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- W2121791566 abstract "ABSTRACT A Pseudomonas aeruginosa clinical strain isolated from a patient hospitalized in a New Delhi, India, hospital was resistant to expanded-spectrum cephalosporins, imipenem, and aztreonam. A bla VEB-1 -like gene named bla VEB-1a , which codes for the extended-spectrum β-lactamase VEB-1a, was identified. The genetic environment of bla VEB-1a was peculiar: (i) no 5′ conserved sequence (5′-CS) region was present upstream of the β-lactamase gene, whereas bla VEB-1 -like genes are usually associated with class 1 integrons; (ii) bla VEB-1a was inserted between two truncated 3′-CS regions in a direct repeat; and (iii) four 135-bp repeated DNA sequences (repeated elements) were located on each side of the bla VEB-1a gene. Expression of the bla VEB-1a gene was driven by a strong promoter located in one of these repeated sequences. In addition, cloning of the β-lactamase content of this P. aeruginosa isolate followed by expression in Escherichia coli identified the naturally occurring AmpC β-lactamase and a gene encoding an OXA-2-like β-lactamase located in a class 1 integron, In78, in which an insertion sequence, IS pa7 , was inserted within its 5′-CS region." @default.
- W2121791566 created "2016-06-24" @default.
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- W2121791566 date "2004-09-01" @default.
- W2121791566 modified "2023-10-16" @default.
- W2121791566 title "Functional and Structural Characterization of the Genetic Environment of an Extended-Spectrum β-Lactamase <i>bla</i> <sub>VEB</sub> Gene from a <i>Pseudomonas aeruginosa</i> Isolate Obtained in India" @default.
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- W2121791566 doi "https://doi.org/10.1128/aac.48.9.3284-3290.2004" @default.
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