FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2121026113> ?p ?o ?g. }

• W2121026113 endingPage "708" @default.
• W2121026113 startingPage "700" @default.
• W2121026113 abstract "Objective: This study investigated whether endocardial endothelial cells contribute to intracellular pH (pHi) regulation of subjacent cardiomyocytes. Methods: Right ventricular rabbit papillary muscles were loaded with the pH-sensitive dye 2′,7′-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethyl ester (BCECF-AM) to measure pHi and HCO3− equivalent influx or efflux in muscles with intact endocardial endothelium (+EE) and in muscles with the endocardial endothelium removed (−EE). Results: In steady-state conditions, pHi was consistently higher in +EE than in −EE muscles (7.38±0.03, n=39 vs. 7.27±0.04, n=20, p<0.05). In +EE muscles, removal of HCO3− from the buffer solution or adding 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid (DIDS), an inhibitor of HCO3− transport, reduced pHi from 7.38 to 7.24±0.06 (n=14) and to 7.16±0.10 (n=10), respectively, whereas in −EE muscles pHi decreased slightly from 7.27 to 7.15±0.05 (n=14) and to 7.13±0.04 (n=7). In addition, HCO3− equivalent efflux during alkali loads by NH4Cl pulses was smaller in +EE muscles than in −EE muscles (0.89±0.50 vs. 1.99±0.12 mmol/l/min, n=5, p<0.05) and was inhibited by DIDS. HCO3− equivalent influx during recovery from acid load imposed upon wash out of NH4Cl, was larger in +EE muscles than in −EE muscles (2.15±0.54 mmol/l/min, n=6, vs. 1.06±0.20 mmol/l/min, n=5). 5-(N-Ethyl-N-isopropyl)amiloride (EIPA), an inhibitor of Na+/H+ exchange, decreased HCO3− equivalent influx by 50% in both muscle groups but influx was still significantly higher in +EE than in −EE muscles (1.18±0.23 vs. 0.57±0.07 mmol/l/min, p<0.05). Finally, endocardial endothelial cells cultured on collagen-coated inserts established a DIDS-sensitive transendothelial HCO3−gradient. Conclusion: These data suggest that the endocardial endothelium maintains transendothelial fluxes of HCO3− from luminal (blood) to basal (muscle) side of the cells, which modulate pHi regulation in subjacent cardiomyocytes." @default.
• W2121026113 created "2016-06-24" @default.
• W2121026113 creator A5022465050 @default.
• W2121026113 creator A5054708047 @default.
• W2121026113 creator A5078498544 @default.
• W2121026113 creator A5089508602 @default.
• W2121026113 date "2004-09-01" @default.
• W2121026113 modified "2023-10-17" @default.
• W2121026113 title "Endocardial endothelium modulates subendocardial pHi of rabbit papillary muscles: role of transendothelial HCO3? transport" @default.
• W2121026113 cites W127657894 @default.
• W2121026113 cites W1966647373 @default.
• W2121026113 cites W1974514289 @default.
• W2121026113 cites W1985277152 @default.
• W2121026113 cites W1987812611 @default.
• W2121026113 cites W2028847905 @default.
• W2121026113 cites W2033590064 @default.
• W2121026113 cites W2033936610 @default.
• W2121026113 cites W2040111193 @default.
• W2121026113 cites W2049271726 @default.
• W2121026113 cites W2053989268 @default.
• W2121026113 cites W2060510699 @default.
• W2121026113 cites W2067977003 @default.
• W2121026113 cites W2073440548 @default.
• W2121026113 cites W2076244849 @default.
• W2121026113 cites W2078193143 @default.
• W2121026113 cites W2099488952 @default.
• W2121026113 cites W2102024047 @default.
• W2121026113 cites W2105166440 @default.
• W2121026113 cites W2115471525 @default.
• W2121026113 cites W2125828952 @default.
• W2121026113 cites W2139198003 @default.
