Matches in SemOpenAlex for { <https://semopenalex.org/work/W2120684498> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2120684498 endingPage "2067" @default.
- W2120684498 startingPage "2059" @default.
- W2120684498 abstract "Oncogenic mutation of nuclear transcription factors often is associated with altered patterns of subcellular localization that may be of functional importance. The leukemogenic transcription factor gene E2A-PBX1 is created through fusion of the genes E2A and PBX1 as a result of t(1;19) in acute lymphoblastic leukemia. We evaluated subcellular localization patterns of E2A-PBX1 protein in transfected cells using immunofluorescence. Full-length E2A-PBX1 was exclusively nuclear and was concentrated in spherical domains denoted chimeric-E2A oncoprotein domains (CODs). In contrast, nuclear fluorescence for wild-type E2A or PBX1 proteins was diffuse. Enhanced concentrations of RNA polymerase II within many CODs and the requirement for an E2A-encoded activation domain suggested transcriptional relevance. However, in situ co-detection of nascent transcripts labeled with bromouridine failed to confirm altered transcriptional activity in relation to CODs. CODs also failed to co-localize with other proteins known to occupy functional nuclear compartments, including the transcription factor PML, the spliceosome-associated protein SC-35 and the adenovirus replication factor DBP, or with foci of DNA replication. Co-transfection of Hoxb7, a homeodomain protein capable of enhancing DNA binding by PBX1, impaired COD formation, suggesting that CODs contain E2A-PBX1 protein not associated with DNA. We conclude that, as a 'gain of function' phenomenon requiring protein elements from both E2A and PBX1, COD formation may be relevant to the biology of E2A-PBX1 in leukemogenesis." @default.
- W2120684498 created "2016-06-24" @default.
- W2120684498 creator A5024852195 @default.
- W2120684498 creator A5029486721 @default.
- W2120684498 creator A5059500848 @default.
- W2120684498 creator A5069969724 @default.
- W2120684498 date "1997-10-23" @default.
- W2120684498 modified "2023-10-18" @default.
- W2120684498 title "The chimeric oncoproteins E2A-PBX1 and E2A-HLF are concentrated within spherical nuclear domains" @default.
- W2120684498 doi "https://doi.org/10.1038/sj.onc.1201367" @default.
- W2120684498 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9366523" @default.
- W2120684498 hasPublicationYear "1997" @default.
- W2120684498 type Work @default.
- W2120684498 sameAs 2120684498 @default.
- W2120684498 citedByCount "14" @default.
- W2120684498 countsByYear W21206844982012 @default.
- W2120684498 countsByYear W21206844982015 @default.
- W2120684498 countsByYear W21206844982021 @default.
- W2120684498 crossrefType "journal-article" @default.
- W2120684498 hasAuthorship W2120684498A5024852195 @default.
- W2120684498 hasAuthorship W2120684498A5029486721 @default.
- W2120684498 hasAuthorship W2120684498A5059500848 @default.
- W2120684498 hasAuthorship W2120684498A5069969724 @default.
- W2120684498 hasBestOaLocation W21206844981 @default.
- W2120684498 hasConcept C104317684 @default.
- W2120684498 hasConcept C123894998 @default.
- W2120684498 hasConcept C138885662 @default.
- W2120684498 hasConcept C153911025 @default.
- W2120684498 hasConcept C179926584 @default.
- W2120684498 hasConcept C29512474 @default.
- W2120684498 hasConcept C33987129 @default.
- W2120684498 hasConcept C40767141 @default.
- W2120684498 hasConcept C41895202 @default.
- W2120684498 hasConcept C49805395 @default.
- W2120684498 hasConcept C54009773 @default.
- W2120684498 hasConcept C54355233 @default.
- W2120684498 hasConcept C552990157 @default.
- W2120684498 hasConcept C73573662 @default.
- W2120684498 hasConcept C86339819 @default.
- W2120684498 hasConcept C86803240 @default.
- W2120684498 hasConcept C95444343 @default.
- W2120684498 hasConceptScore W2120684498C104317684 @default.
- W2120684498 hasConceptScore W2120684498C123894998 @default.
- W2120684498 hasConceptScore W2120684498C138885662 @default.
- W2120684498 hasConceptScore W2120684498C153911025 @default.
- W2120684498 hasConceptScore W2120684498C179926584 @default.
- W2120684498 hasConceptScore W2120684498C29512474 @default.
- W2120684498 hasConceptScore W2120684498C33987129 @default.
- W2120684498 hasConceptScore W2120684498C40767141 @default.
- W2120684498 hasConceptScore W2120684498C41895202 @default.
- W2120684498 hasConceptScore W2120684498C49805395 @default.
- W2120684498 hasConceptScore W2120684498C54009773 @default.
- W2120684498 hasConceptScore W2120684498C54355233 @default.
- W2120684498 hasConceptScore W2120684498C552990157 @default.
- W2120684498 hasConceptScore W2120684498C73573662 @default.
- W2120684498 hasConceptScore W2120684498C86339819 @default.
- W2120684498 hasConceptScore W2120684498C86803240 @default.
- W2120684498 hasConceptScore W2120684498C95444343 @default.
- W2120684498 hasIssue "17" @default.
- W2120684498 hasLocation W21206844981 @default.
- W2120684498 hasLocation W21206844982 @default.
- W2120684498 hasOpenAccess W2120684498 @default.
- W2120684498 hasPrimaryLocation W21206844981 @default.
- W2120684498 hasRelatedWork W2008897992 @default.
- W2120684498 hasRelatedWork W2012831646 @default.
- W2120684498 hasRelatedWork W2018096124 @default.
- W2120684498 hasRelatedWork W2149093922 @default.
- W2120684498 hasRelatedWork W2162684571 @default.
- W2120684498 hasRelatedWork W2163747968 @default.
- W2120684498 hasRelatedWork W2164850454 @default.
- W2120684498 hasRelatedWork W2382432990 @default.
- W2120684498 hasRelatedWork W2587803312 @default.
- W2120684498 hasRelatedWork W3120216242 @default.
- W2120684498 hasVolume "15" @default.
- W2120684498 isParatext "false" @default.
- W2120684498 isRetracted "false" @default.
- W2120684498 magId "2120684498" @default.
- W2120684498 workType "article" @default.