FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2121250147> ?p ?o ?g. }

• W2121250147 endingPage "294" @default.
• W2121250147 startingPage "281" @default.
• W2121250147 abstract "It is of great interest for gene therapy to develop vectors that drive the insertion of a therapeutic gene into a chosen specific site on the cellular genome. Adeno-associated virus (AAV) is unique among mammalian viruses in that it integrates into a distinct region of human chromosome 19 (integration site AAVS1). The inverted terminal repeats (ITRs) flanking the AAV genome and the AAV-encoded nonstructural proteins Rep78 and/or Rep68 are the only viral elements necessary and sufficient for site-specific integration. However, it is also known that unrestrained Rep activity may cause nonspecific genomic rearrangements at AAVS1 and/or have detrimental effects on cell physiology. In this paper we describe the generation of a ligand-dependent form of Rep, obtained by fusing a C-terminally deleted Rep68 with a truncated form of the hormone binding domain of the human progesterone receptor, which does not bind progesterone but binds only its synthetic antagonist RU486. The activity of this chimeric protein, named Rep1-491/P, is highly dependent on RU486 in various assays: in particular, it triggers site-specific integration at AAVS1 of an ITR-flanked cassette in a ligand-dependent manner, as efficiently as wild-type Rep68 but without generating unwanted genomic rearrangement at AAVS1." @default.
• W2121250147 created "2016-06-24" @default.
• W2121250147 creator A5015058883 @default.
• W2121250147 creator A5015441971 @default.
• W2121250147 creator A5052593572 @default.
• W2121250147 creator A5073667774 @default.
• W2121250147 creator A5075646409 @default.
• W2121250147 date "2000-01-01" @default.
• W2121250147 modified "2023-10-10" @default.
• W2121250147 title "Conditional Site-Specific Integration into Human Chromosome 19 by Using a Ligand-Dependent Chimeric Adeno-Associated Virus/Rep Protein" @default.
• W2121250147 cites W106506922 @default.
• W2121250147 cites W1505360662 @default.
• W2121250147 cites W1522993284 @default.
• W2121250147 cites W1539443568 @default.
• W2121250147 cites W1544405851 @default.
• W2121250147 cites W1554330891 @default.
• W2121250147 cites W1572423013 @default.
• W2121250147 cites W1604836384 @default.
• W2121250147 cites W1645009826 @default.
• W2121250147 cites W1648736195 @default.
• W2121250147 cites W1670213317 @default.
• W2121250147 cites W1679811511 @default.
• W2121250147 cites W170859630 @default.
• W2121250147 cites W1718708463 @default.
• W2121250147 cites W174638640 @default.
• W2121250147 cites W1755517856 @default.
• W2121250147 cites W1797988358 @default.
• W2121250147 cites W1807225431 @default.
• W2121250147 cites W1826524642 @default.
• W2121250147 cites W1827934451 @default.
• W2121250147 cites W1906949314 @default.
• W2121250147 cites W1912592927 @default.
• W2121250147 cites W1950706016 @default.
• W2121250147 cites W1967179182 @default.
• W2121250147 cites W1967446284 @default.
• W2121250147 cites W1977113550 @default.
• W2121250147 cites W1994595940 @default.
• W2121250147 cites W2004428104 @default.
• W2121250147 cites W2007048730 @default.
• W2121250147 cites W2007095985 @default.
• W2121250147 cites W2010126361 @default.
• W2121250147 cites W2012812971 @default.
• W2121250147 cites W2016782363 @default.
• W2121250147 cites W2023765807 @default.
• W2121250147 cites W2024163833 @default.
• W2121250147 cites W2026237709 @default.
• W2121250147 cites W2028043537 @default.
• W2121250147 cites W2037404178 @default.
• W2121250147 cites W2044005085 @default.
• W2121250147 cites W2046262447 @default.
• W2121250147 cites W2058855160 @default.
• W2121250147 cites W2068113812 @default.
• W2121250147 cites W2070535450 @default.
• W2121250147 cites W2090781939 @default.
• W2121250147 cites W2096466927 @default.
• W2121250147 cites W2101518847 @default.
• W2121250147 cites W2103928212 @default.
• W2121250147 cites W2105014300 @default.
• W2121250147 cites W2111124876 @default.
• W2121250147 cites W2121034113 @default.
• W2121250147 cites W2121863846 @default.
• W2121250147 cites W2125422350 @default.
• W2121250147 cites W2128595345 @default.
• W2121250147 cites W2128840922 @default.
• W2121250147 cites W2130506552 @default.
• W2121250147 cites W2135890914 @default.
• W2121250147 cites W2140523898 @default.
• W2121250147 cites W2140963402 @default.
• W2121250147 cites W2143320553 @default.
• W2121250147 cites W2151536765 @default.
• W2121250147 cites W2159648706 @default.
• W2121250147 cites W2159720414 @default.
• W2121250147 cites W2167223040 @default.
• W2121250147 cites W2412987104 @default.
• W2121250147 cites W4252035435 @default.
• W2121250147 cites W4256306656 @default.
• W2121250147 cites W4323238558 @default.
• W2121250147 cites W69516432 @default.
• W2121250147 cites W856065083 @default.
• W2121250147 doi "https://doi.org/10.1128/jvi.74.1.281-294.2000" @default.
• W2121250147 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/111538" @default.
• W2121250147 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10590116" @default.
• W2121250147 hasPublicationYear "2000" @default.
• W2121250147 type Work @default.
• W2121250147 sameAs 2121250147 @default.
• W2121250147 citedByCount "33" @default.
• W2121250147 countsByYear W21212501472012 @default.
• W2121250147 countsByYear W21212501472014 @default.
• W2121250147 countsByYear W21212501472016 @default.
• W2121250147 crossrefType "journal-article" @default.
• W2121250147 hasAuthorship W2121250147A5015058883 @default.
• W2121250147 hasAuthorship W2121250147A5015441971 @default.
• W2121250147 hasAuthorship W2121250147A5052593572 @default.
• W2121250147 hasAuthorship W2121250147A5073667774 @default.
• W2121250147 hasAuthorship W2121250147A5075646409 @default.
• W2121250147 hasBestOaLocation W21212501471 @default.
• W2121250147 hasConcept C104317684 @default.
• W2121250147 hasConcept C123894998 @default.

• next