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• W2120791736 abstract "The essential Nps1p/Sth1p is a catalytic subunit of the nucleosome‐remodeling complex, RSC, of Saccharomyces cerevisiae that can alter nucleosome structure by using the energy of ATP hydrolysis. Besides the ATPase domain, Nps1p harbors the bromodomain, of which the function(s) have not yet been defined. We have isolated a temperature‐sensitive mutant allele of NPS1 , nps1‐13 , which has amino acid substitutions within the bromodomain. This mutation perturbed the interaction between the RSC components and enhanced the sensitivity of the cells to several DNA‐damaging treatments at the permissive temperature. Reduced expression of NPS1 also caused DNA damage sensitivity. These results suggest the importance of the Nps1p bromodomain in RSC integrity and a model in which high amounts of RSC would be required for the cells to overcome DNA damage." @default.
• W2120791736 created "2016-06-24" @default.
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• W2120791736 date "2002-10-05" @default.
• W2120791736 modified "2023-10-09" @default.
• W2120791736 title "Abundance of the RSC nucleosome‐remodeling complex is important for the cells to tolerate DNA damage in <i>Saccharomyces cerevisiae</i>" @default.
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• W2120791736 doi "https://doi.org/10.1016/s0014-5793(02)03504-4" @default.
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• W2120791736 hasPublicationYear "2002" @default.
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