• W2121026113 cites W2146041773 @default.
• W2121026113 cites W2148945565 @default.
• W2121026113 cites W2155439693 @default.
• W2121026113 cites W2160542728 @default.
• W2121026113 cites W2169261656 @default.
• W2121026113 cites W2171952998 @default.
• W2121026113 cites W2184179008 @default.
• W2121026113 cites W2188576260 @default.
• W2121026113 cites W2247294101 @default.
• W2121026113 cites W2286152392 @default.
• W2121026113 cites W2313775743 @default.
• W2121026113 cites W2338991699 @default.
• W2121026113 cites W2394829738 @default.
• W2121026113 cites W2395080552 @default.
• W2121026113 doi "https://doi.org/10.1016/j.cardiores.2004.05.017" @default.
• W2121026113 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15306226" @default.
• W2121026113 hasPublicationYear "2004" @default.
• W2121026113 type Work @default.
• W2121026113 sameAs 2121026113 @default.
• W2121026113 citedByCount "5" @default.
• W2121026113 crossrefType "journal-article" @default.
• W2121026113 hasAuthorship W2121026113A5022465050 @default.
• W2121026113 hasAuthorship W2121026113A5054708047 @default.
• W2121026113 hasAuthorship W2121026113A5078498544 @default.
• W2121026113 hasAuthorship W2121026113A5089508602 @default.
• W2121026113 hasBestOaLocation W21210261131 @default.
• W2121026113 hasConcept C105702510 @default.
• W2121026113 hasConcept C12554922 @default.
• W2121026113 hasConcept C126322002 @default.
• W2121026113 hasConcept C134018914 @default.
• W2121026113 hasConcept C185592680 @default.
• W2121026113 hasConcept C2776992346 @default.
• W2121026113 hasConcept C2779533339 @default.
• W2121026113 hasConcept C28406088 @default.
• W2121026113 hasConcept C41625074 @default.
• W2121026113 hasConcept C55493867 @default.
• W2121026113 hasConcept C71924100 @default.
• W2121026113 hasConcept C86803240 @default.
• W2121026113 hasConceptScore W2121026113C105702510 @default.
• W2121026113 hasConceptScore W2121026113C12554922 @default.
• W2121026113 hasConceptScore W2121026113C126322002 @default.
• W2121026113 hasConceptScore W2121026113C134018914 @default.
• W2121026113 hasConceptScore W2121026113C185592680 @default.
• W2121026113 hasConceptScore W2121026113C2776992346 @default.
• W2121026113 hasConceptScore W2121026113C2779533339 @default.
• W2121026113 hasConceptScore W2121026113C28406088 @default.
• W2121026113 hasConceptScore W2121026113C41625074 @default.
• W2121026113 hasConceptScore W2121026113C55493867 @default.
• W2121026113 hasConceptScore W2121026113C71924100 @default.
• W2121026113 hasConceptScore W2121026113C86803240 @default.
• W2121026113 hasIssue "4" @default.
• W2121026113 hasLocation W21210261131 @default.
• W2121026113 hasLocation W21210261132 @default.
• W2121026113 hasOpenAccess W2121026113 @default.
• W2121026113 hasPrimaryLocation W21210261131 @default.
• W2121026113 hasRelatedWork W1520627554 @default.
• W2121026113 hasRelatedWork W2022993842 @default.
• W2121026113 hasRelatedWork W2028245414 @default.
• W2121026113 hasRelatedWork W2048633187 @default.
• W2121026113 hasRelatedWork W2063342945 @default.
• W2121026113 hasRelatedWork W2068485613 @default.
• W2121026113 hasRelatedWork W2078538856 @default.
• W2121026113 hasRelatedWork W2081434191 @default.
• W2121026113 hasRelatedWork W2972580600 @default.
• W2121026113 hasRelatedWork W4234616360 @default.
• W2121026113 hasVolume "63" @default.
• W2121026113 isParatext "false" @default.

• next