FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2120870359> ?p ?o ?g. }

• W2120870359 abstract "Intersubtype HIV-1 recombinants in the form of unique or stable circulating recombinants forms (CRFs) are responsible for over 20% of infections in the worldwide epidemic. Mechanisms controlling the generation, selection, and transmission of these intersubtype HIV-1 recombinants still require further investigation. All intersubtype HIV-1 recombinants are generated and evolve from initial dual infections, but are difficult to identify in the human population. In vitro studies provide the most practical system to study mechanisms, but the recombination rates are usually very low in dual infections with primary HIV-1 isolates. This study describes the use of HIV-1 isolate-specific siRNAs to enrich intersubtype HIV-1 recombinants and inhibit the parental HIV-1 isolates from a dual infection. Following a dual infection with subtype A and D primary HIV-1 isolates and two rounds of siRNA treatment, nearly 100% of replicative virus was resistant to a siRNA specific for an upstream target sequence in the subtype A envelope (env) gene as well as a siRNA specific for a downstream target sequence in the subtype D env gene. Only 20% (10/50) of the replicating virus had nucleotide substitutions in the siRNA-target sequence whereas the remaining 78% (39/50) harbored a recombination breakpoint that removed both siRNA target sequences, and rendered the intersubtype D/A recombinant virus resistant to the dual siRNA treatment. Since siRNAs target the newly transcribed HIV-1 mRNA, the siRNAs only enrich intersubtype env recombinants and do not influence the recombination process during reverse transcription. Using this system, a strong bias is selected for recombination breakpoints in the C2 region, whereas other HIV-1 env regions, most notably the hypervariable regions, were nearly devoid of intersubtype recombination breakpoints. Sequence conservation plays an important role in selecting for recombination breakpoints, but the lack of breakpoints in many conserved env regions suggest that other mechanisms are at play. These findings show that siRNAs can be used as an efficient in vitro tool for enriching recombinants, to facilitate further study on mechanisms of intersubytpe HIV-1 recombination, and to generate replication-competent intersubtype recombinant proteins with a breadth in HIV-1 diversity for future vaccine studies." @default.
• W2120870359 created "2016-06-24" @default.
• W2120870359 creator A5014190782 @default.
• W2120870359 creator A5018319496 @default.
• W2120870359 creator A5024658613 @default.
• W2120870359 creator A5043481939 @default.
• W2120870359 creator A5063226548 @default.
• W2120870359 creator A5074401742 @default.
• W2120870359 creator A5076033998 @default.
• W2120870359 date "2011-01-13" @default.
• W2120870359 modified "2023-09-27" @default.
• W2120870359 title "Enrichment of intersubtype HIV-1 recombinants in a dual infection system using HIV-1 strain-specific siRNAs" @default.
• W2120870359 cites W144423133 @default.
• W2120870359 cites W1488192195 @default.
• W2120870359 cites W1647075334 @default.
• W2120870359 cites W1966771136 @default.
• W2120870359 cites W1966800969 @default.
• W2120870359 cites W1970301294 @default.
• W2120870359 cites W1970335365 @default.
• W2120870359 cites W1974566789 @default.
• W2120870359 cites W1984588952 @default.
• W2120870359 cites W1996203890 @default.
• W2120870359 cites W2026793457 @default.
• W2120870359 cites W2037979460 @default.
• W2120870359 cites W2054239484 @default.
• W2120870359 cites W2060534902 @default.
• W2120870359 cites W2073004681 @default.
• W2120870359 cites W2087393491 @default.
• W2120870359 cites W2088349342 @default.
• W2120870359 cites W2092727091 @default.
• W2120870359 cites W2097382368 @default.
• W2120870359 cites W2098524212 @default.
• W2120870359 cites W2099219682 @default.
• W2120870359 cites W2101350687 @default.
• W2120870359 cites W2103262528 @default.
• W2120870359 cites W2112421483 @default.
• W2120870359 cites W2117991391 @default.
• W2120870359 cites W2122107943 @default.
• W2120870359 cites W2133541971 @default.
• W2120870359 cites W2136498216 @default.
• W2120870359 cites W2137060574 @default.
• W2120870359 cites W2140091023 @default.
• W2120870359 cites W2142401625 @default.
• W2120870359 cites W2147413871 @default.
• W2120870359 cites W2151856127 @default.
• W2120870359 cites W2166903671 @default.
• W2120870359 cites W2166915947 @default.
• W2120870359 cites W2256756668 @default.
• W2120870359 cites W4254038279 @default.
• W2120870359 doi "https://doi.org/10.1186/1742-4690-8-5" @default.
• W2120870359 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3025951" @default.
• W2120870359 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21232148" @default.
• W2120870359 hasPublicationYear "2011" @default.
• W2120870359 type Work @default.
• W2120870359 sameAs 2120870359 @default.
• W2120870359 citedByCount "7" @default.
• W2120870359 countsByYear W21208703592012 @default.
• W2120870359 countsByYear W21208703592013 @default.
• W2120870359 countsByYear W21208703592014 @default.
• W2120870359 countsByYear W21208703592015 @default.
• W2120870359 countsByYear W21208703592017 @default.
• W2120870359 crossrefType "journal-article" @default.
• W2120870359 hasAuthorship W2120870359A5014190782 @default.
• W2120870359 hasAuthorship W2120870359A5018319496 @default.
• W2120870359 hasAuthorship W2120870359A5024658613 @default.
• W2120870359 hasAuthorship W2120870359A5043481939 @default.
• W2120870359 hasAuthorship W2120870359A5063226548 @default.
• W2120870359 hasAuthorship W2120870359A5074401742 @default.
• W2120870359 hasAuthorship W2120870359A5076033998 @default.
• W2120870359 hasBestOaLocation W21208703591 @default.
• W2120870359 hasConcept C104317684 @default.
• W2120870359 hasConcept C156695909 @default.
• W2120870359 hasConcept C156719811 @default.
• W2120870359 hasConcept C159047783 @default.
• W2120870359 hasConcept C2522874641 @default.
• W2120870359 hasConcept C2779690655 @default.
• W2120870359 hasConcept C2780727368 @default.
• W2120870359 hasConcept C2908647359 @default.
• W2120870359 hasConcept C54355233 @default.
• W2120870359 hasConcept C67705224 @default.
• W2120870359 hasConcept C71924100 @default.
• W2120870359 hasConcept C86803240 @default.
• W2120870359 hasConcept C99454951 @default.
• W2120870359 hasConceptScore W2120870359C104317684 @default.
• W2120870359 hasConceptScore W2120870359C156695909 @default.
• W2120870359 hasConceptScore W2120870359C156719811 @default.
• W2120870359 hasConceptScore W2120870359C159047783 @default.
• W2120870359 hasConceptScore W2120870359C2522874641 @default.
• W2120870359 hasConceptScore W2120870359C2779690655 @default.
• W2120870359 hasConceptScore W2120870359C2780727368 @default.
• W2120870359 hasConceptScore W2120870359C2908647359 @default.
• W2120870359 hasConceptScore W2120870359C54355233 @default.
• W2120870359 hasConceptScore W2120870359C67705224 @default.
• W2120870359 hasConceptScore W2120870359C71924100 @default.
• W2120870359 hasConceptScore W2120870359C86803240 @default.
• W2120870359 hasConceptScore W2120870359C99454951 @default.
• W2120870359 hasIssue "1" @default.
• W2120870359 hasLocation W21208703591 @default.
• W2120870359 hasLocation W21208703592 @default.
• W2120870359 hasLocation W21208703593 @default.

• next