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• W2121149235 startingPage "152" @default.
• W2121149235 abstract "We have reviewed the literature to identify and characterize reports of warm-antibody type, autoimmune hemolytic anemia in which the standard direct antiglobulin reaction was negative but a confirmatory test indicated that the red cells were opsonized with antibody. Three principal reasons account for the absence of a positive direct antiglobulin test in these cases: a) IgG sensitization below the threshold of detection by the commercial antiglobulin reagent, b) low affinity IgG, removed by preparatory washes not conducted at 4°C or at low ionic strength, and c) red cell sensitization by IgA alone, or rarely (monomeric) IgM alone, but not accompanied by complement fixation, and thus not detectable by a commercial antiglobulin reagent that contains anti-IgG and anti-C3. In cases in which the phenotype is compatible with warm-antibody type, autoimmune hemolytic anemia and the direct antiglobulin test is negative, an alternative method to detect low levels of IgG sensitization, use of 4°C, low ionic strength washes to prepare the cells for the direct antiglobulin test reaction to permit retention and identification of low affinity IgG antibodies, and, if the latter are uninformative, testing for sensitization with an anti-IgA, and, if necessary, an anti-IgM reagent identifies cases of warm-antibody type, immune hemolysis not verified by a commercial reagent." @default.
• W2121149235 created "2016-06-24" @default.
• W2121149235 creator A5022401670 @default.
• W2121149235 creator A5082653656 @default.
• W2121149235 date "2014-04-01" @default.
• W2121149235 modified "2023-10-17" @default.
• W2121149235 title "Direct antiglobulin (“Coombs”) test-negative autoimmune hemolytic anemia: A review" @default.
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• W2121149235 doi "https://doi.org/10.1016/j.bcmd.2013.12.003" @default.
• W2121149235 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24411920" @default.
• W2121149235 hasPublicationYear "2014" @default.
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