FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2120971737> ?p ?o ?g. }

• W2120971737 endingPage "744" @default.
• W2120971737 startingPage "741" @default.
• W2120971737 abstract "Abstract Immunoprotection of pancreatic islets for successful allo‐ or xenotransplantation without chronic immunosuppression is an attractive, but still elusive, approach for curing type 1 diabetes. It was recently shown that, even in the absence of fibrotic overgrowth, other factors, mainly insufficient nutrition to the core of the islets, represent a major barrier for long‐term survival of intraperitoneal microencapsulated islet grafts. The use of dispersed cells might contribute to solve this problem due to the conceivably easier nutritional support to the cells. In the present study, purified bovine islets, prepared by collagenase digestion and density gradient purification, and dispersed bovine islet cells, obtained by trypsin and DNAsi (viability > 90%), were entrapped into either 2% (w/v) sodium alginate (commonly used for encapsulation purposes) or (dispersed islet cells only) macroporous gelatin microcarriers (CulthiSpher‐S, commonly used for the production of biologicals by animal cells). Insulin release studies in response to glucose were performed within 1 week and after 1 month from preparation of the varying systems and showed no capability of dispersed bovine islet cells within sodium alginate microcapsules to sense glucose concentration changes. On the contrary, bovine islet cells entrapped in CulthiSpher‐S microcarriers showed maintained capacity of increasing insulin secretion upon enhanced glucose concentration challenge. In this case, insulin release was approximately 60% of that from intact bovine islets within sodium alginate microcapsules. MTT and hematoxylineosin staining of islet cell‐containing microcarriers showed the presence of viable and metabolically active cells throughout the study period. This encouraging functional data prompted us to test whether the microcarriers could be immunoisolated for potential use in transplantation. The microcarriers were embedded within 3% sodium alginate, which was then covered with a poly‐L‐lysine layer and a final outer alginate layer. Maintained insulin secretion function of this system was observed, which raises the possibility of using microencapsulated CulthiSpher‐S microcarriers, containing dispersed pancreatic islet cells, in experimental transplantation studies. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 75: 741–744, 2001." @default.
• W2120971737 created "2016-06-24" @default.
• W2120971737 creator A5000550995 @default.
• W2120971737 creator A5035034573 @default.
• W2120971737 creator A5048301417 @default.
• W2120971737 creator A5050905814 @default.
• W2120971737 creator A5054574491 @default.
• W2120971737 creator A5075183342 @default.
• W2120971737 creator A5080129058 @default.
• W2120971737 creator A5086505230 @default.
• W2120971737 creator A5090610183 @default.
• W2120971737 date "2001-11-08" @default.
• W2120971737 modified "2023-10-18" @default.
• W2120971737 title "Entrapment of dispersed pancreatic islet cells in CultiSpher-S macroporous gelatin microcarriers: Preparation, in vitro characterization, and microencapsulation" @default.
• W2120971737 cites W1459437705 @default.
• W2120971737 cites W1578136772 @default.
• W2120971737 cites W1986169709 @default.
• W2120971737 cites W2019283908 @default.
• W2120971737 cites W2076804771 @default.
• W2120971737 cites W2080023216 @default.
• W2120971737 cites W2092803859 @default.
• W2120971737 cites W2098899709 @default.
• W2120971737 cites W2116708491 @default.
• W2120971737 cites W2128762381 @default.
• W2120971737 cites W2158554737 @default.
• W2120971737 cites W4210962069 @default.
• W2120971737 doi "https://doi.org/10.1002/bit.10053" @default.
• W2120971737 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11745153" @default.
• W2120971737 hasPublicationYear "2001" @default.
• W2120971737 type Work @default.
• W2120971737 sameAs 2120971737 @default.
• W2120971737 citedByCount "51" @default.
• W2120971737 countsByYear W21209717372012 @default.
• W2120971737 countsByYear W21209717372013 @default.
• W2120971737 countsByYear W21209717372014 @default.
