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- W2120959357 endingPage "1686" @default.
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- W2120959357 abstract "Hormones integrate the activities of their target cells through receptor-modulated cascades of protein interactions that ultimately lead to changes in cellular function. Understanding how the cell assembles these signaling protein complexes is critically important to unraveling disease processes, and to the design of therapeutic strategies. Recent advances in live-cell imaging technologies, combined with the use of genetically encoded fluorescent proteins, now allow the assembly of these signaling protein complexes to be tracked within the organized microenvironment of the living cell. Here, we review some of the recent developments in the application of imaging techniques to measure the dynamic behavior, colocalization, and spatial relationships between proteins in living cells. Where possible, we discuss the application of these different approaches in the context of hormone regulation of nuclear receptor localization, mobility, and interactions in different subcellular compartments. We discuss measurements that define the spatial relationships and dynamics between proteins in living cells including fluorescence colocalization, fluorescence recovery after photobleaching, fluorescence correlation spectroscopy, fluorescence resonance energy transfer microscopy, and fluorescence lifetime imaging microscopy. These live-cell imaging tools provide an important complement to biochemical and structural biology studies, extending the analysis of protein-protein interactions, protein conformational changes, and the behavior of signaling molecules to their natural environment within the intact cell." @default.
- W2120959357 created "2016-06-24" @default.
- W2120959357 creator A5041748417 @default.
- W2120959357 creator A5045803872 @default.
- W2120959357 date "2005-07-01" @default.
- W2120959357 modified "2023-10-09" @default.
- W2120959357 title "Imaging Molecular Interactions in Living Cells" @default.
- W2120959357 cites W1481483713 @default.
- W2120959357 cites W1487368795 @default.
- W2120959357 cites W1514274123 @default.
- W2120959357 cites W1526551292 @default.
- W2120959357 cites W1546776653 @default.
- W2120959357 cites W1559720000 @default.
- W2120959357 cites W1596797500 @default.
- W2120959357 cites W1853569464 @default.
- W2120959357 cites W1958433286 @default.
- W2120959357 cites W1963498895 @default.
- W2120959357 cites W1965797967 @default.
- W2120959357 cites W1971880438 @default.
- W2120959357 cites W1975210552 @default.
- W2120959357 cites W1978122953 @default.
- W2120959357 cites W1981184494 @default.
- W2120959357 cites W1985135700 @default.
- W2120959357 cites W1985341596 @default.
- W2120959357 cites W1985701554 @default.
- W2120959357 cites W1986752048 @default.
- W2120959357 cites W1988326309 @default.
- W2120959357 cites W1989023229 @default.
- W2120959357 cites W1991934631 @default.
- W2120959357 cites W1993687714 @default.
- W2120959357 cites W1996160970 @default.
- W2120959357 cites W1996880521 @default.
- W2120959357 cites W1998506149 @default.
- W2120959357 cites W1999056422 @default.
- W2120959357 cites W2001624434 @default.
- W2120959357 cites W2003693532 @default.
- W2120959357 cites W2005841082 @default.
- W2120959357 cites W2007893737 @default.
- W2120959357 cites W2009009400 @default.
- W2120959357 cites W2009654376 @default.
- W2120959357 cites W2010820473 @default.
- W2120959357 cites W2011054400 @default.
- W2120959357 cites W2013412105 @default.
- W2120959357 cites W2017934591 @default.
- W2120959357 cites W2019405449 @default.
- W2120959357 cites W2021027041 @default.
- W2120959357 cites W2025367366 @default.
- W2120959357 cites W2027315090 @default.
- W2120959357 cites W2030347528 @default.
- W2120959357 cites W2035726898 @default.
- W2120959357 cites W2037228253 @default.
- W2120959357 cites W2038208040 @default.
- W2120959357 cites W2039576757 @default.
- W2120959357 cites W2050106427 @default.
- W2120959357 cites W2055864348 @default.
- W2120959357 cites W2056768791 @default.
- W2120959357 cites W2064613822 @default.
- W2120959357 cites W2068924803 @default.
- W2120959357 cites W2069496952 @default.
- W2120959357 cites W2071901592 @default.
- W2120959357 cites W2074204566 @default.
- W2120959357 cites W2079968973 @default.
- W2120959357 cites W2080174206 @default.
- W2120959357 cites W2080250442 @default.
- W2120959357 cites W2082205377 @default.
- W2120959357 cites W2083087411 @default.
- W2120959357 cites W2084424064 @default.
- W2120959357 cites W2092324010 @default.
- W2120959357 cites W2093287522 @default.
- W2120959357 cites W2093487214 @default.
- W2120959357 cites W2096594220 @default.
- W2120959357 cites W2097371203 @default.
- W2120959357 cites W2099546976 @default.
- W2120959357 cites W2101785963 @default.
- W2120959357 cites W2111455017 @default.
- W2120959357 cites W2111518741 @default.
- W2120959357 cites W2112514675 @default.
- W2120959357 cites W2115734610 @default.
- W2120959357 cites W2116435209 @default.
- W2120959357 cites W2119096223 @default.
- W2120959357 cites W2120622970 @default.
- W2120959357 cites W2121132820 @default.
- W2120959357 cites W2126149754 @default.
- W2120959357 cites W2127810309 @default.
- W2120959357 cites W2129096696 @default.
- W2120959357 cites W2135374895 @default.
- W2120959357 cites W2142090085 @default.
- W2120959357 cites W2142519388 @default.
- W2120959357 cites W2144153233 @default.
- W2120959357 cites W2145961463 @default.
- W2120959357 cites W2146470265 @default.
- W2120959357 cites W2147658507 @default.
- W2120959357 cites W2148493274 @default.
- W2120959357 cites W2149372476 @default.
- W2120959357 cites W2149644756 @default.
- W2120959357 cites W2153335351 @default.
- W2120959357 cites W2154298653 @default.
- W2120959357 cites W2156038004 @default.