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- W212028897 abstract "There have been reports that the level of abscisic acid (ABA) increases during the cold storage of tomatoes. However, the important ABA metabolites, phaseic acid (PA) and dihydrophaseic acid (DPA) were never quantitatively determined in such a system. In order to obtain the labeled standards for quantitative determination of those compounds by GC-MS-SIM, we fed bean plants with 6,6,6-({sup 2}H{sub 3})-ABA (mean isotopic enrichment 60%) with addition of about 10{sup 5} Bq per mg of ({sup 3}H)-ABA. After 100 hours the plants were harvested and extracted with acetone. The extract were purified by solvent partitioning and, Prep-Sep amino column and on an HPLC C{sub 18} reverse phase column. Two major radioactive metabolites of ABA were obtained and identified by GC-MS as PA and DPA. Some results on the quantitation of ABA, PA and DPA in tomato fruit after cold storage will be presented." @default.
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- W212028897 date "1990-05-01" @default.
- W212028897 modified "2023-09-24" @default.
- W212028897 title "Preparation of sup 2 H- and sup 3 H-labeled phaseic acid and dihydrophaseic acid as standards for determination of abscisic acid metabolites in tomato fruit" @default.
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