• W2120971737 countsByYear W21209717372015 @default.
• W2120971737 countsByYear W21209717372017 @default.
• W2120971737 countsByYear W21209717372018 @default.
• W2120971737 countsByYear W21209717372019 @default.
• W2120971737 countsByYear W21209717372020 @default.
• W2120971737 countsByYear W21209717372021 @default.
• W2120971737 countsByYear W21209717372022 @default.
• W2120971737 crossrefType "journal-article" @default.
• W2120971737 hasAuthorship W2120971737A5000550995 @default.
• W2120971737 hasAuthorship W2120971737A5035034573 @default.
• W2120971737 hasAuthorship W2120971737A5048301417 @default.
• W2120971737 hasAuthorship W2120971737A5050905814 @default.
• W2120971737 hasAuthorship W2120971737A5054574491 @default.
• W2120971737 hasAuthorship W2120971737A5075183342 @default.
• W2120971737 hasAuthorship W2120971737A5080129058 @default.
• W2120971737 hasAuthorship W2120971737A5086505230 @default.
• W2120971737 hasAuthorship W2120971737A5090610183 @default.
• W2120971737 hasConcept C126322002 @default.
• W2120971737 hasConcept C134018914 @default.
• W2120971737 hasConcept C1491633281 @default.
• W2120971737 hasConcept C165220095 @default.
• W2120971737 hasConcept C176999471 @default.
• W2120971737 hasConcept C181199279 @default.
• W2120971737 hasConcept C185592680 @default.
• W2120971737 hasConcept C202751555 @default.
• W2120971737 hasConcept C2777339698 @default.
• W2120971737 hasConcept C2778394429 @default.
• W2120971737 hasConcept C2778442404 @default.
• W2120971737 hasConcept C2778823387 @default.
• W2120971737 hasConcept C2779306644 @default.
• W2120971737 hasConcept C2779855799 @default.
• W2120971737 hasConcept C2780340462 @default.
• W2120971737 hasConcept C2911091166 @default.
• W2120971737 hasConcept C38240268 @default.
• W2120971737 hasConcept C55493867 @default.
• W2120971737 hasConcept C71924100 @default.
• W2120971737 hasConcept C86803240 @default.
• W2120971737 hasConceptScore W2120971737C126322002 @default.
• W2120971737 hasConceptScore W2120971737C134018914 @default.
• W2120971737 hasConceptScore W2120971737C1491633281 @default.
• W2120971737 hasConceptScore W2120971737C165220095 @default.
• W2120971737 hasConceptScore W2120971737C176999471 @default.
• W2120971737 hasConceptScore W2120971737C181199279 @default.
• W2120971737 hasConceptScore W2120971737C185592680 @default.
• W2120971737 hasConceptScore W2120971737C202751555 @default.
• W2120971737 hasConceptScore W2120971737C2777339698 @default.
• W2120971737 hasConceptScore W2120971737C2778394429 @default.
• W2120971737 hasConceptScore W2120971737C2778442404 @default.
• W2120971737 hasConceptScore W2120971737C2778823387 @default.
• W2120971737 hasConceptScore W2120971737C2779306644 @default.
• W2120971737 hasConceptScore W2120971737C2779855799 @default.
• W2120971737 hasConceptScore W2120971737C2780340462 @default.
• W2120971737 hasConceptScore W2120971737C2911091166 @default.
• W2120971737 hasConceptScore W2120971737C38240268 @default.
• W2120971737 hasConceptScore W2120971737C55493867 @default.
• W2120971737 hasConceptScore W2120971737C71924100 @default.
• W2120971737 hasConceptScore W2120971737C86803240 @default.
• W2120971737 hasIssue "6" @default.
• W2120971737 hasLocation W21209717371 @default.
• W2120971737 hasLocation W21209717372 @default.
• W2120971737 hasOpenAccess W2120971737 @default.
• W2120971737 hasPrimaryLocation W21209717371 @default.
• W2120971737 hasRelatedWork W135003083 @default.

• next