FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2121799466> ?p ?o ?g. }

• W2121799466 endingPage "315" @default.
• W2121799466 startingPage "301" @default.
• W2121799466 abstract "Intact nuclei derived from poorly or highly liver-metastatic murine large-cell lymphoma cell line RAW117 were digested to discrete subchromatin deoxyribonucleoprotein/ribonucleoprotein (DNP/RNP) complexes with Msp-1. The DNP/RNP complexes were composed of DNP/RNPs which were derived from the DNP/RNP complexes by incubation in the presence or absence of DNase-1 and subsequent isolation by two-dimensional [isoelectric focusing/sodium dodecylsulfate (SDS)] polyacrylamide gel electrophoresis (PAGE), electroelution from the gel, and removal of SDS. Approximately 450 DNP/RNPs in the two-dimensional gels corresponding to discrete spots or in some cases streaks were analyzed for the presence of v-abl, p53, c-neu, c-H-ras, β-casein, 18s rDNA, and μ-chain immunoglobulin genes using a hybridization technique. Ten DNP/RNP complexes contained tightly associated p53 DNA, whereas six contained c- or v-abl, four contained μ-chain gene, two contained c-H-ras, one contained dot-blot β-casein, two contained 18s rDNA, and c-neu was found in one of the DNP/RNPs. The DNP/RNPs were also analyzed for in vitro RNA polymerase and primase activities. To assess the potential transcription abilities of the isolated DNP/RNPs, individual DNP/RNPs or DNP/RNP mixtures (reconstituted after SDS-PAGE separation) were examined for RNA polymerase initiation and synthesis. When RNA products were formed, these were purified by extracellulose chromatography and used as back-hybridization probes for the genes of interest. The RNA products were also analyzed by RNA gel electrophoresis. RNA formation was inhibitable by actinomycin D, and the RNAs formed ranged in size from ∼ 80 kbp to ∼ 1 kbp. By mixing various DNP/RNP complexes together, different patterns of RNA synthesis were found. For example, one DNP/RNP of Mr ∼ 140,000, isoelectric point(pl) ∼ 5.8 synthesized a high molecular weight RNA in vitro that hybridized with β-casein cDNA, but β-casein is not expressed in RAW117 cells, suggesting that the silencing of the β-casein gene was negated by isolation of the DNP/RNP. Mixing this DNP/RNP with two other specific DNP/RNPs again inhibited the synthesis of β-casein RNA, suggesting that interactions between DNP/RNP complexes can result in differential RNA expression or regulation of RNA polymerases in vitro. © 1992 Wiley-Liss, Inc." @default.
• W2121799466 created "2016-06-24" @default.
• W2121799466 creator A5076024093 @default.
• W2121799466 creator A5088154120 @default.
• W2121799466 date "1992-11-01" @default.
• W2121799466 modified "2023-09-27" @default.
• W2121799466 title "Nucleoproteins derived from subnuclear RNA polymerase complexes of metastatic large-cell lymphoma cells possess transcription activities and regulatory properties in vitro" @default.
• W2121799466 cites W1024748644 @default.
• W2121799466 cites W1751409276 @default.
• W2121799466 cites W1834965417 @default.
• W2121799466 cites W1835690817 @default.
• W2121799466 cites W1844277683 @default.
• W2121799466 cites W1846936111 @default.
• W2121799466 cites W1901602297 @default.
• W2121799466 cites W1983969012 @default.
• W2121799466 cites W1993841394 @default.
• W2121799466 cites W1996744162 @default.
• W2121799466 cites W1999861460 @default.
• W2121799466 cites W2003863219 @default.
• W2121799466 cites W2006889190 @default.
• W2121799466 cites W2013098482 @default.
• W2121799466 cites W2018626566 @default.
• W2121799466 cites W2032984278 @default.
• W2121799466 cites W2047767262 @default.
• W2121799466 cites W2049791917 @default.
• W2121799466 cites W2059983828 @default.
• W2121799466 cites W2069252859 @default.
• W2121799466 cites W2070787783 @default.
• W2121799466 cites W2072140252 @default.
