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• W2120147786 abstract "Multiple displacement amplification (MDA) is a technique used in the amplification of very low amounts of DNA and reported to yield large quantities of high-quality DNA. We used MDA to amplify the whole genome directly from a single cell. The most common techniques used in PGD are PCR and fluorescent in-situ hybridization (FISH). There are many limitations to these techniques including, the number of chromosomes diagnosed for FISH or the quality of DNA issued from a single cell PCR. This report shows, for the first time, use of MDA for single cell whole genome amplification. A total of 16 short tandem repeats (STRs) were amplified successfully with a similar pattern to the genomic DNA. Furthermore, allelic drop out (ADO) derived from MDA was assessed in 40 single cells by analysing (i) heterozygosity for a known β globin mutation (IVSI-5 C–G) and by studying (ii) the heterozygous loci present in the STRs. ADO turned out to be 10.25% for the β globin gene sequencing and 5% for the fluorescent PCR analysis of STRs. Moreover, the amplification accuracy of MDA permitted the detection of trisomy 21 on a single cell using comparative genome hybridization-array. Altogether, these data suggest that MDA can be used for single cell molecular karyotyping and the diagnosis of any single gene disorder in PGD." @default.
• W2120147786 created "2016-06-24" @default.
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• W2120147786 date "2004-11-01" @default.
• W2120147786 modified "2023-10-11" @default.
• W2120147786 title "Multiple displacement amplification on single cell and possible PGD applications" @default.
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• W2120147786 doi "https://doi.org/10.1093/molehr/gah114" @default.
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