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• W2121575062 endingPage "5695" @default.
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• W2121575062 abstract "Estrogen has bone protective effects, but the exact mechanism behind these effects remains unclear. The aim of the present study was to identify the primary target cells in bone for the classical genomic effects of estrogens in vivo. For this purpose we have used reporter mice with a luciferase gene under the control of three estrogen-responsive elements (EREs), enabling detection of in vivo activation of gene transcription. Three-month-old ovariectomized mice were treated with a single dose (50 mug/kg) 17beta-estradiol (E2). Luciferase activity was analyzed in several tissues and in different bone marrow-derived lymphocyte enriched/depleted preparations using MacsMouse CD19 (for B lymphocytes) or CD90 (for T lymphocytes) MicroBeads (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). Histological characterization of cells with high luciferase content was performed using immunohistochemistry. Both cortical bone and bone marrow displayed a rapid (within 1 h) and pronounced E2-induced increase in luciferase activity. The luciferase activity in total bone marrow and in bone marrow depleted of lymphocytes was increased six to eight times more than in either B-lymphocyte or T-lymphocyte enriched cell fractions 4 h after the E2 injection, demonstrating that mature lymphocytes are not major direct targets for the genomic effect of estrogens in bone. Immunohistochemistry identified clear luciferase staining in hypertrophic growth plate chondrocytes, megakaryocytes, osteoblasts, and lining cells, whereas no staining was seen in proliferative chondrocyte. Although most of the osteocytes did not display any detectable luciferase staining, a subpopulation of osteocytes both in cortical and trabecular bone stained positive for luciferase. In conclusion, hypertrophic growth plate chondrocytes, megakaryocytes, osteoblasts, lining cells, and a subpopulation of osteocytes were identified to respond to estrogen via the classical ERE-mediated genomic pathway in bone. Furthermore, our findings indicate that possible direct estrogenic effects on the majority of osteocytes, not staining positive for luciferase, on proliferative chondrocytes and on mature lymphocytes are mediated by non-ERE actions." @default.
• W2121575062 created "2016-06-24" @default.
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• W2121575062 date "2007-12-01" @default.
• W2121575062 modified "2023-10-09" @default.
• W2121575062 title "Identification of Target Cells for the Genomic Effects of Estrogens in Bone" @default.
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• W2121575062 cites W1662539477 @default.
• W2121575062 cites W1963657210 @default.
• W2121575062 cites W1966641327 @default.
• W2121575062 cites W1968104734 @default.
• W2121575062 cites W1969027216 @default.
• W2121575062 cites W1981525203 @default.
• W2121575062 cites W1997364024 @default.
• W2121575062 cites W1999619355 @default.
• W2121575062 cites W2004818138 @default.
• W2121575062 cites W2008698339 @default.
• W2121575062 cites W2011887709 @default.
• W2121575062 cites W2019492239 @default.
• W2121575062 cites W2021427813 @default.
• W2121575062 cites W2028689516 @default.
• W2121575062 cites W2030811264 @default.
• W2121575062 cites W2031139244 @default.
• W2121575062 cites W2033757927 @default.
• W2121575062 cites W2034938926 @default.
• W2121575062 cites W2038997206 @default.
• W2121575062 cites W2041181827 @default.
• W2121575062 cites W2042095186 @default.
• W2121575062 cites W2043144329 @default.
• W2121575062 cites W2044939877 @default.
• W2121575062 cites W2045481917 @default.
• W2121575062 cites W2045590883 @default.
• W2121575062 cites W2045673939 @default.
• W2121575062 cites W2045805069 @default.
• W2121575062 cites W2046034334 @default.
• W2121575062 cites W2049716343 @default.
• W2121575062 cites W2055170266 @default.
• W2121575062 cites W2060567263 @default.
• W2121575062 cites W2061674718 @default.
• W2121575062 cites W2062937887 @default.
• W2121575062 cites W2070131786 @default.
• W2121575062 cites W2072173628 @default.
• W2121575062 cites W2074340418 @default.
• W2121575062 cites W2079239231 @default.
• W2121575062 cites W2082532013 @default.
• W2121575062 cites W2087407206 @default.
• W2121575062 cites W2093350076 @default.
• W2121575062 cites W2094600342 @default.
• W2121575062 cites W2095043666 @default.
• W2121575062 cites W2101051981 @default.
• W2121575062 cites W2102542717 @default.
• W2121575062 cites W2107925338 @default.
• W2121575062 cites W2110466129 @default.
• W2121575062 cites W2114293211 @default.
• W2121575062 cites W2120758873 @default.
• W2121575062 cites W2123343983 @default.
• W2121575062 cites W2123463315 @default.
• W2121575062 cites W2127559938 @default.
• W2121575062 cites W2133281510 @default.
• W2121575062 cites W2135299810 @default.
• W2121575062 cites W2136672074 @default.
• W2121575062 cites W2138975146 @default.
• W2121575062 cites W2144924096 @default.
• W2121575062 cites W2145894656 @default.
• W2121575062 cites W2146757249 @default.
• W2121575062 cites W2146818843 @default.
• W2121575062 cites W2146975650 @default.
• W2121575062 cites W2147687700 @default.
• W2121575062 cites W2150457017 @default.
• W2121575062 cites W2152754505 @default.
• W2121575062 cites W2156203312 @default.
• W2121575062 cites W2158856171 @default.
• W2121575062 cites W2159207368 @default.
• W2121575062 cites W2159674010 @default.
• W2121575062 cites W2160287478 @default.
• W2121575062 cites W2165202637 @default.
• W2121575062 cites W2170728568 @default.
• W2121575062 cites W2171434323 @default.
• W2121575062 cites W2321613759 @default.
• W2121575062 cites W4233274199 @default.
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• W2121575062 doi "https://doi.org/10.1210/en.2007-0508" @default.
• W2121575062 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17761761" @default.
• W2121575062 hasPublicationYear "2007" @default.
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