FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2121460090> ?p ?o ?g. }

• W2121460090 endingPage "L1069" @default.
• W2121460090 startingPage "L1059" @default.
• W2121460090 abstract "Hypoxic pulmonary vasoconstriction (HPV) requires influx of extracellular Ca 2+ in pulmonary arterial smooth muscle cells (PASMCs). To determine whether capacitative Ca 2+ entry (CCE) through store-operated Ca 2+ channels (SOCCs) contributes to this influx, we used fluorescent microscopy and the Ca 2+ -sensitive dye fura-2 to measure effects of 4% O 2 on intracellular [Ca 2+ ] ([Ca 2+ ] i ) and CCE in primary cultures of PASMCs from rat distal pulmonary arteries. In PASMCs perfused with Ca 2+ -free Krebs Ringer bicarbonate solution (KRBS) containing cyclopiazonic acid to deplete Ca 2+ stores in sarcoplasmic reticulum and nifedipine to prevent Ca 2+ entry through L-type voltage-operated Ca 2+ channels (VOCCs), hypoxia markedly enhanced both the increase in [Ca 2+ ] i caused by restoration of extracellular [Ca 2+ ] and the rate at which extracellular Mn 2+ quenched fura-2 fluorescence. These effects, as well as the increased [Ca 2+ ] i caused by hypoxia in PASMCs perfused with normal salt solutions, were blocked by the SOCC antagonists SKF-96365, NiCl 2 , and LaCl 3 at concentrations that inhibited CCE >80% but did not alter [Ca 2+ ] i responses to 60 mM KCl. In contrast, the VOCC antagonist nifedipine inhibited [Ca 2+ ] i responses to hypoxia by only 50% at concentrations that completely blocked responses to KCl. The increased [Ca 2+ ] i caused by hypoxia was completely reversed by perfusion with Ca 2+ -free KRBS. LaCl 3 increased basal [Ca 2+ ] i during normoxia, indicating effects other than inhibition of SOCCs. Our results suggest that acute hypoxia enhances CCE through SOCCs in distal PASMCs, leading to depolarization, secondary activation of VOCCs, and increased [Ca 2+ ] i . SOCCs and CCE may play important roles in HPV." @default.
• W2121460090 created "2016-06-24" @default.
• W2121460090 creator A5004851279 @default.
• W2121460090 creator A5015208632 @default.
• W2121460090 creator A5016256162 @default.
• W2121460090 creator A5028237874 @default.
• W2121460090 creator A5032764121 @default.
• W2121460090 creator A5044245877 @default.
• W2121460090 date "2005-06-01" @default.
• W2121460090 modified "2023-10-17" @default.
• W2121460090 title "Acute hypoxia increases intracellular [Ca²⁺] in pulmonary arterial smooth muscle by enhancing capacitative Ca²⁺ entry" @default.
• W2121460090 cites W145922606 @default.
• W2121460090 cites W1526317775 @default.
• W2121460090 cites W1880961408 @default.
• W2121460090 cites W1889047426 @default.
• W2121460090 cites W1926185647 @default.
• W2121460090 cites W195031074 @default.
• W2121460090 cites W1965345723 @default.
• W2121460090 cites W1969619908 @default.
• W2121460090 cites W1980829733 @default.
• W2121460090 cites W1986117743 @default.
• W2121460090 cites W1992534903 @default.
• W2121460090 cites W1994903699 @default.
• W2121460090 cites W2016010885 @default.
• W2121460090 cites W2016133715 @default.
• W2121460090 cites W2019345683 @default.
• W2121460090 cites W2020402496 @default.
• W2121460090 cites W2021340897 @default.
• W2121460090 cites W2022787032 @default.
• W2121460090 cites W2023351764 @default.
• W2121460090 cites W2023455773 @default.
• W2121460090 cites W2030966306 @default.
• W2121460090 cites W2036137960 @default.
• W2121460090 cites W2040879251 @default.
• W2121460090 cites W2041690080 @default.
• W2121460090 cites W2050099786 @default.
• W2121460090 cites W2054317829 @default.
• W2121460090 cites W2061098585 @default.
• W2121460090 cites W2064241631 @default.
• W2121460090 cites W2064686740 @default.
• W2121460090 cites W2065334521 @default.
• W2121460090 cites W2071340957 @default.
• W2121460090 cites W2085124926 @default.
• W2121460090 cites W2089440992 @default.
• W2121460090 cites W2089673017 @default.
• W2121460090 cites W2095317716 @default.
• W2121460090 cites W2098004670 @default.
• W2121460090 cites W2100501371 @default.
• W2121460090 cites W2113158482 @default.
• W2121460090 cites W2117088919 @default.
• W2121460090 cites W2117796487 @default.
• W2121460090 cites W2123823383 @default.
• W2121460090 cites W2124658641 @default.
• W2121460090 cites W2128450740 @default.
• W2121460090 cites W2137357027 @default.
• W2121460090 cites W2148806848 @default.
• W2121460090 cites W2158095602 @default.
• W2121460090 cites W2161440135 @default.
• W2121460090 cites W2165555244 @default.
• W2121460090 cites W2168380916 @default.
• W2121460090 cites W2181344473 @default.
• W2121460090 cites W2184358523 @default.
• W2121460090 cites W2186345045 @default.
• W2121460090 cites W2189572984 @default.
• W2121460090 cites W2215636382 @default.
• W2121460090 cites W2238916873 @default.
• W2121460090 cites W2252404386 @default.
• W2121460090 cites W2266546728 @default.
• W2121460090 cites W2311570504 @default.
• W2121460090 cites W2325318882 @default.
• W2121460090 cites W2335815721 @default.
• W2121460090 cites W2339884335 @default.
• W2121460090 cites W2398497680 @default.
• W2121460090 cites W2404088629 @default.
• W2121460090 cites W2408804735 @default.
• W2121460090 cites W2410077164 @default.
• W2121460090 cites W2415836019 @default.
• W2121460090 cites W2419968188 @default.
• W2121460090 cites W2472364609 @default.
• W2121460090 doi "https://doi.org/10.1152/ajplung.00448.2004" @default.
• W2121460090 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15665040" @default.
• W2121460090 hasPublicationYear "2005" @default.
• W2121460090 type Work @default.
• W2121460090 sameAs 2121460090 @default.
• W2121460090 citedByCount "129" @default.
• W2121460090 countsByYear W21214600902012 @default.
• W2121460090 countsByYear W21214600902013 @default.
• W2121460090 countsByYear W21214600902014 @default.
• W2121460090 countsByYear W21214600902015 @default.
• W2121460090 countsByYear W21214600902016 @default.
• W2121460090 countsByYear W21214600902017 @default.
• W2121460090 countsByYear W21214600902018 @default.
• W2121460090 countsByYear W21214600902019 @default.
• W2121460090 countsByYear W21214600902020 @default.
• W2121460090 countsByYear W21214600902021 @default.
• W2121460090 countsByYear W21214600902022 @default.
• W2121460090 countsByYear W21214600902023 @default.
• W2121460090 crossrefType "journal-article" @default.

• next