Matches in SemOpenAlex for { <https://semopenalex.org/work/W2121454258> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2121454258 endingPage "48" @default.
- W2121454258 startingPage "39" @default.
- W2121454258 abstract "The hydroxysteroid dehydrogenases: β-HSDH, 20β-HSDH, and 3α-HSDH, were immobilized on CNBr-activated Sepharose. The effect of various immobilization conditions on the activity recovery and stability were examined. The presence of cofactor during the immobilization reaction increased the activity recovery (40–60% of the total) and also led to materials highly stable in the presence of organic solvents. For example, β-HSDH maintained 60% of its original activity two months after continuous use in the water–ethyl-acetate system. Kinetic experiments showed that the increase of the apparent Km values is poor and demonstrated that the organic solvent behaves as a weak inhibitor (ki > 0.2M) for the substrate. The immobilized enzymes lyophilized in the presence of sucrose had full activity restored even after several months storage at room temperature. Immobilized hydroxysteroid dehydrogenases were shown to be suitable for preparative transformation of steroids in water–organic solvent systems." @default.
- W2121454258 created "2016-06-24" @default.
- W2121454258 creator A5046926553 @default.
- W2121454258 creator A5052988170 @default.
- W2121454258 creator A5073641797 @default.
- W2121454258 creator A5078893957 @default.
- W2121454258 creator A5087021373 @default.
- W2121454258 date "1979-01-01" @default.
- W2121454258 modified "2023-09-23" @default.
- W2121454258 title "Immobilized hydroxysteroid dehydrogenases for the transformation of steroids in water-organic solvent systems" @default.
- W2121454258 cites W1982075420 @default.
- W2121454258 cites W2026602711 @default.
- W2121454258 cites W2033678773 @default.
- W2121454258 cites W2064985385 @default.
- W2121454258 cites W2080779467 @default.
- W2121454258 cites W2099216877 @default.
- W2121454258 cites W2152593177 @default.
- W2121454258 cites W4236047241 @default.
- W2121454258 doi "https://doi.org/10.1002/bit.260210104" @default.
- W2121454258 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/427261" @default.
- W2121454258 hasPublicationYear "1979" @default.
- W2121454258 type Work @default.
- W2121454258 sameAs 2121454258 @default.
- W2121454258 citedByCount "48" @default.
- W2121454258 countsByYear W21214542582015 @default.
- W2121454258 countsByYear W21214542582017 @default.
- W2121454258 countsByYear W21214542582019 @default.
- W2121454258 countsByYear W21214542582022 @default.
- W2121454258 crossrefType "journal-article" @default.
- W2121454258 hasAuthorship W2121454258A5046926553 @default.
- W2121454258 hasAuthorship W2121454258A5052988170 @default.
- W2121454258 hasAuthorship W2121454258A5073641797 @default.
- W2121454258 hasAuthorship W2121454258A5078893957 @default.
- W2121454258 hasAuthorship W2121454258A5087021373 @default.
- W2121454258 hasConcept C111368507 @default.
- W2121454258 hasConcept C127313418 @default.
- W2121454258 hasConcept C127413603 @default.
- W2121454258 hasConcept C181199279 @default.
- W2121454258 hasConcept C185592680 @default.
- W2121454258 hasConcept C197957613 @default.
- W2121454258 hasConcept C2776317432 @default.
- W2121454258 hasConcept C2777289219 @default.
- W2121454258 hasConcept C2779858419 @default.
- W2121454258 hasConcept C2779881004 @default.
- W2121454258 hasConcept C2780471494 @default.
- W2121454258 hasConcept C2908857472 @default.
- W2121454258 hasConcept C3020166856 @default.
- W2121454258 hasConcept C33633552 @default.
- W2121454258 hasConcept C42360764 @default.
- W2121454258 hasConcept C43617362 @default.
- W2121454258 hasConcept C55493867 @default.
- W2121454258 hasConceptScore W2121454258C111368507 @default.
- W2121454258 hasConceptScore W2121454258C127313418 @default.
- W2121454258 hasConceptScore W2121454258C127413603 @default.
- W2121454258 hasConceptScore W2121454258C181199279 @default.
- W2121454258 hasConceptScore W2121454258C185592680 @default.
- W2121454258 hasConceptScore W2121454258C197957613 @default.
- W2121454258 hasConceptScore W2121454258C2776317432 @default.
- W2121454258 hasConceptScore W2121454258C2777289219 @default.
- W2121454258 hasConceptScore W2121454258C2779858419 @default.
- W2121454258 hasConceptScore W2121454258C2779881004 @default.
- W2121454258 hasConceptScore W2121454258C2780471494 @default.
- W2121454258 hasConceptScore W2121454258C2908857472 @default.
- W2121454258 hasConceptScore W2121454258C3020166856 @default.
- W2121454258 hasConceptScore W2121454258C33633552 @default.
- W2121454258 hasConceptScore W2121454258C42360764 @default.
- W2121454258 hasConceptScore W2121454258C43617362 @default.
- W2121454258 hasConceptScore W2121454258C55493867 @default.
- W2121454258 hasIssue "1" @default.
- W2121454258 hasLocation W21214542581 @default.
- W2121454258 hasLocation W21214542582 @default.
- W2121454258 hasOpenAccess W2121454258 @default.
- W2121454258 hasPrimaryLocation W21214542581 @default.
- W2121454258 hasRelatedWork W1164537646 @default.
- W2121454258 hasRelatedWork W169735115 @default.
- W2121454258 hasRelatedWork W1995541999 @default.
- W2121454258 hasRelatedWork W2000655475 @default.
- W2121454258 hasRelatedWork W2039430749 @default.
- W2121454258 hasRelatedWork W2080203695 @default.
- W2121454258 hasRelatedWork W2121454258 @default.
- W2121454258 hasRelatedWork W2443727728 @default.
- W2121454258 hasRelatedWork W3022531063 @default.
- W2121454258 hasRelatedWork W3087653025 @default.
- W2121454258 hasVolume "21" @default.
- W2121454258 isParatext "false" @default.
- W2121454258 isRetracted "false" @default.
- W2121454258 magId "2121454258" @default.
- W2121454258 workType "article" @default.