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• W2120878319 abstract "Human metapneumovirus (hMPV) is a recently described paramyxovirus that has been associated with acute upper and lower respiratory infection (LRI) in infants and children worldwide. We previously observed that one-third of the children with hMPV-associated LRI had been diagnosed with a concomitant acute otitis media (AOM). In the current study, we sought to investigate an association between hMPV and children presenting with AOM as a primary diagnosis. We used realtime RT-PCR for hMPV to retrospectively test 144 paired nasal wash (NW) and middle ear fluid (MEF) specimens that had been prospectively collected from children with AOM during a 3-year period from 1990–1992. RNA was extracted from archived, frozen samples and realtime RT-PCR for hMPV was performed. We detected hMPV in 8/144 (6%) NW and 1/144 MEF. Several of the children still tested positive for hMPV in NW 3 days later, showing persistent virus shedding. All were detected from November–May and six had bacterial co-pathogens. Two of the eight (25%) hMPV-infected children had no bacterial pathogen isolated, suggesting that hMPV may be associated with AOM as a sole pathogen. These data show that hMPV is associated with a proportion of AOM and thus has additional morbidity and healthcare impact related to these illnesses." @default.
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• W2120878319 date "2006-07-01" @default.
• W2120878319 modified "2023-10-15" @default.
• W2120878319 title "Association of human metapneumovirus with acute otitis media" @default.
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• W2120878319 doi "https://doi.org/10.1016/j.ijporl.2005.12.007" @default.
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