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• W2120876680 startingPage "3574" @default.
• W2120876680 abstract "ABSTRACT Hypothetically, since native HIV-1 Env trimers are exclusively recognized by neutralizing antibodies, they might induce the neutralizing antibodies in a vaccine setting. This idea has not been evaluated due to the difficulty of separating trimers from nonfunctional Env (uncleaved gp160 and gp41 stumps). The latter are immunodominant and induce nonneutralizing antibodies. We previously showed that nonfunctional Env can be selectively cleared from virus-like particle (VLP) surfaces by enzyme digests (E. T. Crooks, T. Tong , K. Osawa, and J. M. Binley, J.Virol. 85:5825, 2011). Here, we investigated the effects of these digests on the antigenicity of VLPs and their sensitivity to neutralization. Before digestion, WT VLPs (bearing wild-type Env) and UNC VLPs (bearing uncleaved gp160) were recognized by various Env-specific monoclonal antibodies (MAbs), irrespective of their neutralizing activity, a result which is consistent with the presence of nonfunctional Env. After digestion, only neutralizing MAbs recognized WT VLPs, consistent with selective removal of nonfunctional Env (i.e., “trimer VLPs”). Digests eliminated the binding of all MAbs to UNC VLPs, again consistent with removal of nonfunctional Env. An exception was MAb 2F5, which weakly bound to digested UNC VLPs and bald VLPs (bearing no Env), perhaps due to lipid cross-reactivity. Trimer VLPs were infectious, and their neutralization sensitivity was largely comparable to that of undigested WT VLPs. However, they were ∼100-fold more sensitive to the MAbs 4E10 and Z13e1, suggesting increased exposure of the gp41 base. Importantly, a scatterplot analysis revealed a strong correlation between MAb binding and neutralization of trimer VLPs. This suggests that trimer VLPs bear essentially pure native trimer that should allow its unfettered evaluation in a vaccine setting." @default.
• W2120876680 created "2016-06-24" @default.
• W2120876680 creator A5023994515 @default.
• W2120876680 creator A5027151578 @default.
• W2120876680 creator A5036810091 @default.
• W2120876680 creator A5069640061 @default.
• W2120876680 date "2012-04-01" @default.
• W2120876680 modified "2023-10-11" @default.
• W2120876680 title "HIV-1 Virus-Like Particles Bearing Pure Env Trimers Expose Neutralizing Epitopes but Occlude Nonneutralizing Epitopes" @default.
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• W2120876680 doi "https://doi.org/10.1128/jvi.06938-11" @default.
• W2120876680 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3302546" @default.
• W2120876680 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22301141" @default.
• W2120876680 hasPublicationYear "2012" @default.
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