2119801446 cites W2332024781 @default.
• W2119801446 cites W2951758363 @default.
• W2119801446 doi "https://doi.org/10.1039/c4sc02827a" @default.
• W2119801446 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5811120" @default.
• W2119801446 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29560203" @default.
• W2119801446 hasPublicationYear "2015" @default.
• W2119801446 type Work @default.
• W2119801446 sameAs 2119801446 @default.
• W2119801446 citedByCount "23" @default.
• W2119801446 countsByYear W21198014462015 @default.
• W2119801446 countsByYear W21198014462016 @default.
• W2119801446 countsByYear W21198014462017 @default.
• W2119801446 countsByYear W21198014462018 @default.
• W2119801446 countsByYear W21198014462019 @default.
• W2119801446 countsByYear W21198014462020 @default.
• W2119801446 countsByYear W21198014462021 @default.
• W2119801446 countsByYear W21198014462022 @default.
• W2119801446 countsByYear W21198014462023 @default.
• W2119801446 crossrefType "journal-article" @default.
• W2119801446 hasAuthorship W2119801446A5051078643 @default.
• W2119801446 hasAuthorship W2119801446A5054423193 @default.
• W2119801446 hasAuthorship W2119801446A5058021422 @default.
• W2119801446 hasAuthorship W2119801446A5065365203 @default.
• W2119801446 hasAuthorship W2119801446A5068655226 @default.
• W2119801446 hasAuthorship W2119801446A5068904637 @default.
• W2119801446 hasBestOaLocation W21198014461 @default.
• W2119801446 hasConcept C104317684 @default.
• W2119801446 hasConcept C121332964 @default.
• W2119801446 hasConcept C142613039 @default.
• W2119801446 hasConcept C185592680 @default.
• W2119801446 hasConcept C192468462 @default.
• W2119801446 hasConcept C2775905019 @default.
• W2119801446 hasConcept C2777992278 @default.
• W2119801446 hasConcept C2781338088 @default.
• W2119801446 hasConcept C515207424 @default.
• W2119801446 hasConcept C55493867 @default.
• W2119801446 hasConcept C62520636 @default.
• W2119801446 hasConcept C71240020 @default.
• W2119801446 hasConcept C75473681 @default.
• W2119801446 hasConcept C91881484 @default.
• W2119801446 hasConceptScore W2119801446C104317684 @default.
• W2119801446 hasConceptScore W2119801446C121332964 @default.
• W2119801446 hasConceptScore W2119801446C142613039 @default.
• W2119801446 hasConceptScore W2119801446C185592680 @default.
• W2119801446 hasConceptScore W2119801446C192468462 @default.
• W2119801446 hasConceptScore W2119801446C2775905019 @default.
• W2119801446 hasConceptScore W2119801446C2777992278 @default.
• W2119801446 hasConceptScore W2119801446C2781338088 @default.
• W2119801446 hasConceptScore W2119801446C515207424 @default.
• W2119801446 hasConceptScore W2119801446C55493867 @default.
• W2119801446 hasConceptScore W2119801446C62520636 @default.
• W2119801446 hasConceptScore W2119801446C71240020 @default.
• W2119801446 hasConceptScore W2119801446C75473681 @default.

• next