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- W2120792044 abstract "The toxicity and metabolism of 2,4-dichlorophenol, with regard to the aquatic macrophyte Lemna gibba (duckweed), have been studied Toxicity is described in terms of the effect of 2,4-dichlorophenol (2,4-DCP) on the vegetative reproduction of duckweed over a 10-d growth period, the EC10 and EC50 were 2.5 and 9 2 μM, respectively. Metabolism of 2,4-dichlorophenol was monitored by incubation of the plants with radiolabeled substrate, and periodic sampling and analysis by reversed-phase HPLC of the plant growth medium Depending on the growth conditions, up to 95% of the 2,4-DCP was metabolized over a 6-d growth period. To analyze the metabolites, the plants were grown in the presence of sublethal concentrations of [U-14C]-2,4-DCP. The growth medium was lyophihzed and then mixed with the plants, extracted, and analyzed using reversed-phase HPLC, followed by scintillation counting of the fractions The major metabolite was isolated and identified as 2,4-dichlo-rophenyl-β-D-glucopyranoside by high-field NMR and MS. The structure of the metabolite was confirmed by synthesis and by enzymatic cleavage of the β-glucosidic linkage to afford 2,4-DCP. An important consequence of conjugate formation is the masking of the presence of 2,4-DCP to the usual analytical techniques used for its detection and quantitation. This finding is probably applicable to other contaminants and organisms." @default.
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- W2120792044 date "1994-02-01" @default.
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- W2120792044 title "Toxicity and metabolism of 2,4-dichlorophenol by the aquatic angiosperm<i>Lemna gibba</i>" @default.
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- W2120792044 doi "https://doi.org/10.1002/etc.5620130217" @default.
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