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- W2120147425 abstract "In order to fast and economically purify MTGase from Streptoverticillium ladakanum, a stepwise elution method was developed and compared with linear gradient elution method. MTGase was purified to electrophoretical homogeneity by using CM Sepharose CL-6B and Blue Sepharose Fast Flow chromatographies by linear gradient or stepwise methods. The recovery of MTGase by linear gradient and stepwise methods were 68.4% and 81.0%, respectively. The optimal temperature and pH were 40 degrees C and 5.5, respectively. It was stable at pH 5.0 to 7.0 and had a rate constant (K(D)) of 6.21 x 10(-5) min-1 for thermal inactivation at 45 degrees C. The purified MTGase was activated by K+, Na+, Ca2+, Mn2+, and Mg2+, not affected by Fe3+, EDTA, but inhibited by Cu2+, Zn2+, Hg2+, Ni2+, Co2+, Cd2+, PCMB, NEM, IAA, and PMSF. A simple stepwise method was developed for the purification of MTGase from S. ladakanum." @default.
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- W2120147425 date "2000-01-01" @default.
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- W2120147425 title "Technical Approach to Simplify the Purification Method and Characterization of Microbial Transglutaminase Produced from Streptoverticillium ladakanum" @default.
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- W2120147425 doi "https://doi.org/10.1111/j.1365-2621.2000.tb15959.x" @default.
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