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- W2119942783 abstract "A plate clearing assay was devised to screen for high-producing cellulase mutants of Trichoderma viride . The method employs (i) the use of either rose bengal or oxgall to limit colony size and (ii) Phosfon D (tributyl-2, 4-dichloroben-zylphosphonium chloride) to enhance cellulase detection, in combination with acid-swollen cellulose on agar plates. The method was used to isolate constitutive cellulase mutants of T. viride and should prove useful for isolating high-producing mutants from a range of organisms. This technique has been also used to determine the concentration at which glucose and glycerol inhibit cellulase synthesis by catabolite repression in the wild-type strains." @default.
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- W2119942783 date "1977-01-01" @default.
- W2119942783 modified "2023-09-27" @default.
- W2119942783 title "Semiquantitative Plate Assay for Determination of Cellulase Production by <i>Trichoderma viride</i>" @default.
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- W2119942783 doi "https://doi.org/10.1128/aem.33.1.178-183.1977" @default.
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