• W2121799466 cites W2085338003 @default.
• W2121799466 cites W2088336419 @default.
• W2121799466 cites W2089158354 @default.
• W2121799466 cites W2099114692 @default.
• W2121799466 cites W2099586097 @default.
• W2121799466 cites W2106426729 @default.
• W2121799466 cites W2117886328 @default.
• W2121799466 cites W2139671226 @default.
• W2121799466 cites W2140964058 @default.
• W2121799466 cites W2155210632 @default.
• W2121799466 cites W2272350926 @default.
• W2121799466 cites W4240705040 @default.
• W2121799466 doi "https://doi.org/10.1002/jcb.240500311" @default.
• W2121799466 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1469066" @default.
• W2121799466 hasPublicationYear "1992" @default.
• W2121799466 type Work @default.
• W2121799466 sameAs 2121799466 @default.
• W2121799466 citedByCount "3" @default.
• W2121799466 crossrefType "journal-article" @default.
• W2121799466 hasAuthorship W2121799466A5076024093 @default.
• W2121799466 hasAuthorship W2121799466A5088154120 @default.
• W2121799466 hasConcept C101762097 @default.
• W2121799466 hasConcept C104317684 @default.
• W2121799466 hasConcept C116084860 @default.
• W2121799466 hasConcept C138885662 @default.
• W2121799466 hasConcept C150194340 @default.
• W2121799466 hasConcept C153911025 @default.
• W2121799466 hasConcept C179926584 @default.
• W2121799466 hasConcept C185592680 @default.
• W2121799466 hasConcept C41895202 @default.
• W2121799466 hasConcept C552990157 @default.
• W2121799466 hasConcept C55493867 @default.
• W2121799466 hasConcept C60325843 @default.
• W2121799466 hasConcept C64350747 @default.
• W2121799466 hasConcept C67705224 @default.
• W2121799466 hasConcept C75934600 @default.
• W2121799466 hasConcept C82381507 @default.
• W2121799466 hasConcept C86803240 @default.
• W2121799466 hasConceptScore W2121799466C101762097 @default.
• W2121799466 hasConceptScore W2121799466C104317684 @default.
• W2121799466 hasConceptScore W2121799466C116084860 @default.
• W2121799466 hasConceptScore W2121799466C138885662 @default.
• W2121799466 hasConceptScore W2121799466C150194340 @default.
• W2121799466 hasConceptScore W2121799466C153911025 @default.
• W2121799466 hasConceptScore W2121799466C179926584 @default.
• W2121799466 hasConceptScore W2121799466C185592680 @default.
• W2121799466 hasConceptScore W2121799466C41895202 @default.
• W2121799466 hasConceptScore W2121799466C552990157 @default.
• W2121799466 hasConceptScore W2121799466C55493867 @default.
• W2121799466 hasConceptScore W2121799466C60325843 @default.
• W2121799466 hasConceptScore W2121799466C64350747 @default.
• W2121799466 hasConceptScore W2121799466C67705224 @default.
• W2121799466 hasConceptScore W2121799466C75934600 @default.
• W2121799466 hasConceptScore W2121799466C82381507 @default.
• W2121799466 hasConceptScore W2121799466C86803240 @default.
• W2121799466 hasIssue "3" @default.
• W2121799466 hasLocation W21217994661 @default.
• W2121799466 hasLocation W21217994662 @default.
• W2121799466 hasOpenAccess W2121799466 @default.
• W2121799466 hasPrimaryLocation W21217994661 @default.
• W2121799466 hasRelatedWork W1480676402 @default.
• W2121799466 hasRelatedWork W1505403902 @default.
• W2121799466 hasRelatedWork W1766852575 @default.
• W2121799466 hasRelatedWork W1771998047 @default.
• W2121799466 hasRelatedWork W1985770805 @default.
• W2121799466 hasRelatedWork W1998965262 @default.
• W2121799466 hasRelatedWork W2081903059 @default.
• W2121799466 hasRelatedWork W2085259812 @default.
• W2121799466 hasRelatedWork W2102182343 @default.

• next