Matches in SemOpenAlex for { <https://semopenalex.org/work/W2120080727> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2120080727 abstract "This study was performed to describe the basic methods to isolate and culture of primary satellite cells (PSCs) obtained from 50 to 60-day-old sheep fetuses, single cell cloning of transfected PSCs and sexing of ovine PSCs based on the ZFY/ZFX, amelogenin and high-motility-group (HMG) box sequences.Three-step enzymatic digestion method increased PSCs isolation from tissue and reduced the damage of cells during long time incubation with enzymes. The results of cloning showed that the 103 and 81 clones (from a total of 184 clones) were derived from feeder and bFGF treatment, respectively. The overall sexing efficiency in the present study was 100%. Southern blot results of sex determination were in complete agreement with PCR-amplified bands which confirmed that the HMG box of SRY gene amplified from the ovine genome and that was specific for male.We successfully isolated and cultured sheep primary satellite cells via mechanical and enzymatic disaggregation. Our finding demonstrated that use of feeder and addition of bFGF to the culture medium improved cloning efficiency. The results of sex detection demonstrated that these methods can be applied to detect the sex of primary satellite cells and to determine the sex of sheep embryo prior to produce sheep embryos by somatic cell nuclear transfer technique in vitro. Nevertheless, our findings suggested that sex determination of satellite cells base on amelogenin sequence can be accurate, relatively simple, rapid, and inexpensive." @default.
- W2120080727 created "2016-06-24" @default.
- W2120080727 creator A5004662180 @default.
- W2120080727 creator A5037977093 @default.
- W2120080727 creator A5080356752 @default.
- W2120080727 date "2014-12-01" @default.
- W2120080727 modified "2023-09-27" @default.
- W2120080727 title "Cell culture, sex determination and single cell cloning of ovine transgenic satellite cells in vitro" @default.
- W2120080727 cites W1516384278 @default.
- W2120080727 cites W1538506308 @default.
- W2120080727 cites W1973567527 @default.
- W2120080727 cites W1981844839 @default.
- W2120080727 cites W1987999485 @default.
- W2120080727 cites W2011467790 @default.
- W2120080727 cites W2016573112 @default.
- W2120080727 cites W2022126715 @default.
- W2120080727 cites W2023548437 @default.
- W2120080727 cites W2047170549 @default.
- W2120080727 cites W2077034159 @default.
- W2120080727 cites W2092077225 @default.
- W2120080727 cites W2143951314 @default.
- W2120080727 cites W2148502461 @default.
- W2120080727 cites W2158272906 @default.
- W2120080727 cites W2163424015 @default.
- W2120080727 cites W2323322496 @default.
- W2120080727 cites W2348950409 @default.
- W2120080727 doi "https://doi.org/10.1186/s40709-014-0022-z" @default.
- W2120080727 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4390094" @default.
- W2120080727 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25984504" @default.
- W2120080727 hasPublicationYear "2014" @default.
- W2120080727 type Work @default.
- W2120080727 sameAs 2120080727 @default.
- W2120080727 citedByCount "2" @default.
- W2120080727 countsByYear W21200807272017 @default.
- W2120080727 crossrefType "journal-article" @default.
- W2120080727 hasAuthorship W2120080727A5004662180 @default.
- W2120080727 hasAuthorship W2120080727A5037977093 @default.
- W2120080727 hasAuthorship W2120080727A5080356752 @default.
- W2120080727 hasBestOaLocation W21200807271 @default.
- W2120080727 hasConcept C102230213 @default.
- W2120080727 hasConcept C104317684 @default.
- W2120080727 hasConcept C106434640 @default.
- W2120080727 hasConcept C121050878 @default.
- W2120080727 hasConcept C134305767 @default.
- W2120080727 hasConcept C1491633281 @default.
- W2120080727 hasConcept C153911025 @default.
- W2120080727 hasConcept C196843134 @default.
- W2120080727 hasConcept C199360897 @default.
- W2120080727 hasConcept C2779383911 @default.
- W2120080727 hasConcept C41008148 @default.
- W2120080727 hasConcept C54009773 @default.
- W2120080727 hasConcept C54355233 @default.
- W2120080727 hasConcept C81885089 @default.
- W2120080727 hasConcept C86803240 @default.
- W2120080727 hasConcept C95444343 @default.
- W2120080727 hasConceptScore W2120080727C102230213 @default.
- W2120080727 hasConceptScore W2120080727C104317684 @default.
- W2120080727 hasConceptScore W2120080727C106434640 @default.
- W2120080727 hasConceptScore W2120080727C121050878 @default.
- W2120080727 hasConceptScore W2120080727C134305767 @default.
- W2120080727 hasConceptScore W2120080727C1491633281 @default.
- W2120080727 hasConceptScore W2120080727C153911025 @default.
- W2120080727 hasConceptScore W2120080727C196843134 @default.
- W2120080727 hasConceptScore W2120080727C199360897 @default.
- W2120080727 hasConceptScore W2120080727C2779383911 @default.
- W2120080727 hasConceptScore W2120080727C41008148 @default.
- W2120080727 hasConceptScore W2120080727C54009773 @default.
- W2120080727 hasConceptScore W2120080727C54355233 @default.
- W2120080727 hasConceptScore W2120080727C81885089 @default.
- W2120080727 hasConceptScore W2120080727C86803240 @default.
- W2120080727 hasConceptScore W2120080727C95444343 @default.
- W2120080727 hasIssue "1" @default.
- W2120080727 hasLocation W21200807271 @default.
- W2120080727 hasLocation W21200807272 @default.
- W2120080727 hasLocation W21200807273 @default.
- W2120080727 hasLocation W21200807274 @default.
- W2120080727 hasOpenAccess W2120080727 @default.
- W2120080727 hasPrimaryLocation W21200807271 @default.
- W2120080727 hasRelatedWork W1148639210 @default.
- W2120080727 hasRelatedWork W1587200149 @default.
- W2120080727 hasRelatedWork W2347120132 @default.
- W2120080727 hasRelatedWork W2348456236 @default.
- W2120080727 hasRelatedWork W2348538698 @default.
- W2120080727 hasRelatedWork W2350699797 @default.
- W2120080727 hasRelatedWork W2374858880 @default.
- W2120080727 hasRelatedWork W2384261171 @default.
- W2120080727 hasRelatedWork W2389697466 @default.
- W2120080727 hasRelatedWork W3201480737 @default.
- W2120080727 hasVolume "21" @default.
- W2120080727 isParatext "false" @default.
- W2120080727 isRetracted "false" @default.
- W2120080727 magId "2120080727" @default.
- W2120080727 workType "article" @default.