FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2120398709> ?p ?o ?g. }

• W2120398709 endingPage "1431" @default.
• W2120398709 startingPage "1427" @default.
• W2120398709 abstract "We determined the Michaelis constant ( K m ) and maximal velocity ( V max ) of lactate dehydrogenase (LDH) in periportal hepatocytes and skeletal muscle fibers by three different histochemical assay methods. Unfixed sections of mouse liver and gastrocnemius were incubated at 37C either on substrate (l-lactate)-containing agarose gel films or in aqueous assay media with and without 18% polyvinyl alcohol (PVA) as a tissue protectant. The absorbances of the formazan final reaction products were continuously measured at 584 nm in the cytoplasm of individual cells as a function of incubation time, using an image analysis system. The kinetic parameters of purified rabbit skeletal muscle LDH incorporated into polyacrylamide gel sections were similarly determined. The intrinsic initial velocities ( v i ) of LDH, corrected for “nothing dehydrogenase,” were determined as described in the previous article. The K m and V max were calculated from Hanes plots of s/ vi on l-lactate concentration ( s). The K m values obtained with three assay methods were similar and in the range of 21.1–21.9 mM for pure LDH, 8.62–13.5 mM for LDH in mouse periportal hepatocytes, and 13.3–17.9 mM for LDH in mouse skeletal muscle fibers. The V max values determined on agarose gel substrate films and in aqueous assay media without PVA were in good agreeement but were 53–65% lower when 18% PVA was included in the medium. These results indicate that catalytic center activity k cat of LDH is retarded by the high viscosity of PVA media because PVA hardly inhibited the enzyme. The K m values of LDH determined histochemically in skeletal muscle fibers and periportal hepatocytes were respectively three to five times and two to three times higher than those determined biochemically. These differences may be due to interactions of LDH with intracellular components." @default.
• W2120398709 created "2016-06-24" @default.
• W2120398709 creator A5033053999 @default.
• W2120398709 creator A5091676136 @default.
• W2120398709 date "1997-10-01" @default.
• W2120398709 modified "2023-10-17" @default.
• W2120398709 title "Kinetic Parameters of Lactate Dehydrogenase in Liver and Gastrocnemius Determined by Three Quantitative Histochemical Methods" @default.
• W2120398709 cites W107668972 @default.
• W2120398709 cites W1520573090 @default.
• W2120398709 cites W1551848857 @default.
• W2120398709 cites W1565030246 @default.
• W2120398709 cites W1573196340 @default.
• W2120398709 cites W1983971658 @default.
• W2120398709 cites W1989544026 @default.
• W2120398709 cites W1993398265 @default.
• W2120398709 cites W1993990609 @default.
• W2120398709 cites W2017921679 @default.
• W2120398709 cites W2024373836 @default.
• W2120398709 cites W2025874321 @default.
• W2120398709 cites W2035159520 @default.
• W2120398709 cites W2037250176 @default.
• W2120398709 cites W2047389920 @default.
• W2120398709 cites W2061984624 @default.
• W2120398709 cites W2065600150 @default.
• W2120398709 cites W2066623833 @default.
• W2120398709 cites W2069653077 @default.
• W2120398709 cites W2072113326 @default.
• W2120398709 cites W2073052118 @default.
• W2120398709 cites W2073542556 @default.
• W2120398709 cites W2073863107 @default.
• W2120398709 cites W2103529603 @default.
• W2120398709 cites W2129909236 @default.
• W2120398709 cites W2436271364 @default.
• W2120398709 cites W4620627 @default.
• W2120398709 cites W6299193 @default.
• W2120398709 doi "https://doi.org/10.1177/002215549704501011" @default.
• W2120398709 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9313804" @default.
• W2120398709 hasPublicationYear "1997" @default.
• W2120398709 type Work @default.
• W2120398709 sameAs 2120398709 @default.
• W2120398709 citedByCount "10" @default.
• W2120398709 countsByYear W21203987092013 @default.
• W2120398709 countsByYear W21203987092019 @default.
• W2120398709 countsByYear W21203987092020 @default.
• W2120398709 countsByYear W21203987092022 @default.
• W2120398709 crossrefType "journal-article" @default.
• W2120398709 hasAuthorship W2120398709A5033053999 @default.
• W2120398709 hasAuthorship W2120398709A5091676136 @default.
• W2120398709 hasBestOaLocation W21203987091 @default.
• W2120398709 hasConcept C105702510 @default.
• W2120398709 hasConcept C153911025 @default.
• W2120398709 hasConcept C178790620 @default.
• W2120398709 hasConcept C181199279 @default.
• W2120398709 hasConcept C184651966 @default.
• W2120398709 hasConcept C185592680 @default.
• W2120398709 hasConcept C18903297 @default.
• W2120398709 hasConcept C25642318 @default.
• W2120398709 hasConcept C2776317432 @default.
• W2120398709 hasConcept C2776892249 @default.
• W2120398709 hasConcept C2777289219 @default.
• W2120398709 hasConcept C2777318727 @default.
• W2120398709 hasConcept C2778476535 @default.
• W2120398709 hasConcept C2779959927 @default.
• W2120398709 hasConcept C43617362 @default.
• W2120398709 hasConcept C55493867 @default.
• W2120398709 hasConcept C86803240 @default.
• W2120398709 hasConceptScore W2120398709C105702510 @default.
• W2120398709 hasConceptScore W2120398709C153911025 @default.
• W2120398709 hasConceptScore W2120398709C178790620 @default.
• W2120398709 hasConceptScore W2120398709C181199279 @default.
• W2120398709 hasConceptScore W2120398709C184651966 @default.
• W2120398709 hasConceptScore W2120398709C185592680 @default.
• W2120398709 hasConceptScore W2120398709C18903297 @default.
• W2120398709 hasConceptScore W2120398709C25642318 @default.
• W2120398709 hasConceptScore W2120398709C2776317432 @default.
• W2120398709 hasConceptScore W2120398709C2776892249 @default.
• W2120398709 hasConceptScore W2120398709C2777289219 @default.
• W2120398709 hasConceptScore W2120398709C2777318727 @default.
• W2120398709 hasConceptScore W2120398709C2778476535 @default.
• W2120398709 hasConceptScore W2120398709C2779959927 @default.
• W2120398709 hasConceptScore W2120398709C43617362 @default.
• W2120398709 hasConceptScore W2120398709C55493867 @default.
• W2120398709 hasConceptScore W2120398709C86803240 @default.
• W2120398709 hasIssue "10" @default.
• W2120398709 hasLocation W21203987091 @default.
• W2120398709 hasLocation W21203987092 @default.
• W2120398709 hasOpenAccess W2120398709 @default.
• W2120398709 hasPrimaryLocation W21203987091 @default.
• W2120398709 hasRelatedWork W1597097958 @default.
• W2120398709 hasRelatedWork W1962318030 @default.
• W2120398709 hasRelatedWork W1981216115 @default.
• W2120398709 hasRelatedWork W2019304997 @default.
• W2120398709 hasRelatedWork W2031115533 @default.
• W2120398709 hasRelatedWork W2036923792 @default.
• W2120398709 hasRelatedWork W2103529603 @default.
• W2120398709 hasRelatedWork W2405803346 @default.
• W2120398709 hasRelatedWork W2409017684 @default.
• W2120398709 hasRelatedWork W2092027940 @default.

• next