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- W2119890899 abstract "Dry bean (Phaseolus vulgaris L.) is a legume crop generally considered to be a poor N 2 fixer. This experiment was conducted to determine the effectiveness of Rhizobium phaseoli strains in Ontario soils, to determine if inoculation with an indigenous, effective strain could improve N 2 fixation or yield, and to determine the competitiveness of inoculant strains by measuring the recovery of applied strains in nodules using serology. One-gram composite soil samples from 36 of 37 farm sites across Ontario caused nodulation on either white bean cultivars Ex Rico 23 or Seafarer, indicating that R. phaseoli was widely distributed. However, ratings of nodulation indicated a wide range of effectiveness. Five selected strains isolated from these soils were compared with five strains from culture collections for effectiveness by using the strains to inoculate Ex Rico 23 or Seafarer grown in sterile, N-free culture in Leonard jars. The best field-isolated strains caused just as much N accumulation as the best strains from culture collections. Strain S1, selected as a consistently good N 2 fixer, failed to cause increases in yield when used as an inoculant in field trials at Elora and Mitchell in 1980 and 1981. Inoculation with strain S1 did cause some increases in percent seed protein and nodule dry weight with Ex Rico 23 at Elora in 1980. Three other strains tested in each year failed to show any improvements in yield, seed N, or nodulation in either year. Recovery of inoculant strains ranged from 0 to 31%. At Mitchell in 1981, 100 kg ha −1 of fertilizer N increased yields over all inoculation treatments. Indigenous strains appeared to be only partially effective N 2 fixers and inoculant strains generally were poor competitors against strains already present in the soils.Key words: White bean, Phaseolus vulgaris, Rhizobium phaseoli, N 2 fixation, strain competition" @default.
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- W2119890899 date "1986-10-01" @default.
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- W2119890899 title "N2 FIXATION AND COMPETITIVENESS OF Rhizobium phaseoli STRAINS ISOLATED FROM ONTARIO SOILS" @default.
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- W2119890899 doi "https://doi.org/10.4141/cjps86-104" @default.
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